Carrot (Daucus carota), which can be orange, yellow, purple, white and red in color due to the difference in the pigments it contains, is a very rich food in terms of carotene and ascorbic acid. In this study, the effects of exogenous Glutamine (Gln) pre-treatments ( 1, 2, 3, 4 mM) on the some germination parameters of carrot seeds (orange, yellow, purple), which are known to be sensitive to salt stress according to the cultivar, were investigated under salt stress conditions (150 mM NaCl). For this purpose, germination percentage (GP), mean germination time (MGT), germination rate coefficient (CVG), germination rate index (GRI), germination index (GI), which are some of the important germination parameters, were determined. According to the results of analysis of variance, it was determined that there was an interaction between cultivars and applications and all germination parameters under salt stress conditions (***p<0.001). The effect of Gln pre-treatments on germination under salt stress and unstressed conditions differed according to the application dose and cultivar. Glutamine application under salt stress had no effect on germination except for the orange carrot cv. In this cultivar, 1 mM pre-treatment had a positive effect on germination parameters under salt stress conditions. In unstressed conditions, although 3 mM Gln pretreatment in orange carrot cv. and 4 mM Gln in yellow carrot cv. had a positive effect on germination parameters compared to control, the highest germination in purple carrot cv. was obtained in control seeds.