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Chitosan-based delivery of CRISPR-Cas9 plasmid in breast cancer stem cells

Tuba Canak Ipek, (Marmara Üniversitesi, Sağlık Bilimleri Enstitüsü, Farmasötik Biyoteknoloji Anabilim Dalı, İstanbul, Türkiye)
Meltem Avci-Adali, (University Hospital Tuebingen, Department of Thoracic and Cardiovascular Surgery, Tuebingen, Germany)
Ceyda EKENTOK ATICI, (Marmara Üniversitesi, Eczacılık Fakültesi, Farmasötik Biyoteknoloji Anabilim Dalı, İstanbul, Türkiye)
Emine Salva, (İnönü Üniversitesi, Eczacılık Fakültesi, Farmasötik Biyoteknoloji Anabilim Dalı, Malatya, Türkiye)
SUNA OZBAS TURAN (Marmara Üniversitesi, Eczacılık Fakültesi, Farmasötik Biyoteknoloji Anabilim Dalı, İstanbul, Türkiye)
Journal of research in pharmacy (online)
0 0
Yıl: 2023 Cilt: 27 Sayı: 1 Sayfa Aralığı: 86 - 96 Metin Dili: İngilizce DOI: 10.29228/jrp.292 İndeks Tarihi: 30-05-2023

Chitosan-based delivery of CRISPR-Cas9 plasmid in breast cancer stem cells

Öz:
Clustered regularly interspaced short palindromic repeat (CRISPR)-associated Cas9 nuclease system (CRISPR/Cas9) has emerged as a powerful toolbox for cancer therapy, serving as a gene fixed-point knock-out method. However, suitable gene carrier systems are urgently needed to encapsulate the CRISPR/Cas9 system and to improve the uptake into the cancer cells for anti-cancer therapy. In cancer therapy, breast cancer stem cells should be also targeted besides tumor cells. In this study, we prepared chitosan/CRISPR-Cas9/protamine nanoplexes and performed in vitro characterization. The results showed that the chitosan/protamine complex increased the zeta potential of the VEGF CRISPR/Cas9 plasmid from negative to positive. In vitro cell culture studies showed that VEGF silencing efficiency was 46.19% and 30.2% in MCF-7 and MCF-7s, respectively, after 7 days. The invasion capacity of cancer cells decreased significantly for both cell types. The results indicate that chitosan/VEGF CRISPR/Cas9 plasmid/protamine complexes can be used to reduce VEGF expression, leading to a decrease in the invasion capacity of breast cancer as well as breast cancer stem cells and providing proof of concept for more advanced studies, including in vivo studies, of this system.
Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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APA Canak Ipek T, Avci-Adali M, Ekentok C, Salva E, OZBAS TURAN S (2023). Chitosan-based delivery of CRISPR-Cas9 plasmid in breast cancer stem cells. , 86 - 96. 10.29228/jrp.292
Chicago Canak Ipek Tuba,Avci-Adali Meltem,Ekentok Ceyda,Salva Emine,OZBAS TURAN SUNA Chitosan-based delivery of CRISPR-Cas9 plasmid in breast cancer stem cells. (2023): 86 - 96. 10.29228/jrp.292
MLA Canak Ipek Tuba,Avci-Adali Meltem,Ekentok Ceyda,Salva Emine,OZBAS TURAN SUNA Chitosan-based delivery of CRISPR-Cas9 plasmid in breast cancer stem cells. , 2023, ss.86 - 96. 10.29228/jrp.292
AMA Canak Ipek T,Avci-Adali M,Ekentok C,Salva E,OZBAS TURAN S Chitosan-based delivery of CRISPR-Cas9 plasmid in breast cancer stem cells. . 2023; 86 - 96. 10.29228/jrp.292
Vancouver Canak Ipek T,Avci-Adali M,Ekentok C,Salva E,OZBAS TURAN S Chitosan-based delivery of CRISPR-Cas9 plasmid in breast cancer stem cells. . 2023; 86 - 96. 10.29228/jrp.292
IEEE Canak Ipek T,Avci-Adali M,Ekentok C,Salva E,OZBAS TURAN S "Chitosan-based delivery of CRISPR-Cas9 plasmid in breast cancer stem cells." , ss.86 - 96, 2023. 10.29228/jrp.292
ISNAD Canak Ipek, Tuba vd. "Chitosan-based delivery of CRISPR-Cas9 plasmid in breast cancer stem cells". (2023), 86-96. https://doi.org/10.29228/jrp.292
APA Canak Ipek T, Avci-Adali M, Ekentok C, Salva E, OZBAS TURAN S (2023). Chitosan-based delivery of CRISPR-Cas9 plasmid in breast cancer stem cells. Journal of research in pharmacy (online), 27(1), 86 - 96. 10.29228/jrp.292
Chicago Canak Ipek Tuba,Avci-Adali Meltem,Ekentok Ceyda,Salva Emine,OZBAS TURAN SUNA Chitosan-based delivery of CRISPR-Cas9 plasmid in breast cancer stem cells. Journal of research in pharmacy (online) 27, no.1 (2023): 86 - 96. 10.29228/jrp.292
MLA Canak Ipek Tuba,Avci-Adali Meltem,Ekentok Ceyda,Salva Emine,OZBAS TURAN SUNA Chitosan-based delivery of CRISPR-Cas9 plasmid in breast cancer stem cells. Journal of research in pharmacy (online), vol.27, no.1, 2023, ss.86 - 96. 10.29228/jrp.292
AMA Canak Ipek T,Avci-Adali M,Ekentok C,Salva E,OZBAS TURAN S Chitosan-based delivery of CRISPR-Cas9 plasmid in breast cancer stem cells. Journal of research in pharmacy (online). 2023; 27(1): 86 - 96. 10.29228/jrp.292
Vancouver Canak Ipek T,Avci-Adali M,Ekentok C,Salva E,OZBAS TURAN S Chitosan-based delivery of CRISPR-Cas9 plasmid in breast cancer stem cells. Journal of research in pharmacy (online). 2023; 27(1): 86 - 96. 10.29228/jrp.292
IEEE Canak Ipek T,Avci-Adali M,Ekentok C,Salva E,OZBAS TURAN S "Chitosan-based delivery of CRISPR-Cas9 plasmid in breast cancer stem cells." Journal of research in pharmacy (online), 27, ss.86 - 96, 2023. 10.29228/jrp.292
ISNAD Canak Ipek, Tuba vd. "Chitosan-based delivery of CRISPR-Cas9 plasmid in breast cancer stem cells". Journal of research in pharmacy (online) 27/1 (2023), 86-96. https://doi.org/10.29228/jrp.292
Sayfa Oluşturulma Tarihi
24-04-2024 7:36

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