Evaluation of Cytotoxicity and Antibacterial Effect of Different Types of Mineral Trioxide Aggregate

KUSOGLU, SEDA; Unlu, Ozge; EGIL, EDIBE; Demirci, Mehmet

doi:10.4274/meandros.galenos.2021.54036

Evaluation of Cytotoxicity and Antibacterial Effect of Different Types of Mineral Trioxide Aggregate

Özge ÜNLÜ, (İstanbul Atlas Üniversitesi, Tıp Fakültesi, Tıbbi Mikrobiyoloji Anabilim Dalı, İstanbul, Türkiye)

Edibe EGİL, (İstanbul Aydın Üniversitesi, Diş Hekimliği Fakültesi, Çocuk Diş Hekimliği Anabilim Dalı, İstanbul, Türkiye)

Mehmet DEMİRCİ, (Kırklareli Üniversitesi, Tıp Fakültesi, Tıbbi Mikrobiyoloji Anabilim Dalı, Kırklareli, Türkiye)

Seda KUŞOĞLU GÜLTEKİN (Üsküdar Üniversitesi, Mühendislik ve Doğa Bilimleri Fakültesi, Moleküler Biyoloji ve Genetik Bölümü, İstanbul, Türkiye)

Meandros Medical And Dental Journal

9 2

Yıl: 2023 Cilt: 24 Sayı: 1 Sayfa Aralığı: 13 - 18 Metin Dili: İngilizce DOI: 10.4274/meandros.galenos.2021.54036 İndeks Tarihi: 01-06-2023

Evaluation of Cytotoxicity and Antibacterial Effect of Different Types of Mineral Trioxide Aggregate

Öz:

Objective: Mineral trioxide aggregate (MTA) has been used as a filling material in endodontic procedures over decades. The newer formulations of MTA were launched in the dental market and their cytotoxic, proliferative and antimicrobial effects need to be revealed. This study compared the possible cytotoxic and proliferative effects on fibroblasts and the antimicrobial activity against Streptococcus mutans, Lactobacillus acidophilus, and Enterococcus faecalis of four different MTAs in the dental market. Materials and Methods: Cytotoxicity assay was performed on 3T3 fibroblast cell lines were determined using yellow tetrazolium 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, while the antimicrobial activity was tested with the broth microdilution method. Results: RetroMTA, AngelusMTA, and NeoMTA demonstrated a proliferative effect on 3T3 cells, suggesting induction in tissue repair. Moreover, NeoMTA showed the highest antimicrobial activity against all strains tested. Conclusion: According to our study, NeoMTA and RetroMTA may be recommended for clinical applications in comparison with the conventional AngelusMTA.

Anahtar Kelime:

Farklı Mineral Trioksit Agregatların Sitotoksisite ve Antibakteriyel Etkilerinin Değerlendirilmesi

Öz:

Amaç: Mineral trioksit agregat (MTA), endodontik prosedürlerde dolgu malzemesi olarak yıllardır kullanılmaktadır. MTA’nın sitotoksik, proliferatif ve antimikrobiyal etkilerinin yeni formülasyonlarda test edilmesi ihtiyacı oluşturmaktadır. Bu çalışma, fibroblastlar üzerindeki olası sitotoksik ve proliferatif etkileri ile dört farklı MTA’nın Streptococcus mutans, Lactobacillus acidophilus ve Enterococcus faecalis üzerindeki antimikrobiyal aktivitesini karşılaştırmayı amaçlamaktadır. Gereç ve Yöntemler: 3T3 fibroblast hücre hatları üzerinde sitotoksisite analizi sarı tetrazolyum 3-(4,5-dimetiltiyazol-2-il)-2,5- difeniltetrazolium bromid kullanılarak, antimikrobiyal aktivite ise sıvı mikrodilüsyon yöntemi ile test edildi. Bulgular: RetroMTA, AngelusMTA ve NeoMTA, 3T3 hücreleri üzerinde proliferatif etki gösterdiği gözlemlenmiştir. Antibakteriyel etki açısından incelendiği zaman NeoMTA en yüksek etkiyi göstermiştir. Sonuç: Çalışmamıza göre NeoMTA ve RetroMTA’nın klinik uygulamalarda klasik AngelusMTA’ya alternatif olarak önerilebileceği düşünülmektedir.

Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	Unlu O, EGIL E, Demirci M, KUSOGLU S (2023). Evaluation of Cytotoxicity and Antibacterial Effect of Different Types of Mineral Trioxide Aggregate. , 13 - 18. 10.4274/meandros.galenos.2021.54036
Chicago	Unlu Ozge,EGIL EDIBE,Demirci Mehmet,KUSOGLU SEDA Evaluation of Cytotoxicity and Antibacterial Effect of Different Types of Mineral Trioxide Aggregate. (2023): 13 - 18. 10.4274/meandros.galenos.2021.54036
MLA	Unlu Ozge,EGIL EDIBE,Demirci Mehmet,KUSOGLU SEDA Evaluation of Cytotoxicity and Antibacterial Effect of Different Types of Mineral Trioxide Aggregate. , 2023, ss.13 - 18. 10.4274/meandros.galenos.2021.54036
AMA	Unlu O,EGIL E,Demirci M,KUSOGLU S Evaluation of Cytotoxicity and Antibacterial Effect of Different Types of Mineral Trioxide Aggregate. . 2023; 13 - 18. 10.4274/meandros.galenos.2021.54036
Vancouver	Unlu O,EGIL E,Demirci M,KUSOGLU S Evaluation of Cytotoxicity and Antibacterial Effect of Different Types of Mineral Trioxide Aggregate. . 2023; 13 - 18. 10.4274/meandros.galenos.2021.54036
IEEE	Unlu O,EGIL E,Demirci M,KUSOGLU S "Evaluation of Cytotoxicity and Antibacterial Effect of Different Types of Mineral Trioxide Aggregate." , ss.13 - 18, 2023. 10.4274/meandros.galenos.2021.54036
ISNAD	Unlu, Ozge vd. "Evaluation of Cytotoxicity and Antibacterial Effect of Different Types of Mineral Trioxide Aggregate". (2023), 13-18. https://doi.org/10.4274/meandros.galenos.2021.54036

APA	Unlu O, EGIL E, Demirci M, KUSOGLU S (2023). Evaluation of Cytotoxicity and Antibacterial Effect of Different Types of Mineral Trioxide Aggregate. Meandros Medical And Dental Journal, 24(1), 13 - 18. 10.4274/meandros.galenos.2021.54036
Chicago	Unlu Ozge,EGIL EDIBE,Demirci Mehmet,KUSOGLU SEDA Evaluation of Cytotoxicity and Antibacterial Effect of Different Types of Mineral Trioxide Aggregate. Meandros Medical And Dental Journal 24, no.1 (2023): 13 - 18. 10.4274/meandros.galenos.2021.54036
MLA	Unlu Ozge,EGIL EDIBE,Demirci Mehmet,KUSOGLU SEDA Evaluation of Cytotoxicity and Antibacterial Effect of Different Types of Mineral Trioxide Aggregate. Meandros Medical And Dental Journal, vol.24, no.1, 2023, ss.13 - 18. 10.4274/meandros.galenos.2021.54036
AMA	Unlu O,EGIL E,Demirci M,KUSOGLU S Evaluation of Cytotoxicity and Antibacterial Effect of Different Types of Mineral Trioxide Aggregate. Meandros Medical And Dental Journal. 2023; 24(1): 13 - 18. 10.4274/meandros.galenos.2021.54036
Vancouver	Unlu O,EGIL E,Demirci M,KUSOGLU S Evaluation of Cytotoxicity and Antibacterial Effect of Different Types of Mineral Trioxide Aggregate. Meandros Medical And Dental Journal. 2023; 24(1): 13 - 18. 10.4274/meandros.galenos.2021.54036
IEEE	Unlu O,EGIL E,Demirci M,KUSOGLU S "Evaluation of Cytotoxicity and Antibacterial Effect of Different Types of Mineral Trioxide Aggregate." Meandros Medical And Dental Journal, 24, ss.13 - 18, 2023. 10.4274/meandros.galenos.2021.54036
ISNAD	Unlu, Ozge vd. "Evaluation of Cytotoxicity and Antibacterial Effect of Different Types of Mineral Trioxide Aggregate". Meandros Medical And Dental Journal 24/1 (2023), 13-18. https://doi.org/10.4274/meandros.galenos.2021.54036