Evaluation of Vitamin D binding protein and 25-hydroxy Vitamin D metabolites in COVID-19 patients

Karakukcu, Cigdem; Saracoglu, Hatice; Çelik, İlhami; görkem, aysun; MUTLU SARIGUZEL, Fatma; Gülbahar, Özlem; Elibol, Ayça; Eryilmaz-Eren, Esma

doi:10.14744/ijmb.2023.54775

Evaluation of Vitamin D binding protein and 25-hydroxy Vitamin D metabolites in COVID-19 patients

Çiğdem KARAKÜKCÜ, (Department of Biochemistry, Erciyes University Faculty of Medicine, Kayseri, Türkiye)

Ayça Elibol, (Department of Internal Medicine, University of Health Science, Kayseri City Training and Research Hospital, Kayseri, Türkiye)

Esma EREN, (Department of Infectious Disease, University of Health Science, Kayseri City Training and Research Hospital, Kayseri, Türkiye)

Hatice SARAÇOĞLU, (Department of Biochemistry, Erciyes University Faculty of Medicine, Kayseri, Türkiye)

Fatma Mutlu SARIGÜZEL, (Department of Microbiology, Erciyes University Faculty of Medicine, Kayseri, Türkiye)

Aysun GÖRKEM, (Department of Microbiology, University of Health Science, Kayseri City Training and Research Hospital, Kayseri, Türkiye)

Özlem GÜLBAHAR, (Department of Biochemistry, Gazi University Faculty of Medicine, Ankara, Türkiye)

İhami ÇELİK (Department of Infectious Disease, University of Health Science, Kayseri City Training and Research Hospital, Kayseri, Türkiye)

International Journal of Medical Biochemistry

6 0

Yıl: 2023 Cilt: 6 Sayı: 2 Sayfa Aralığı: 69 - 74 Metin Dili: İngilizce DOI: 10.14744/ijmb.2023.54775 İndeks Tarihi: 31-05-2023

Evaluation of Vitamin D binding protein and 25-hydroxy Vitamin D metabolites in COVID-19 patients

Öz:

Objectives: The immunomodulatory roles of Vitamin D and Vitamin D binding protein (VDBP) are in interest with in- cidence or outcome of coronavirus disease-2019 (COVID-19). This study aimed to investigate the association between the severity of COVID-19 with VDBP, total 25-hydroxy Vitamin D (25(OH)D), and its metabolites free Vitamin D (VDfree) and bioavailable Vitamin D (VDbio). Methods: Study group consisted of 68 COVID-19 patients and 20 healthy subjects. Patients were subgrouped as as- ymptotic, mild/moderately pneumonia, or severe pneumonia. Plasma total 25(OH)D was quantitated by liquid chro- matography with mass spectrometry and serum VDBP by a polyclonal sandwich enzyme immunoassay. In addition, routinely used laboratory parameters in follow-up were recorded. VDfree and VDbio were calculated using total 25(OH)D, VDBP, and albumin levels. Results: Plasma total 25(OH)D (13.3±5.7 vs. 30.3±13.3 ng/dL), VDfree (2.18 [1.52–3.44] vs. 4.34 [3.74–6.48] pg/mL), and VDbio (1.86 [1.09–2.81] vs. 4.28 [3.45–6.34] nmol/L) levels were lower in COVID-19 patients (p<0.001). Despite the in- significance of 25(OH)D and metabolites between COVID-19 severity subgroups, serum VDBP was highest in mild/ moderately pneumonia (601.8±278.6 ng/mL) and lowest in severe pneumonia (427.9±147.2 ng/mL) (p<0.001). In addi- tion, VDBP was positively correlated with lymphocyte counts (B:87.9, r2=0.068, p=0.031) and negatively correlated with D-Dimer levels (B:−0.024, r2=0.081, p=0.032). Conclusion: COVID-19 patients have lower plasma 25(OH)D levels and lower 25(OH)D metabolites VDfree, VDbio which are physiologically active. In addition, serum VDBP concentrations significantly decrease in critically ill patients which needs further studies to be associated in the etiopathogenesis of the disease severity.

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APA	Karakukcu C, Elibol A, Eryilmaz-Eren E, Saracoglu H, MUTLU SARIGUZEL F, görkem a, Gülbahar Ö, Çelik İ (2023). Evaluation of Vitamin D binding protein and 25-hydroxy Vitamin D metabolites in COVID-19 patients. , 69 - 74. 10.14744/ijmb.2023.54775
Chicago	Karakukcu Cigdem,Elibol Ayça,Eryilmaz-Eren Esma,Saracoglu Hatice,MUTLU SARIGUZEL Fatma,görkem aysun,Gülbahar Özlem,Çelik İlhami Evaluation of Vitamin D binding protein and 25-hydroxy Vitamin D metabolites in COVID-19 patients. (2023): 69 - 74. 10.14744/ijmb.2023.54775
MLA	Karakukcu Cigdem,Elibol Ayça,Eryilmaz-Eren Esma,Saracoglu Hatice,MUTLU SARIGUZEL Fatma,görkem aysun,Gülbahar Özlem,Çelik İlhami Evaluation of Vitamin D binding protein and 25-hydroxy Vitamin D metabolites in COVID-19 patients. , 2023, ss.69 - 74. 10.14744/ijmb.2023.54775
AMA	Karakukcu C,Elibol A,Eryilmaz-Eren E,Saracoglu H,MUTLU SARIGUZEL F,görkem a,Gülbahar Ö,Çelik İ Evaluation of Vitamin D binding protein and 25-hydroxy Vitamin D metabolites in COVID-19 patients. . 2023; 69 - 74. 10.14744/ijmb.2023.54775
Vancouver	Karakukcu C,Elibol A,Eryilmaz-Eren E,Saracoglu H,MUTLU SARIGUZEL F,görkem a,Gülbahar Ö,Çelik İ Evaluation of Vitamin D binding protein and 25-hydroxy Vitamin D metabolites in COVID-19 patients. . 2023; 69 - 74. 10.14744/ijmb.2023.54775
IEEE	Karakukcu C,Elibol A,Eryilmaz-Eren E,Saracoglu H,MUTLU SARIGUZEL F,görkem a,Gülbahar Ö,Çelik İ "Evaluation of Vitamin D binding protein and 25-hydroxy Vitamin D metabolites in COVID-19 patients." , ss.69 - 74, 2023. 10.14744/ijmb.2023.54775
ISNAD	Karakukcu, Cigdem vd. "Evaluation of Vitamin D binding protein and 25-hydroxy Vitamin D metabolites in COVID-19 patients". (2023), 69-74. https://doi.org/10.14744/ijmb.2023.54775

APA	Karakukcu C, Elibol A, Eryilmaz-Eren E, Saracoglu H, MUTLU SARIGUZEL F, görkem a, Gülbahar Ö, Çelik İ (2023). Evaluation of Vitamin D binding protein and 25-hydroxy Vitamin D metabolites in COVID-19 patients. International Journal of Medical Biochemistry, 6(2), 69 - 74. 10.14744/ijmb.2023.54775
Chicago	Karakukcu Cigdem,Elibol Ayça,Eryilmaz-Eren Esma,Saracoglu Hatice,MUTLU SARIGUZEL Fatma,görkem aysun,Gülbahar Özlem,Çelik İlhami Evaluation of Vitamin D binding protein and 25-hydroxy Vitamin D metabolites in COVID-19 patients. International Journal of Medical Biochemistry 6, no.2 (2023): 69 - 74. 10.14744/ijmb.2023.54775
MLA	Karakukcu Cigdem,Elibol Ayça,Eryilmaz-Eren Esma,Saracoglu Hatice,MUTLU SARIGUZEL Fatma,görkem aysun,Gülbahar Özlem,Çelik İlhami Evaluation of Vitamin D binding protein and 25-hydroxy Vitamin D metabolites in COVID-19 patients. International Journal of Medical Biochemistry, vol.6, no.2, 2023, ss.69 - 74. 10.14744/ijmb.2023.54775
AMA	Karakukcu C,Elibol A,Eryilmaz-Eren E,Saracoglu H,MUTLU SARIGUZEL F,görkem a,Gülbahar Ö,Çelik İ Evaluation of Vitamin D binding protein and 25-hydroxy Vitamin D metabolites in COVID-19 patients. International Journal of Medical Biochemistry. 2023; 6(2): 69 - 74. 10.14744/ijmb.2023.54775
Vancouver	Karakukcu C,Elibol A,Eryilmaz-Eren E,Saracoglu H,MUTLU SARIGUZEL F,görkem a,Gülbahar Ö,Çelik İ Evaluation of Vitamin D binding protein and 25-hydroxy Vitamin D metabolites in COVID-19 patients. International Journal of Medical Biochemistry. 2023; 6(2): 69 - 74. 10.14744/ijmb.2023.54775
IEEE	Karakukcu C,Elibol A,Eryilmaz-Eren E,Saracoglu H,MUTLU SARIGUZEL F,görkem a,Gülbahar Ö,Çelik İ "Evaluation of Vitamin D binding protein and 25-hydroxy Vitamin D metabolites in COVID-19 patients." International Journal of Medical Biochemistry, 6, ss.69 - 74, 2023. 10.14744/ijmb.2023.54775
ISNAD	Karakukcu, Cigdem vd. "Evaluation of Vitamin D binding protein and 25-hydroxy Vitamin D metabolites in COVID-19 patients". International Journal of Medical Biochemistry 6/2 (2023), 69-74. https://doi.org/10.14744/ijmb.2023.54775