TY  - JOUR
TI  - Clinical, electrophysiological, and histomorphological effects of local coenzyme  Q10 and vitamin E use in a rat model of peripheral nerve injury
AB  - Objective: This study aimed to investigate the clinical, electrophysiological, and histomorphological effects of local use of coenzyme  Q10 and vitamin E combination in a rat model of peripheral nerve injury. Methods: Forty adult female Wistar-Albino rats weighing 250-350 g were kept in a room with a temperature of 20-22°C and a light/dark  cycle of 12 hours. They had free access to food and water. The right sciatic nerves of 40 rats were transected and repaired. Subjects were  divided into 4 groups: controls (control-4 weeks and control-8 weeks) and treatments (treatment-4 weeks and treatment-8 weeks). A  combination of coenzyme Q10 and vitamin E was applied to the repair site by a catheter placed subcutaneously in the treatment group.  Only transection-repair was done in the control group. All groups were divided into 2 subgroups for histomorphological, clinical, and  electrophysiological experiments because of concerns about possible interference with histomorphological preparation (5 rats in each  group). The experiment results were examined by the thermal plantar test, action potential and latency time measurements, and electron  microscopy at the end of 4 and 8 weeks. The intact group was studied as the uninterrupted 10 left sciatic nerves of control for 4 weeks. Results: The mean thermal plantar test results of the intact group were better than those of the control groups (P < .05). However, there  was no significant difference between the intact and treatment groups. In the histomorphological examination, the number of myelinated  axons increased significantly, and the myelin structure was closer to that of the intact group, especially when the treatment-8 group was  compared with the control groups (control-4: P < .0001, control-8: P < .01). Conclusion: Local use of coenzyme Q10 and vitamin E seems useful in the experimental rat sciatic nerve transection-repair model.
AU  - Ocal, Isil
AU  - Şaker, Dilek
AU  - Mirioğlu, Akif
AU  - Olke, Hakki Can
AU  - Bicer, Omer
AU  - ozkan, cenk
DO  - 10.5152/j.aott.2023.22121
PY  - 2023
JO  - Acta Orthopaedica et Traumatologica Turcica
VL  - 57
IS  - 1
SN  - 1017-995X
SP  - 23
EP  - 29
DB  - TRDizin
UR  - http://search/yayin/detay/1177912
ER  -