Different spacer-arm attached magnetic nanoparticles for covalent immobilization of Jack bean urease

NURI GÜLESCI, (Gümüşhane Üniversitesi, Gümüşhane Meslek Yüksekokulu, Kimya ve Kimyasal İşleme Teknolojileri, Gümüşhane, Türkiye)
GÜZIDE YÜCEBILGIÇ, (Çukurova Üniversitesi, Fen-Edebiyat Fakültesi,Kimya Bölümü, Adana, Türkiye,)
Deniz Yildirim (Çukurova Üniversitesi, Mühendislik Fakültesi, Kimya Mühendisliği Bölümü, Adana, Türkiye.)
Türk Biyokimya Dergisi
7 1
Yıl: 2022 Cilt: 47 Sayı: 4 Sayfa Aralığı: 501 - 509 Metin Dili: İngilizce DOI: 10.1515/tjb-2021-0264 İndeks Tarihi: 29-05-2023

Different spacer-arm attached magnetic nanoparticles for covalent immobilization of Jack bean urease

Öz:
Objectives: This study aims the covalent immobilization of Jack bean urease on Fe3O4 magnetic nanoparticles via glutaraldehyde (urease@MNPs-Si-Glu) and epichlo- rohydrin (urease@MNPs-ECH) spacer arms. Methods: The optimum pH and temperature, thermal, storage and reuse stability of free and immobilized urease preparations were investigated. Thermodynamics cha- racterizations of free and immobilized urease preparations were also studied. Results: The free urease and both immobilized urease pre- parations showed maximal catalytic activity at pH 7.5. The free urease had a maximal catalytic activity at 50 °C, while the both immobilized urease preparations exhibited their maxi- mal catalytic activities at 70 °C. The urease@MNPs-Si-Glu and urease@MNP-ECH showed 2.7- and 1.9-fold higher thermal stability than the free urease at 60 °C, respectively. The free urease remained 30% of their initial activity at 4 °C, while urease@MNPs-Si-Glu and urease@MNPs-ECH retained 72 and 60% of their initial activities at the same conditions. The urease@MNPs-Si-Glu and urease@MNPs-ECH preserved 53 and 52% of their initial activities, respectively for urea hydrolysis after 20 reuses. Conclusions: The urease@MNPs-Si-Glu and urease@MNPs- ECH may find a potential application area in urea hydrolysis.
Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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  • The online version of this article offers supplementary material (https://doi.org/10.1515/tjb-2021-0264).
APA GÜLESCI N, YÜCEBILGIÇ G, Yildirim D (2022). Different spacer-arm attached magnetic nanoparticles for covalent immobilization of Jack bean urease. , 501 - 509. 10.1515/tjb-2021-0264
Chicago GÜLESCI NURI,YÜCEBILGIÇ GÜZIDE,Yildirim Deniz Different spacer-arm attached magnetic nanoparticles for covalent immobilization of Jack bean urease. (2022): 501 - 509. 10.1515/tjb-2021-0264
MLA GÜLESCI NURI,YÜCEBILGIÇ GÜZIDE,Yildirim Deniz Different spacer-arm attached magnetic nanoparticles for covalent immobilization of Jack bean urease. , 2022, ss.501 - 509. 10.1515/tjb-2021-0264
AMA GÜLESCI N,YÜCEBILGIÇ G,Yildirim D Different spacer-arm attached magnetic nanoparticles for covalent immobilization of Jack bean urease. . 2022; 501 - 509. 10.1515/tjb-2021-0264
Vancouver GÜLESCI N,YÜCEBILGIÇ G,Yildirim D Different spacer-arm attached magnetic nanoparticles for covalent immobilization of Jack bean urease. . 2022; 501 - 509. 10.1515/tjb-2021-0264
IEEE GÜLESCI N,YÜCEBILGIÇ G,Yildirim D "Different spacer-arm attached magnetic nanoparticles for covalent immobilization of Jack bean urease." , ss.501 - 509, 2022. 10.1515/tjb-2021-0264
ISNAD GÜLESCI, NURI vd. "Different spacer-arm attached magnetic nanoparticles for covalent immobilization of Jack bean urease". (2022), 501-509. https://doi.org/10.1515/tjb-2021-0264
APA GÜLESCI N, YÜCEBILGIÇ G, Yildirim D (2022). Different spacer-arm attached magnetic nanoparticles for covalent immobilization of Jack bean urease. Türk Biyokimya Dergisi, 47(4), 501 - 509. 10.1515/tjb-2021-0264
Chicago GÜLESCI NURI,YÜCEBILGIÇ GÜZIDE,Yildirim Deniz Different spacer-arm attached magnetic nanoparticles for covalent immobilization of Jack bean urease. Türk Biyokimya Dergisi 47, no.4 (2022): 501 - 509. 10.1515/tjb-2021-0264
MLA GÜLESCI NURI,YÜCEBILGIÇ GÜZIDE,Yildirim Deniz Different spacer-arm attached magnetic nanoparticles for covalent immobilization of Jack bean urease. Türk Biyokimya Dergisi, vol.47, no.4, 2022, ss.501 - 509. 10.1515/tjb-2021-0264
AMA GÜLESCI N,YÜCEBILGIÇ G,Yildirim D Different spacer-arm attached magnetic nanoparticles for covalent immobilization of Jack bean urease. Türk Biyokimya Dergisi. 2022; 47(4): 501 - 509. 10.1515/tjb-2021-0264
Vancouver GÜLESCI N,YÜCEBILGIÇ G,Yildirim D Different spacer-arm attached magnetic nanoparticles for covalent immobilization of Jack bean urease. Türk Biyokimya Dergisi. 2022; 47(4): 501 - 509. 10.1515/tjb-2021-0264
IEEE GÜLESCI N,YÜCEBILGIÇ G,Yildirim D "Different spacer-arm attached magnetic nanoparticles for covalent immobilization of Jack bean urease." Türk Biyokimya Dergisi, 47, ss.501 - 509, 2022. 10.1515/tjb-2021-0264
ISNAD GÜLESCI, NURI vd. "Different spacer-arm attached magnetic nanoparticles for covalent immobilization of Jack bean urease". Türk Biyokimya Dergisi 47/4 (2022), 501-509. https://doi.org/10.1515/tjb-2021-0264
Sayfa Oluşturulma Tarihi
24-04-2024 7:32

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