Role of SATB2 5' Untranslated Region Promoter Methylation in Formation of Non-syndromic Cleft Palate Only

zheng, Yingling; Shu, Shenyou; Ji, Yingwen; yandan, xie; zhang, ming; Zhou, Jieyan; Peng, Li-hong

doi:10.14744/ejmo.2023.41377

Role of SATB2 5' Untranslated Region Promoter Methylation in Formation of Non-syndromic Cleft Palate Only

Yingwen Ji, (The Cleft Lip and Palate Treatment Center, Second Affiliated Hospital of Shantou University Medical College, Shantou, Guangdong China)

xie yandan, (Department of Cardiology, First Affiliated Hospital of Shantou University Medical College, Shantou, Guangdong, China)

Ming ZHANG, (The Cleft Lip and Palate Treatment Center, Second Affiliated Hospital of Shantou University Medical College, Shantou, Guangdong China)

Jieyan ZHOU, (The Cleft Lip and Palate Treatment Center, Second Affiliated Hospital of Shantou University Medical College, Shantou, Guangdong China)

Li-hong Peng, (The Cleft Lip and Palate Treatment Center, Second Affiliated Hospital of Shantou University Medical College, Shantou, Guangdong China)

Yingling ZHENG, (The Cleft Lip and Palate Treatment Center, Second Affiliated Hospital of Shantou University Medical College, Shantou, Guangdong China)

Shenyou SHU (The Cleft Lip and Palate Treatment Center, Second Affiliated Hospital of Shantou University Medical College, Shantou, Guangdong China)

Eurasian Journal of Medicine and Oncology

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Yıl: 2023 Cilt: 7 Sayı: 2 Sayfa Aralığı: 165 - 173 Metin Dili: İngilizce DOI: 10.14744/ejmo.2023.41377 İndeks Tarihi: 05-07-2023

Role of SATB2 5' Untranslated Region Promoter Methylation in Formation of Non-syndromic Cleft Palate Only

Öz:

Objectives: Accumulating evidence has implicated DNA methylation in the development of non-syndromic cleft pal- ate only (NSCPO); however, little is known about the underlying epigenetic mechanism. This study was to elucidate the role of SATB2 5'untranslated region (UTR) promoter methylation in formation of NSCPO. Methods: DNA methylation profiling was performed on discarded human palatal tissue after repair of NSCPO (case) or maxillofacial and palate trauma (control), using an Illumina 850K-EPIC BeadChip methylation array. The SATB2 5'UTR promoter methylation level was confirmed by pyrosequencing. Results: Five CpG sites (cg14273610, cg22334352, cg25103650, cg22845542 and cg06199336) in the SATB2 5'UTR pro- moter was hypermethylated in cases compared with controls (P<0.05). Pyrosequencing revealed a mean methylation rate of 31.81% vs. 16.45% (p=0.0019) at the cg14273610 CpG site, 22.12% vs. 9.28% (p=0.0102) at the cg22334352 CpG site, 24.41% vs. 8.74% (p=0.0003) at the cg25103650 CpG site, 51.66% vs. 23.97%(p=0.0165) at the cg22845542 CpG site and 31.05% vs. 16.43% (p=0.0091) at the cg06199336 CpG site for cases and controls, respectively. The pyrosequencing results were consistent with those from the Illumina 850K-EPIC methylation BeadChip array. Conclusion: Our results suggested that the SATB2 may be responsible for NSCPO formation and could be a potential biomarker for NSCPO.

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Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	Ji Y, yandan x, zhang m, Zhou J, Peng L, zheng Y, Shu S (2023). Role of SATB2 5' Untranslated Region Promoter Methylation in Formation of Non-syndromic Cleft Palate Only. , 165 - 173. 10.14744/ejmo.2023.41377
Chicago	Ji Yingwen,yandan xie,zhang ming,Zhou Jieyan,Peng Li-hong,zheng Yingling,Shu Shenyou Role of SATB2 5' Untranslated Region Promoter Methylation in Formation of Non-syndromic Cleft Palate Only. (2023): 165 - 173. 10.14744/ejmo.2023.41377
MLA	Ji Yingwen,yandan xie,zhang ming,Zhou Jieyan,Peng Li-hong,zheng Yingling,Shu Shenyou Role of SATB2 5' Untranslated Region Promoter Methylation in Formation of Non-syndromic Cleft Palate Only. , 2023, ss.165 - 173. 10.14744/ejmo.2023.41377
AMA	Ji Y,yandan x,zhang m,Zhou J,Peng L,zheng Y,Shu S Role of SATB2 5' Untranslated Region Promoter Methylation in Formation of Non-syndromic Cleft Palate Only. . 2023; 165 - 173. 10.14744/ejmo.2023.41377
Vancouver	Ji Y,yandan x,zhang m,Zhou J,Peng L,zheng Y,Shu S Role of SATB2 5' Untranslated Region Promoter Methylation in Formation of Non-syndromic Cleft Palate Only. . 2023; 165 - 173. 10.14744/ejmo.2023.41377
IEEE	Ji Y,yandan x,zhang m,Zhou J,Peng L,zheng Y,Shu S "Role of SATB2 5' Untranslated Region Promoter Methylation in Formation of Non-syndromic Cleft Palate Only." , ss.165 - 173, 2023. 10.14744/ejmo.2023.41377
ISNAD	Ji, Yingwen vd. "Role of SATB2 5' Untranslated Region Promoter Methylation in Formation of Non-syndromic Cleft Palate Only". (2023), 165-173. https://doi.org/10.14744/ejmo.2023.41377

APA	Ji Y, yandan x, zhang m, Zhou J, Peng L, zheng Y, Shu S (2023). Role of SATB2 5' Untranslated Region Promoter Methylation in Formation of Non-syndromic Cleft Palate Only. Eurasian Journal of Medicine and Oncology, 7(2), 165 - 173. 10.14744/ejmo.2023.41377
Chicago	Ji Yingwen,yandan xie,zhang ming,Zhou Jieyan,Peng Li-hong,zheng Yingling,Shu Shenyou Role of SATB2 5' Untranslated Region Promoter Methylation in Formation of Non-syndromic Cleft Palate Only. Eurasian Journal of Medicine and Oncology 7, no.2 (2023): 165 - 173. 10.14744/ejmo.2023.41377
MLA	Ji Yingwen,yandan xie,zhang ming,Zhou Jieyan,Peng Li-hong,zheng Yingling,Shu Shenyou Role of SATB2 5' Untranslated Region Promoter Methylation in Formation of Non-syndromic Cleft Palate Only. Eurasian Journal of Medicine and Oncology, vol.7, no.2, 2023, ss.165 - 173. 10.14744/ejmo.2023.41377
AMA	Ji Y,yandan x,zhang m,Zhou J,Peng L,zheng Y,Shu S Role of SATB2 5' Untranslated Region Promoter Methylation in Formation of Non-syndromic Cleft Palate Only. Eurasian Journal of Medicine and Oncology. 2023; 7(2): 165 - 173. 10.14744/ejmo.2023.41377
Vancouver	Ji Y,yandan x,zhang m,Zhou J,Peng L,zheng Y,Shu S Role of SATB2 5' Untranslated Region Promoter Methylation in Formation of Non-syndromic Cleft Palate Only. Eurasian Journal of Medicine and Oncology. 2023; 7(2): 165 - 173. 10.14744/ejmo.2023.41377
IEEE	Ji Y,yandan x,zhang m,Zhou J,Peng L,zheng Y,Shu S "Role of SATB2 5' Untranslated Region Promoter Methylation in Formation of Non-syndromic Cleft Palate Only." Eurasian Journal of Medicine and Oncology, 7, ss.165 - 173, 2023. 10.14744/ejmo.2023.41377
ISNAD	Ji, Yingwen vd. "Role of SATB2 5' Untranslated Region Promoter Methylation in Formation of Non-syndromic Cleft Palate Only". Eurasian Journal of Medicine and Oncology 7/2 (2023), 165-173. https://doi.org/10.14744/ejmo.2023.41377