TY  - JOUR
TI  - Clinical Validation and Evaluation of a Colorimetric SARS-CoV-2 RT-LAMP Assay Against RT-PCR
AB  - Objective: Reverse transcriptase loop-mediated isothermal amplification (RT-LAMP) is one of the time-saving, accurate, and cost-effective alternative methods to real-time polymer- ase chain reaction (RT-PCR). This study aimed to identify the robustness of a colorimetric RT-LAMP assay kit that we developed, detecting SARS-COV-2 viral RNA within 30 minutes using a primer set special to the N gene against RT-PCR, the gold standard. Materials and Methods: Both symptomatic and asymptomatic subjects were included from a single university hospital and the status of both RT-PCR and RT-LAMP assay results were compared, and the consistency of these two assays was analyzed. Results: We showed that the RT-LAMP and RT-PCR assay results confirmed 90% consistency. When we consider the epidemiologic, clinical, and radiologic evaluation, the consistency reached 97%. Conclusion: The results revealed that the colorimetric RT-LAMP assay was efficient, robust, and rapid to be used as in vitro diagnostic tool to display competitiveness compared with RT-PCR.
AU  - Unal, Serhat
AU  - ALP, ALPASLAN
AU  - Andac, Ayse
AU  - Erdem, Murat
AU  - Özketen, Ahmet Çağlar
AU  - Karahan, Gizem
AU  - Tozluyurt, Abdullah
AU  - Palaz, Fahreddin
DO  - 10.36519/idcm.2023.210
PY  - 2023
JO  - Infectious diseases and clinical microbiology (Online)
VL  - 5
IS  - 2
SN  - 2667-646X
SP  - 136
EP  - 143
DB  - TRDizin
UR  - http://search/yayin/detay/1183921
ER  -