Investigation of Apoptotic Effect of Propofol, Dexmedetomidine and Medetomidine on Oocyte Cumulus Granulosa Cells in Rats
Yıl: 2023 Cilt: 31 Sayı: 1 Sayfa Aralığı: 50 - 54 Metin Dili: İngilizce DOI: 10.54875/jarss.2023.88942 İndeks Tarihi: 30-05-2023
Investigation of Apoptotic Effect of Propofol, Dexmedetomidine and Medetomidine on Oocyte Cumulus Granulosa Cells in Rats
Öz: Objective: We aimed to evaluate the potential apoptotic effects of propofol, dexmedetomidine and medetomidine that were used during oocyte retrieval on cumulus cells in rat ovulation induction model. Methods: After the ovulation induction model was created, rats were received dexmedetomidine in Group D, propofol in Group P, medetomidine in Group M. Oocytes collection was performed 10 minutes after the administration of study drugs. For Caspase-3 immunohistochemical evaluation, the staining level was scored in five randomly selected areas and the areas with the highest score were determined. Immunohistochemical staining scoring for each section was performed using a scoring algorithm called H-score. Results: Caspase-3 expression in cumulus cells was found to be lowest in Group D and highest in Group P. The mean caspase-3 H-score was lower in Group D than that of Group P and M (p<0.001), and in Group M than that of Group P (p<0.001). Conclusion: Our results demonstrate that dexmedetomidine and medetomidine exhibit less apoptotic effects in terms of caspase-3 activity in oocyte cumulus cells than propofol in a rat ovulation induction model.
Anahtar Kelime: Propofol, Deksmedetomidin ve Medetomidinin Sıçanlarda Oosit Kümülüs Granulosa Hücreleri Üzerine Apoptotik Etkilerinin Araştırılması
Öz: Amaç: Oosit toplama işlemi sırasında kullanılan propofol, deks- medetomidin ve medetomidinin kümülüs hücreleri üzerindeki po- tansiyel apoptotik etkilerini rat ovulasyon indüksiyon modelinde değerlendirmeyi amaçladık. Yöntem: Ovulasyon indüksiyon modeli oluşturulduktan sonra, sı- çanlara Grup D’de deksmedetomidin, Grup P’de propofol, Grup M’de medetomidin verildi. Çalışma ilaçlarının uygulanmasından 10 dakika sonra oosit toplama işlemi yapıldı. Kaspaz-3 immunohis- tokimyasal değerlendirmede rastgele seçilen beş alanda boyanma düzeyi skorlandı ve en yüksek skora sahip alanlar belirlendi. Her bölüm için immünohistokimyasal boyama puanlaması, H-skoru adı verilen bir puanlama algoritması kullanılarak yapıldı. Bulgular: Kümülüs hücrelerinde kaspaz-3 ekspresyonu Grup D’de en düşük, Grup P’de en yüksek bulundu. Ortalama kaspaz-3 H-sko- ru Grup D’de Grup P ve M’ye göre daha düşüktü (p<0,001) ve Grup M’de Grup P’den daha düşük bulundu (p<0,001). Sonuç: Sonuçlarımız deksmedetomidin ve medetomidinin sıçan ovulasyon indüksiyon modelinde oosit kümülüs hücrelerinde kas- paz-3 aktivitesi açısından propofole göre daha az apoptotik etkisi olduğunu göstermektedir.
Anahtar Kelime: Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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| APA | Tas Tuna A, kocayiğit h, DEMİR G, BUDAK O, bostanci m, Çakıroğlu H, GÜNAYDIN D (2023). Investigation of Apoptotic Effect of Propofol, Dexmedetomidine and Medetomidine on Oocyte Cumulus Granulosa Cells in Rats. , 50 - 54. 10.54875/jarss.2023.88942 |
| Chicago | Tas Tuna Ayca,kocayiğit havva,DEMİR GÜRKAN,BUDAK OZCAN,bostanci mehmet suhha,Çakıroğlu Hüseyin,GÜNAYDIN DUDU BERRİN Investigation of Apoptotic Effect of Propofol, Dexmedetomidine and Medetomidine on Oocyte Cumulus Granulosa Cells in Rats. (2023): 50 - 54. 10.54875/jarss.2023.88942 |
| MLA | Tas Tuna Ayca,kocayiğit havva,DEMİR GÜRKAN,BUDAK OZCAN,bostanci mehmet suhha,Çakıroğlu Hüseyin,GÜNAYDIN DUDU BERRİN Investigation of Apoptotic Effect of Propofol, Dexmedetomidine and Medetomidine on Oocyte Cumulus Granulosa Cells in Rats. , 2023, ss.50 - 54. 10.54875/jarss.2023.88942 |
| AMA | Tas Tuna A,kocayiğit h,DEMİR G,BUDAK O,bostanci m,Çakıroğlu H,GÜNAYDIN D Investigation of Apoptotic Effect of Propofol, Dexmedetomidine and Medetomidine on Oocyte Cumulus Granulosa Cells in Rats. . 2023; 50 - 54. 10.54875/jarss.2023.88942 |
| Vancouver | Tas Tuna A,kocayiğit h,DEMİR G,BUDAK O,bostanci m,Çakıroğlu H,GÜNAYDIN D Investigation of Apoptotic Effect of Propofol, Dexmedetomidine and Medetomidine on Oocyte Cumulus Granulosa Cells in Rats. . 2023; 50 - 54. 10.54875/jarss.2023.88942 |
| IEEE | Tas Tuna A,kocayiğit h,DEMİR G,BUDAK O,bostanci m,Çakıroğlu H,GÜNAYDIN D "Investigation of Apoptotic Effect of Propofol, Dexmedetomidine and Medetomidine on Oocyte Cumulus Granulosa Cells in Rats." , ss.50 - 54, 2023. 10.54875/jarss.2023.88942 |
| ISNAD | Tas Tuna, Ayca vd. "Investigation of Apoptotic Effect of Propofol, Dexmedetomidine and Medetomidine on Oocyte Cumulus Granulosa Cells in Rats". (2023), 50-54. https://doi.org/10.54875/jarss.2023.88942 |
| APA | Tas Tuna A, kocayiğit h, DEMİR G, BUDAK O, bostanci m, Çakıroğlu H, GÜNAYDIN D (2023). Investigation of Apoptotic Effect of Propofol, Dexmedetomidine and Medetomidine on Oocyte Cumulus Granulosa Cells in Rats. Anestezi Dergisi, 31(1), 50 - 54. 10.54875/jarss.2023.88942 |
| Chicago | Tas Tuna Ayca,kocayiğit havva,DEMİR GÜRKAN,BUDAK OZCAN,bostanci mehmet suhha,Çakıroğlu Hüseyin,GÜNAYDIN DUDU BERRİN Investigation of Apoptotic Effect of Propofol, Dexmedetomidine and Medetomidine on Oocyte Cumulus Granulosa Cells in Rats. Anestezi Dergisi 31, no.1 (2023): 50 - 54. 10.54875/jarss.2023.88942 |
| MLA | Tas Tuna Ayca,kocayiğit havva,DEMİR GÜRKAN,BUDAK OZCAN,bostanci mehmet suhha,Çakıroğlu Hüseyin,GÜNAYDIN DUDU BERRİN Investigation of Apoptotic Effect of Propofol, Dexmedetomidine and Medetomidine on Oocyte Cumulus Granulosa Cells in Rats. Anestezi Dergisi, vol.31, no.1, 2023, ss.50 - 54. 10.54875/jarss.2023.88942 |
| AMA | Tas Tuna A,kocayiğit h,DEMİR G,BUDAK O,bostanci m,Çakıroğlu H,GÜNAYDIN D Investigation of Apoptotic Effect of Propofol, Dexmedetomidine and Medetomidine on Oocyte Cumulus Granulosa Cells in Rats. Anestezi Dergisi. 2023; 31(1): 50 - 54. 10.54875/jarss.2023.88942 |
| Vancouver | Tas Tuna A,kocayiğit h,DEMİR G,BUDAK O,bostanci m,Çakıroğlu H,GÜNAYDIN D Investigation of Apoptotic Effect of Propofol, Dexmedetomidine and Medetomidine on Oocyte Cumulus Granulosa Cells in Rats. Anestezi Dergisi. 2023; 31(1): 50 - 54. 10.54875/jarss.2023.88942 |
| IEEE | Tas Tuna A,kocayiğit h,DEMİR G,BUDAK O,bostanci m,Çakıroğlu H,GÜNAYDIN D "Investigation of Apoptotic Effect of Propofol, Dexmedetomidine and Medetomidine on Oocyte Cumulus Granulosa Cells in Rats." Anestezi Dergisi, 31, ss.50 - 54, 2023. 10.54875/jarss.2023.88942 |
| ISNAD | Tas Tuna, Ayca vd. "Investigation of Apoptotic Effect of Propofol, Dexmedetomidine and Medetomidine on Oocyte Cumulus Granulosa Cells in Rats". Anestezi Dergisi 31/1 (2023), 50-54. https://doi.org/10.54875/jarss.2023.88942 |
