Bluetongue virus (BTV) is transmitted by vectors that cause significant losses in Europe and has a teratogenic effect, especially in sheep. This study was carried out to investigate the seroprevalence of MDV in Samsun, Sinop, Ordu, Giresun, and Amasya provinces of Turkey's Central Black Sea region. Serum samples were collected from 400 sheep, 80 from each province. Ab ELISA test technique was used to determine the presence of antibodies specific to BTV. As a result of this test, ten sheep (2.5%) were determined to be positive for BTV antibodies. While 2.5% and 10% positivity rates were detected in Amasya and Sinop provinces, no positive samples were found in Samsun, Ordu, and Giresun provinces. The obtained data showed that bluetongue infection had a low seroprevalence in the region of this study. Seropositivity detection of BTV in the Central Black Sea region, where vaccination against the causative agent is not performed, shows the necessity of vaccination studies.