An evaluation of the effects on the ovaries of hyperbaric oxygen therapy in a rat model of premature ovarian failure created with cyclophosphamide
Yıl: 2023 Cilt: 20 Sayı: 1 Sayfa Aralığı: 46 - 52 Metin Dili: İngilizce DOI: 10.4274/tjod.galenos.2023.47817 İndeks Tarihi: 03-06-2023
An evaluation of the effects on the ovaries of hyperbaric oxygen therapy in a rat model of premature ovarian failure created with cyclophosphamide
Öz: Objective: To evaluate hyperbaric oxygen therapy (HBO) based on ovarian histology, total antioxidant status (TAS), total oxidant status (TOS), and anti- müllerian hormone (AMH), in the ovarian insufiency (POI) model created with cyclophosphamide (CYP). Materials and Methods: The rats were separated into 3 groups of the control group (n=6), the CYP group (n=6), and the CYP+HBO group (n=6). The rats in the CYP group and the CYP+HBO group were injected intraperitoneally with 200 mg/kg CYP on day 1, followed by 8 mg/kg/day for 14 days to create POI. From the 15th day onwards, the rats in the CYP+HBO group were placed in a hyperbaric cabin and exposed to 100% oxygen at 2.4 atm pressure for one h, and were then returned to their cages at the end of the hour. Results: A statistically significant decrease was determined in the primordial and primary follicle counts in the CYP group compared with the control group (p<0.05). In the CYP+HBO group, a statistically significant increase was determined in the primordial and primary follicle counts (p<0.05). The serum AMH levels were seen to be significantly decreased in the CYP group compared with both the control group and the CYP+HBO groups. The HBO was seen to decrease TOS and increase TAS. Conclusion: HBO could be an alternative treatment to minimize the effect of ovarian follicle loss caused by CYP, which is used for treating tumors that commonly occur in young females of reproductive age.
Anahtar Kelime: Siklofosfamid ile oluşturulmuş erken yumurtalık yetmezliği olan bir sıçan modelinde hiperbarik oksijen tedavisinin yumurtalıklar üzerindeki etkilerinin değerlendirilmesi
Öz: Amaç: Bu çalışmanın amacı siklofosfamid (CYP) ile oluşturulan over yetmezliği (POI) modelinde hiperbarik oksijen tedavisini (HBO) over histolojisi, total antioksidan durumu (TAS), total oksidan durumu (TOS) ve anti-müllerian hormon (AMH) bazında değerlendirmektir. Gereç ve Yöntemler: Ratlar kontrol grubu (n=6), CYP grubu (n=6) ve CYP+HBO grubu (n=6) olmak üzere 3 gruba ayrıldı. CYP grubu ve CYP+HBO grubundaki ratlara 1. gün 200 mg/kg CYP, ardından 14 gün boyunca POI oluşturmak için 8 mg/kg/gün intraperitoneal olarak enjekte edildi. 15. günden itibaren CYP+HBO grubundaki ratlar hiperbarik kabine alınarak 1 saat 2,4 atm basınçta %100 oksijene maruz bırakıldı ve 1 saatin sonunda ratlar tekrar kafeslerine alındı. Bulgular: CYP grubundaki primordial ve primer folikül sayılarında kontrol grubuna göre istatistiksel olarak anlamlı azalma saptandı (p<0,05). CYP+HBO grubunda primordial ve primer folikül sayılarında istatistiksel olarak anlamlı artış saptandı (p<0,05). Serum AMH düzeylerinin CYP grubunda hem kontrol grubu hem de CYP+HBO grubuna göre anlamlı olarak düştüğü görüldü. HBO’nun TOS’yi azalttığı ve TAS’yi artırdığı görüldü. Sonuç: Üreme çağındaki genç kadınlarda sık görülen tümörlerin tedavisinde kullanılan CYP’nin neden olduğu over folikül kaybının etkisini en aza indirmek için HBO alternatif bir tedavi olabilir.
Anahtar Kelime: Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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| APA | çağlı f, Baktir M, Dolanbay M, Balcıoğlu E, Cumaoğlu A, ERMİŞ M, KARAMAN E, YAY b, Aygen E (2023). An evaluation of the effects on the ovaries of hyperbaric oxygen therapy in a rat model of premature ovarian failure created with cyclophosphamide. , 46 - 52. 10.4274/tjod.galenos.2023.47817 |
| Chicago | çağlı fulya,Baktir Mehmet Akif,Dolanbay Mehmet,Balcıoğlu Esra,Cumaoğlu Ahmet,ERMİŞ Mustafa,KARAMAN Enes,YAY betül,Aygen Ercan mustafa An evaluation of the effects on the ovaries of hyperbaric oxygen therapy in a rat model of premature ovarian failure created with cyclophosphamide. (2023): 46 - 52. 10.4274/tjod.galenos.2023.47817 |
| MLA | çağlı fulya,Baktir Mehmet Akif,Dolanbay Mehmet,Balcıoğlu Esra,Cumaoğlu Ahmet,ERMİŞ Mustafa,KARAMAN Enes,YAY betül,Aygen Ercan mustafa An evaluation of the effects on the ovaries of hyperbaric oxygen therapy in a rat model of premature ovarian failure created with cyclophosphamide. , 2023, ss.46 - 52. 10.4274/tjod.galenos.2023.47817 |
| AMA | çağlı f,Baktir M,Dolanbay M,Balcıoğlu E,Cumaoğlu A,ERMİŞ M,KARAMAN E,YAY b,Aygen E An evaluation of the effects on the ovaries of hyperbaric oxygen therapy in a rat model of premature ovarian failure created with cyclophosphamide. . 2023; 46 - 52. 10.4274/tjod.galenos.2023.47817 |
| Vancouver | çağlı f,Baktir M,Dolanbay M,Balcıoğlu E,Cumaoğlu A,ERMİŞ M,KARAMAN E,YAY b,Aygen E An evaluation of the effects on the ovaries of hyperbaric oxygen therapy in a rat model of premature ovarian failure created with cyclophosphamide. . 2023; 46 - 52. 10.4274/tjod.galenos.2023.47817 |
| IEEE | çağlı f,Baktir M,Dolanbay M,Balcıoğlu E,Cumaoğlu A,ERMİŞ M,KARAMAN E,YAY b,Aygen E "An evaluation of the effects on the ovaries of hyperbaric oxygen therapy in a rat model of premature ovarian failure created with cyclophosphamide." , ss.46 - 52, 2023. 10.4274/tjod.galenos.2023.47817 |
| ISNAD | çağlı, fulya vd. "An evaluation of the effects on the ovaries of hyperbaric oxygen therapy in a rat model of premature ovarian failure created with cyclophosphamide". (2023), 46-52. https://doi.org/10.4274/tjod.galenos.2023.47817 |
| APA | çağlı f, Baktir M, Dolanbay M, Balcıoğlu E, Cumaoğlu A, ERMİŞ M, KARAMAN E, YAY b, Aygen E (2023). An evaluation of the effects on the ovaries of hyperbaric oxygen therapy in a rat model of premature ovarian failure created with cyclophosphamide. Turkish Journal of Obstetrics and Gynecology, 20(1), 46 - 52. 10.4274/tjod.galenos.2023.47817 |
| Chicago | çağlı fulya,Baktir Mehmet Akif,Dolanbay Mehmet,Balcıoğlu Esra,Cumaoğlu Ahmet,ERMİŞ Mustafa,KARAMAN Enes,YAY betül,Aygen Ercan mustafa An evaluation of the effects on the ovaries of hyperbaric oxygen therapy in a rat model of premature ovarian failure created with cyclophosphamide. Turkish Journal of Obstetrics and Gynecology 20, no.1 (2023): 46 - 52. 10.4274/tjod.galenos.2023.47817 |
| MLA | çağlı fulya,Baktir Mehmet Akif,Dolanbay Mehmet,Balcıoğlu Esra,Cumaoğlu Ahmet,ERMİŞ Mustafa,KARAMAN Enes,YAY betül,Aygen Ercan mustafa An evaluation of the effects on the ovaries of hyperbaric oxygen therapy in a rat model of premature ovarian failure created with cyclophosphamide. Turkish Journal of Obstetrics and Gynecology, vol.20, no.1, 2023, ss.46 - 52. 10.4274/tjod.galenos.2023.47817 |
| AMA | çağlı f,Baktir M,Dolanbay M,Balcıoğlu E,Cumaoğlu A,ERMİŞ M,KARAMAN E,YAY b,Aygen E An evaluation of the effects on the ovaries of hyperbaric oxygen therapy in a rat model of premature ovarian failure created with cyclophosphamide. Turkish Journal of Obstetrics and Gynecology. 2023; 20(1): 46 - 52. 10.4274/tjod.galenos.2023.47817 |
| Vancouver | çağlı f,Baktir M,Dolanbay M,Balcıoğlu E,Cumaoğlu A,ERMİŞ M,KARAMAN E,YAY b,Aygen E An evaluation of the effects on the ovaries of hyperbaric oxygen therapy in a rat model of premature ovarian failure created with cyclophosphamide. Turkish Journal of Obstetrics and Gynecology. 2023; 20(1): 46 - 52. 10.4274/tjod.galenos.2023.47817 |
| IEEE | çağlı f,Baktir M,Dolanbay M,Balcıoğlu E,Cumaoğlu A,ERMİŞ M,KARAMAN E,YAY b,Aygen E "An evaluation of the effects on the ovaries of hyperbaric oxygen therapy in a rat model of premature ovarian failure created with cyclophosphamide." Turkish Journal of Obstetrics and Gynecology, 20, ss.46 - 52, 2023. 10.4274/tjod.galenos.2023.47817 |
| ISNAD | çağlı, fulya vd. "An evaluation of the effects on the ovaries of hyperbaric oxygen therapy in a rat model of premature ovarian failure created with cyclophosphamide". Turkish Journal of Obstetrics and Gynecology 20/1 (2023), 46-52. https://doi.org/10.4274/tjod.galenos.2023.47817 |
