Clinical Validation and Evaluation of a Colorimetric SARS-CoV-2 RT-LAMP Assay Against RT-PCR
Yıl: 2023 Cilt: 5 Sayı: 2 Sayfa Aralığı: 136 - 143 Metin Dili: İngilizce DOI: 10.36519/idcm.2023.210 İndeks Tarihi: 05-07-2023
Clinical Validation and Evaluation of a Colorimetric SARS-CoV-2 RT-LAMP Assay Against RT-PCR
Öz: Objective: Reverse transcriptase loop-mediated isothermal amplification (RT-LAMP) is one of the time-saving, accurate, and cost-effective alternative methods to real-time polymer- ase chain reaction (RT-PCR). This study aimed to identify the robustness of a colorimetric RT-LAMP assay kit that we developed, detecting SARS-COV-2 viral RNA within 30 minutes using a primer set special to the N gene against RT-PCR, the gold standard. Materials and Methods: Both symptomatic and asymptomatic subjects were included from a single university hospital and the status of both RT-PCR and RT-LAMP assay results were compared, and the consistency of these two assays was analyzed. Results: We showed that the RT-LAMP and RT-PCR assay results confirmed 90% consistency. When we consider the epidemiologic, clinical, and radiologic evaluation, the consistency reached 97%. Conclusion: The results revealed that the colorimetric RT-LAMP assay was efficient, robust, and rapid to be used as in vitro diagnostic tool to display competitiveness compared with RT-PCR.
Anahtar Kelime: Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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| APA | Erdem M, Andac A, Özketen A, Karahan G, Tozluyurt A, Palaz F, ALP A, Unal S (2023). Clinical Validation and Evaluation of a Colorimetric SARS-CoV-2 RT-LAMP Assay Against RT-PCR. , 136 - 143. 10.36519/idcm.2023.210 |
| Chicago | Erdem Murat,Andac Ayse,Özketen Ahmet Çağlar,Karahan Gizem,Tozluyurt Abdullah,Palaz Fahreddin,ALP ALPASLAN,Unal Serhat Clinical Validation and Evaluation of a Colorimetric SARS-CoV-2 RT-LAMP Assay Against RT-PCR. (2023): 136 - 143. 10.36519/idcm.2023.210 |
| MLA | Erdem Murat,Andac Ayse,Özketen Ahmet Çağlar,Karahan Gizem,Tozluyurt Abdullah,Palaz Fahreddin,ALP ALPASLAN,Unal Serhat Clinical Validation and Evaluation of a Colorimetric SARS-CoV-2 RT-LAMP Assay Against RT-PCR. , 2023, ss.136 - 143. 10.36519/idcm.2023.210 |
| AMA | Erdem M,Andac A,Özketen A,Karahan G,Tozluyurt A,Palaz F,ALP A,Unal S Clinical Validation and Evaluation of a Colorimetric SARS-CoV-2 RT-LAMP Assay Against RT-PCR. . 2023; 136 - 143. 10.36519/idcm.2023.210 |
| Vancouver | Erdem M,Andac A,Özketen A,Karahan G,Tozluyurt A,Palaz F,ALP A,Unal S Clinical Validation and Evaluation of a Colorimetric SARS-CoV-2 RT-LAMP Assay Against RT-PCR. . 2023; 136 - 143. 10.36519/idcm.2023.210 |
| IEEE | Erdem M,Andac A,Özketen A,Karahan G,Tozluyurt A,Palaz F,ALP A,Unal S "Clinical Validation and Evaluation of a Colorimetric SARS-CoV-2 RT-LAMP Assay Against RT-PCR." , ss.136 - 143, 2023. 10.36519/idcm.2023.210 |
| ISNAD | Erdem, Murat vd. "Clinical Validation and Evaluation of a Colorimetric SARS-CoV-2 RT-LAMP Assay Against RT-PCR". (2023), 136-143. https://doi.org/10.36519/idcm.2023.210 |
| APA | Erdem M, Andac A, Özketen A, Karahan G, Tozluyurt A, Palaz F, ALP A, Unal S (2023). Clinical Validation and Evaluation of a Colorimetric SARS-CoV-2 RT-LAMP Assay Against RT-PCR. Infectious diseases and clinical microbiology (Online), 5(2), 136 - 143. 10.36519/idcm.2023.210 |
| Chicago | Erdem Murat,Andac Ayse,Özketen Ahmet Çağlar,Karahan Gizem,Tozluyurt Abdullah,Palaz Fahreddin,ALP ALPASLAN,Unal Serhat Clinical Validation and Evaluation of a Colorimetric SARS-CoV-2 RT-LAMP Assay Against RT-PCR. Infectious diseases and clinical microbiology (Online) 5, no.2 (2023): 136 - 143. 10.36519/idcm.2023.210 |
| MLA | Erdem Murat,Andac Ayse,Özketen Ahmet Çağlar,Karahan Gizem,Tozluyurt Abdullah,Palaz Fahreddin,ALP ALPASLAN,Unal Serhat Clinical Validation and Evaluation of a Colorimetric SARS-CoV-2 RT-LAMP Assay Against RT-PCR. Infectious diseases and clinical microbiology (Online), vol.5, no.2, 2023, ss.136 - 143. 10.36519/idcm.2023.210 |
| AMA | Erdem M,Andac A,Özketen A,Karahan G,Tozluyurt A,Palaz F,ALP A,Unal S Clinical Validation and Evaluation of a Colorimetric SARS-CoV-2 RT-LAMP Assay Against RT-PCR. Infectious diseases and clinical microbiology (Online). 2023; 5(2): 136 - 143. 10.36519/idcm.2023.210 |
| Vancouver | Erdem M,Andac A,Özketen A,Karahan G,Tozluyurt A,Palaz F,ALP A,Unal S Clinical Validation and Evaluation of a Colorimetric SARS-CoV-2 RT-LAMP Assay Against RT-PCR. Infectious diseases and clinical microbiology (Online). 2023; 5(2): 136 - 143. 10.36519/idcm.2023.210 |
| IEEE | Erdem M,Andac A,Özketen A,Karahan G,Tozluyurt A,Palaz F,ALP A,Unal S "Clinical Validation and Evaluation of a Colorimetric SARS-CoV-2 RT-LAMP Assay Against RT-PCR." Infectious diseases and clinical microbiology (Online), 5, ss.136 - 143, 2023. 10.36519/idcm.2023.210 |
| ISNAD | Erdem, Murat vd. "Clinical Validation and Evaluation of a Colorimetric SARS-CoV-2 RT-LAMP Assay Against RT-PCR". Infectious diseases and clinical microbiology (Online) 5/2 (2023), 136-143. https://doi.org/10.36519/idcm.2023.210 |
