Wound healing effects of bee venom on adipose tissue derived mesenchymal stem cells
Yıl: 2023 Cilt: 94 Sayı: 1 Sayfa Aralığı: 59 - 66 Metin Dili: İngilizce DOI: 10.33188/vetheder.1183380 İndeks Tarihi: 26-08-2023
Wound healing effects of bee venom on adipose tissue derived mesenchymal stem cells
Öz: The objective of this study is to determine the effects of bee venom on the proliferation capacity of mesenchymal stem cells and wound healing. For this purpose, mesenchymal stem cells were isolated from canine adipose tissue and bee venom samples were collected from Apis mellifera anatoliaca in Muğla province of Türkiye. Cell viability test was performed on mesenchymal stem cells exposed to various concentrations (40 ppm, 20 ppm, 10 ppm, 5 ppm, 2.5 ppm, 1.25 ppm, 0.625 ppm and 0.312 ppm) of bee venom. And wound healing test was performed on cells treated with the doses (5 ppm, 2.5 ppm, 1.25 ppm, 0.625 ppm) and imaged every two hours for 16 hours. According to the results of our study's cell proliferation assay and wound healing test, bee venom had no proliferative effect on mesenchymal stem cells within the defined dose range. The study's outcomes may be enhanced by investigating the effect of bee venom on mesenchymal stem cells in combination with other substances or by improving the bee venom's purification process. Even while we have a better understanding of the mechanisms of action of bee venom components, there are still a lot of unanswered questions on the subject. It is believed that figuring out how bee venom affects wound healing may be useful for advancing wound care in both veterinary and human medicine.
Anahtar Kelime: Arı zehirinin yağ dokusu kaynaklı mezenkimal kök hücre üzerindeki yara iyileştirici etkileri
Öz: Bu çalışmanın amacı, arı zehirinin mezenkimal kök hücrelerin çoğalma kapasitesi ve yara iyileşmesi üzerindeki etkilerini belirlemektir. Bu amaçla köpek yağ dokusundan mezenkimal kök hücreler izole edilmiş ve Muğla çevresinde bulunan Apis mellifera anatoliaca'dan arı zehiri örnekleri toplanmıştır. Farklı konsantrasyonlarda arı zehirine maruz bırakılan mezenkimal kök hücreler üzerinde hücre canlılığı testi (40 ppm, 20 ppm, 10 ppm, 5 ppm, 2.5 ppm, 1.25 ppm, 0,625 ppm ve 0.312 ppm) yapılmıştır. Hücreler üzerinde 5 ppm, 2.5 ppm, 1.25 ppm, 0.625 ppm arı zehri kullanılarak yara iyileştirme deneyi yapılmış ve 16 saat boyunca her iki saatte bir görüntülenmiştir. Çalışmanın hücre canlılığı ve yara iyileşme deneylerinin sonuçlarına göre, arı zehirinin köpek yağ dokusu kaynaklı mezenkimal kök hücreler üzerinde tanımlanan doz aralığında proliferatif bir etkisi olmamıştır. Çalışmanın sonuçları, diğer maddelerle birlikte mezenkimal kök hücreler üzerindeki arı zehirinin etkisini araştırarak veya arı zehirinin saflaştırma sürecini geliştirerek iyileştirilebilir. Arı zehiri bileşenlerinin etki mekanizmalarını daha iyi anlamış olsak da konuyla ilgili hala cevaplanmamış birçok soru bulunmaktadır. Arı zehrinin yara iyileşmesi üzerindeki etkisinin belirlenmesinin hem veteriner hekimlikte hem de beşeri hekimlikte yara tedavisini kolaylaştırmada avantaj sağlayacağı düşünülmektedir.
Anahtar Kelime: Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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| APA | ÖZGENÇ Ö, Sevin S (2023). Wound healing effects of bee venom on adipose tissue derived mesenchymal stem cells. , 59 - 66. 10.33188/vetheder.1183380 |
| Chicago | ÖZGENÇ Özge,Sevin Sedat Wound healing effects of bee venom on adipose tissue derived mesenchymal stem cells. (2023): 59 - 66. 10.33188/vetheder.1183380 |
| MLA | ÖZGENÇ Özge,Sevin Sedat Wound healing effects of bee venom on adipose tissue derived mesenchymal stem cells. , 2023, ss.59 - 66. 10.33188/vetheder.1183380 |
| AMA | ÖZGENÇ Ö,Sevin S Wound healing effects of bee venom on adipose tissue derived mesenchymal stem cells. . 2023; 59 - 66. 10.33188/vetheder.1183380 |
| Vancouver | ÖZGENÇ Ö,Sevin S Wound healing effects of bee venom on adipose tissue derived mesenchymal stem cells. . 2023; 59 - 66. 10.33188/vetheder.1183380 |
| IEEE | ÖZGENÇ Ö,Sevin S "Wound healing effects of bee venom on adipose tissue derived mesenchymal stem cells." , ss.59 - 66, 2023. 10.33188/vetheder.1183380 |
| ISNAD | ÖZGENÇ, Özge - Sevin, Sedat. "Wound healing effects of bee venom on adipose tissue derived mesenchymal stem cells". (2023), 59-66. https://doi.org/10.33188/vetheder.1183380 |
| APA | ÖZGENÇ Ö, Sevin S (2023). Wound healing effects of bee venom on adipose tissue derived mesenchymal stem cells. Veteriner Hekimler Derneği Dergisi, 94(1), 59 - 66. 10.33188/vetheder.1183380 |
| Chicago | ÖZGENÇ Özge,Sevin Sedat Wound healing effects of bee venom on adipose tissue derived mesenchymal stem cells. Veteriner Hekimler Derneği Dergisi 94, no.1 (2023): 59 - 66. 10.33188/vetheder.1183380 |
| MLA | ÖZGENÇ Özge,Sevin Sedat Wound healing effects of bee venom on adipose tissue derived mesenchymal stem cells. Veteriner Hekimler Derneği Dergisi, vol.94, no.1, 2023, ss.59 - 66. 10.33188/vetheder.1183380 |
| AMA | ÖZGENÇ Ö,Sevin S Wound healing effects of bee venom on adipose tissue derived mesenchymal stem cells. Veteriner Hekimler Derneği Dergisi. 2023; 94(1): 59 - 66. 10.33188/vetheder.1183380 |
| Vancouver | ÖZGENÇ Ö,Sevin S Wound healing effects of bee venom on adipose tissue derived mesenchymal stem cells. Veteriner Hekimler Derneği Dergisi. 2023; 94(1): 59 - 66. 10.33188/vetheder.1183380 |
| IEEE | ÖZGENÇ Ö,Sevin S "Wound healing effects of bee venom on adipose tissue derived mesenchymal stem cells." Veteriner Hekimler Derneği Dergisi, 94, ss.59 - 66, 2023. 10.33188/vetheder.1183380 |
| ISNAD | ÖZGENÇ, Özge - Sevin, Sedat. "Wound healing effects of bee venom on adipose tissue derived mesenchymal stem cells". Veteriner Hekimler Derneği Dergisi 94/1 (2023), 59-66. https://doi.org/10.33188/vetheder.1183380 |
