Sweet cherry, which affects consumers’ preferences with its aroma, taste and sensory properties, is a significant fruit species for human health with its bioactive compounds such as organic acids, vitamins and anthocyanins. However, its post-harvest shelf life is very short due to its sensitive fruit structure. Thus, the significant economic losses occur. In the study, effects of pre-harvest Aminoethoxyvinylglycine (AVG), Salicylic acid (SA) and plant growth promoting rhizobacteria (PGPR) applications on fruit quality characteristics and biochemical content in sweet cherry were determined. SA and PGPR treatments increased fruit size, but AVG decreased (If the fruit weight is 4.51 in the control application, 4.49 in the SA application and 4.10 in the PGPR, how can it be said that SA and PGPR increase the fruit weight). The fruits treated with SA, AVG and PGPR had higher fruit firmness values than the control’-fruits. Salisylic acid treatmens did not affect the rate of soluble solids content(SSC) in fruit, SSC rate was higher in PGPR treated-fruit, but AVG decreased the SSC. Titratable acidity (TA) was lower in PGPR treated-fruit, but was higher in the AVG and SA treated-fruit. Fruits treated with the SA and AVG had higher fruit color values than control fruits, while the lowest color values was obtained with PGPR treatment. In general; PGPR, SA and AVG were effective in the concentration of organic acids, but their effects varied depending on the organic acid compounds. As a result, AVG and SA delayed fruit ripening and fruit softening while PGPR, AVG and SA improved fruit quality (It is not clear how this idea was reached). These applications can be considered as a promising method for improving fruit quality at harvest and maintaining post-harvest fruit quality of sweet cherry.