The Role of Matrix Metalloproteinase As Biomarkers For Neural Tube Defect

Denizler Ebiri, Farika Nur; Taspinar, Filiz; taspinar, mehmet; AKYOL, MEHMET EDIP; yüksek, veysel; Tuncer, Oğuz; Arslan, Mehmet

doi:10.5505/ejm.2023.24356

The Role of Matrix Metalloproteinase As Biomarkers For Neural Tube Defect

Mehmet Edip AKYOL, (Yüzüncü Yıl Üniversitesi Tıp Fakültesi Nöroşirürji Anabilim Dalı, Van, Türkiye)

Farika Nur Denizler Ebiri, (Yüzüncü Yıl Üniversitesi, Üniversite Tıp Fakültesi, Sinirbilim Anabilim Dalı, Van, Türkiye)

Filiz TAŞPINAR, (Aksaray Üniversitesi Tıp Fakültesi Fizyoloji Anabilim Dalı, Konya, Türkiye)

Veysel YÜKSEK, (Yüzüncü Yıl Üniversitesi Veteriner Fakültesi, Van, Türkiye)

Oğuz TUNCER, (Yüzüncü Yıl Üniversitesi Tıp Fakültesi Neonatoloji Anabilim Dalı, Van, Türkiye)

Mehmet ARSLAN, (Yüzüncü Yıl Üniversitesi Tıp Fakültesi Nöroşirürji Anabilim Dalı, Van, Türkiye)

MEHMET TAŞPINAR (Aksaray Üniversitesi Tıp Fakültesi Tıbbi Genetik Anabilim Dalı, Konya, Türkiye)

Eastern Journal of Medicine

6 3

Yıl: 2023 Cilt: 28 Sayı: 1 Sayfa Aralığı: 195 - 202 Metin Dili: İngilizce DOI: 10.5505/ejm.2023.24356 İndeks Tarihi: 06-06-2023

The Role of Matrix Metalloproteinase As Biomarkers For Neural Tube Defect

Öz:

Neural Tube Defect (NTD) is one of the most common congenital malformations. It is crucial to determine the prognostic, predictive, or therapeutic genetic factors for prevent ing NTD. The formation of the extracellular matrix (ECM) plays an essential role in migrating neural crest cells. Matrix metalloproteinases (MMPs) play a significant role in cell migration in ECM organization. The role of expressions and activation of MMP in NTD is unknown. This study aimed to investigate the roles of MMP-1, -2, and 9 gene expressions as biomarkers for NTD. Peripheral blood samples and NTD tissues were collected from 40 newborn babies diagnosed with NTD, which were also divided into subgroups based on pathology, and peripheral blood samples from only 20 healthy babies were taken for control. After total RNA isolation from blood and tissues, MMP -1, -2, -9 gene expressions were analyzed by Quantitative Real-Time PCR (RT-PCR). There was no difference between the control group and the NTD group in terms of MMP expressions in blood samples (p>0.05). A statistically significantly higher MMP-1 expression was found in Meningocele and Myeloschisis than in Encephalocele (p=0.014). A significant difference was found between the tissue and blood samples of the Meningomyelocele patient group regarding MMP-9 expression (p=0.019). There was no significant relationship between Ca2+, B12, and Folate levels, NTD, and MMP genes expressions (p>0.05). Even though MMP genes were not different between control and NTD groups, they were found to vary between different subgroups and can serve as biomarkers.

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APA	AKYOL M, Denizler Ebiri F, Taspinar F, yüksek v, Tuncer O, Arslan M, taspinar m (2023). The Role of Matrix Metalloproteinase As Biomarkers For Neural Tube Defect. , 195 - 202. 10.5505/ejm.2023.24356
Chicago	AKYOL MEHMET EDIP,Denizler Ebiri Farika Nur,Taspinar Filiz,yüksek veysel,Tuncer Oğuz,Arslan Mehmet,taspinar mehmet The Role of Matrix Metalloproteinase As Biomarkers For Neural Tube Defect. (2023): 195 - 202. 10.5505/ejm.2023.24356
MLA	AKYOL MEHMET EDIP,Denizler Ebiri Farika Nur,Taspinar Filiz,yüksek veysel,Tuncer Oğuz,Arslan Mehmet,taspinar mehmet The Role of Matrix Metalloproteinase As Biomarkers For Neural Tube Defect. , 2023, ss.195 - 202. 10.5505/ejm.2023.24356
AMA	AKYOL M,Denizler Ebiri F,Taspinar F,yüksek v,Tuncer O,Arslan M,taspinar m The Role of Matrix Metalloproteinase As Biomarkers For Neural Tube Defect. . 2023; 195 - 202. 10.5505/ejm.2023.24356
Vancouver	AKYOL M,Denizler Ebiri F,Taspinar F,yüksek v,Tuncer O,Arslan M,taspinar m The Role of Matrix Metalloproteinase As Biomarkers For Neural Tube Defect. . 2023; 195 - 202. 10.5505/ejm.2023.24356
IEEE	AKYOL M,Denizler Ebiri F,Taspinar F,yüksek v,Tuncer O,Arslan M,taspinar m "The Role of Matrix Metalloproteinase As Biomarkers For Neural Tube Defect." , ss.195 - 202, 2023. 10.5505/ejm.2023.24356
ISNAD	AKYOL, MEHMET EDIP vd. "The Role of Matrix Metalloproteinase As Biomarkers For Neural Tube Defect". (2023), 195-202. https://doi.org/10.5505/ejm.2023.24356

APA	AKYOL M, Denizler Ebiri F, Taspinar F, yüksek v, Tuncer O, Arslan M, taspinar m (2023). The Role of Matrix Metalloproteinase As Biomarkers For Neural Tube Defect. Eastern Journal of Medicine, 28(1), 195 - 202. 10.5505/ejm.2023.24356
Chicago	AKYOL MEHMET EDIP,Denizler Ebiri Farika Nur,Taspinar Filiz,yüksek veysel,Tuncer Oğuz,Arslan Mehmet,taspinar mehmet The Role of Matrix Metalloproteinase As Biomarkers For Neural Tube Defect. Eastern Journal of Medicine 28, no.1 (2023): 195 - 202. 10.5505/ejm.2023.24356
MLA	AKYOL MEHMET EDIP,Denizler Ebiri Farika Nur,Taspinar Filiz,yüksek veysel,Tuncer Oğuz,Arslan Mehmet,taspinar mehmet The Role of Matrix Metalloproteinase As Biomarkers For Neural Tube Defect. Eastern Journal of Medicine, vol.28, no.1, 2023, ss.195 - 202. 10.5505/ejm.2023.24356
AMA	AKYOL M,Denizler Ebiri F,Taspinar F,yüksek v,Tuncer O,Arslan M,taspinar m The Role of Matrix Metalloproteinase As Biomarkers For Neural Tube Defect. Eastern Journal of Medicine. 2023; 28(1): 195 - 202. 10.5505/ejm.2023.24356
Vancouver	AKYOL M,Denizler Ebiri F,Taspinar F,yüksek v,Tuncer O,Arslan M,taspinar m The Role of Matrix Metalloproteinase As Biomarkers For Neural Tube Defect. Eastern Journal of Medicine. 2023; 28(1): 195 - 202. 10.5505/ejm.2023.24356
IEEE	AKYOL M,Denizler Ebiri F,Taspinar F,yüksek v,Tuncer O,Arslan M,taspinar m "The Role of Matrix Metalloproteinase As Biomarkers For Neural Tube Defect." Eastern Journal of Medicine, 28, ss.195 - 202, 2023. 10.5505/ejm.2023.24356
ISNAD	AKYOL, MEHMET EDIP vd. "The Role of Matrix Metalloproteinase As Biomarkers For Neural Tube Defect". Eastern Journal of Medicine 28/1 (2023), 195-202. https://doi.org/10.5505/ejm.2023.24356