TY  - JOUR
TI  - A Newly Developed and Fully Validated HPLC Method for  Simultenous Determination of in-situ Single Pass  Intestinal Permeability of Domperidone, Metoprolol  Tartarate and Phenol Red
AB  - One of the most popular methods for figuring out a drug’s intestinal permeability is the intestinal  perfusion (SPIP) approach. Metoprolol as tartrate and phenol red are frequently utilized as reference compounds in  perfusion tests to assess the permeability coefficient of the study’s focus component, Domperidone maleate. Our study’s  goal was to create and validate a reversed-phase liquid chromatographic technique for the simultaneous detection of  Domperidone maleate, metoprolol, and phenol red for use in intestinal perfusion studies. The analysis was performed  on a C18 column 150 × 1.5 mm, 5.0 µm using a mobile phase consisting of methanol: 20 mM potassium dihydrogen  phosphate buffer 43:57, v/v; pH 3.1. The procedure was validated using the FDA’s standards for stability, linearity,  precision, and accuracy. All calibration curves were linear r2> 0.99. The system suitability parameters of the method are  the required limits all analytes. The in situ intestinal permeability experiments can easily be standardized with the help  of this newly created and proven approach.
AU  - ÖZCAN, Saniye
AU  - KAYNAK, Mustafa Sinan
AU  - ÇAĞLAR, Emre Şefik
AU  - ÜSTÜNDAĞ OKUR, Neslihan
DO  - 10.29228/jrp.392
PY  - 2023
JO  - Journal of research in pharmacy (online)
VL  - 27
IS  - 3
SN  - 2630-6344
SP  - 967
EP  - 979
DB  - TRDizin
UR  - http://search/yayin/detay/1179342
ER  -