Introduction: Although nutrition is very effective on mortality in critically ill children, patients cannot be fed adequately. There is no suitable biomarker for enteral nutrition monitoring and management. The aim of this study; To investigate the usability of nefsatin-1 for nutritional monitoring and management in critically ill children. Methods: In the January-September 2019 period, 35 critically ill children who were hospitalized in the tertiary pediatric intensive care unit without any signs of inflammation were included in the study. Nesfatin-1, vitamin D level, prealbumin, albumin was evaluated for the study. Results: Thirty-five patients who were admitted to the pediatric intensive care unit and whose hospitalization reasons had regressed were evaluated. The mean nesfatin-1 values of the patients were measured as 9.95±0.78 ng/mL (4.25-18.93 ng/mL). There was a negative and low (r=-0.214) relationship between target calorie intake and nesfatin-1, a positive and low (r=0.172) relationship with prealbumin, and a positive and high relationship with vitamin D (r=0.529). Similar relationships were determined between the weight change ratio and nesfatin-1 (r=-0.266), prealbumin (r=0.154) and vitamin D (r=0.337). Conclusion: Based on the study findings, it was concluded that serum nefsatin-1, albumin and prealbumin are not appropriate indicators for nutritional monitoring and management in critically ill children.