Whole-Genome Sequencing-Based Characterization of Listeria monocytogenes from Food and Animal Clinical Cases
Yıl: 2023 Cilt: 29 Sayı: 3 Sayfa Aralığı: 221 - 230 Metin Dili: İngilizce DOI: 10.9775/kvfd.2023.28970 İndeks Tarihi: 10-07-2023
Whole-Genome Sequencing-Based Characterization of Listeria monocytogenes from Food and Animal Clinical Cases
Öz: Listeriosis is a rare but severe foodborne infection caused by Listeria monocytogenes. In this study, we performed comparative whole-genome sequencing (WGS) on 28 Listeria monocytogenes from seven invasive listeriosis cases in animals and 21 food samples in Türkiye for the first time. Food isolates were delineated into eleven clonal complexes (CCs), namely CC1, CC2, CC3, CC8, CC9, CC20, CC69, CC124, CC155, CC204, ST3002. The isolates from meningoencephalitis cases were associated with CC1, whereas CC9 and CC7 were associated with the isolates from sheep abortus cases. All the isolates carried the fosX, lin, norB, and sul genes. In addition, emrC (n=15), bcrC (n=4), emrE (n=2), qacA (n=1), cadA (n=5) and cadC (n=1) genes, conferring resistance to stress and disinfectants were detected. Listeria pathogenicity island (LIPI)-1 and LIPI-2 were distributed in all isolates, but LIPI-3 was closely related to CC1, CC3, and ST3002 isolates. LIPI-4 was not found in any of the L. monocytogenes isolates. The Inc18(rep25) and Inc18(rep26) plasmids were found in 16 (57.1%) isolates. A total of 15 different intact prophage genomes ranging from one to three were detected in the genomes of 24 isolates. The hypervirulent CC1 and CC2 clones that pose a significant threat to food safety and public health were detected among food isolates. These findings highlight the importance of continuous surveillance of hypervirulent L. monocytogenes strains in different settings.
Anahtar Kelime: Gıda ve Hayvan Klinik Listeria monocytogenes İzolatlarının Tam Genom Dizilimine Dayalı Karakterizasyonu
Öz: Listeriosis, Listeria monocytogenes’in neden olduğu nadir görülen fakat ciddi klinik seyre sahip gıda kaynaklı bir enfeksiyondur. Bu çalışmada, hayvanlardaki invaziv listeriozis vakalarından (n=7) ve farklı gıda örneklerinden (n=21) izole edilen 28 Listeria monocytogenes suşunun karşılaştırmalı tam genom dizileme (WGS) ile analizi yapıldı. Gıda izolatları CC1, CC2, CC3, CC8, CC9, CC20, CC69, CC124, CC155, CC204, ST3002 olmak üzere onbir klonal komplekse (CC) ayrıldı. Meningoensefalit vakalarına ait izolatlar CC1’e ait iken, koyun abortus vakalarına ait izolatlar CC9 ve CC7’e ait bulundu. Tüm izolatlarda fosX, lin, norB ve sul genleri belirlendi. Ayrıca, değişen oranlarda stres ve dezenfektan direncine aracılık eden emrC (n=15), bcrC (n=4), emrE (n=2), qacA (n=1), cadA (n=5) ve cadC (n=1) genleri tespit edildi. Listeria patojenite adası (LIPI)-1 ve LIPI-2 tüm izolatlarda tespit edilirken; LIPI-3 CC1, CC3 ve ST3002’e ait izolatlar ile yakın ilişkili bulundu. LIPI-4 L. monocytogenes izolatlarının hiçbirinde bulunmadı. Inc18(rep25) ve Inc18(rep26) plazmidleri 16 (%57.1) izolatta bulundu. Yirmidört izolatta bir - üç arasında değişen toplam 15 farklı intakt profaj genomu tespit edildi. Bu çalışmada gıda izolatları arasında gıda güvenliği ve halk sağlığı için önemli bir tehdit oluşturan hipervirülent CC1 ve CC2 klonları tespit edilmiştir. Bu bulgular, farklı ortamlarda hipervirülent L. monocytogenes suşlarının sürekli izlenmesinin önemini vurgulamaktadır.
Anahtar Kelime: Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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| APA | ASLANTAŞ Ö, Buyukaltay K, KESKIN O, GÜLLÜ YÜCETEPE A, ADIGÜZEL A (2023). Whole-Genome Sequencing-Based Characterization of Listeria monocytogenes from Food and Animal Clinical Cases. , 221 - 230. 10.9775/kvfd.2023.28970 |
| Chicago | ASLANTAŞ Özkan,Buyukaltay Kaan,KESKIN Oktay,GÜLLÜ YÜCETEPE AYFER,ADIGÜZEL ADEM Whole-Genome Sequencing-Based Characterization of Listeria monocytogenes from Food and Animal Clinical Cases. (2023): 221 - 230. 10.9775/kvfd.2023.28970 |
| MLA | ASLANTAŞ Özkan,Buyukaltay Kaan,KESKIN Oktay,GÜLLÜ YÜCETEPE AYFER,ADIGÜZEL ADEM Whole-Genome Sequencing-Based Characterization of Listeria monocytogenes from Food and Animal Clinical Cases. , 2023, ss.221 - 230. 10.9775/kvfd.2023.28970 |
| AMA | ASLANTAŞ Ö,Buyukaltay K,KESKIN O,GÜLLÜ YÜCETEPE A,ADIGÜZEL A Whole-Genome Sequencing-Based Characterization of Listeria monocytogenes from Food and Animal Clinical Cases. . 2023; 221 - 230. 10.9775/kvfd.2023.28970 |
| Vancouver | ASLANTAŞ Ö,Buyukaltay K,KESKIN O,GÜLLÜ YÜCETEPE A,ADIGÜZEL A Whole-Genome Sequencing-Based Characterization of Listeria monocytogenes from Food and Animal Clinical Cases. . 2023; 221 - 230. 10.9775/kvfd.2023.28970 |
| IEEE | ASLANTAŞ Ö,Buyukaltay K,KESKIN O,GÜLLÜ YÜCETEPE A,ADIGÜZEL A "Whole-Genome Sequencing-Based Characterization of Listeria monocytogenes from Food and Animal Clinical Cases." , ss.221 - 230, 2023. 10.9775/kvfd.2023.28970 |
| ISNAD | ASLANTAŞ, Özkan vd. "Whole-Genome Sequencing-Based Characterization of Listeria monocytogenes from Food and Animal Clinical Cases". (2023), 221-230. https://doi.org/10.9775/kvfd.2023.28970 |
| APA | ASLANTAŞ Ö, Buyukaltay K, KESKIN O, GÜLLÜ YÜCETEPE A, ADIGÜZEL A (2023). Whole-Genome Sequencing-Based Characterization of Listeria monocytogenes from Food and Animal Clinical Cases. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 29(3), 221 - 230. 10.9775/kvfd.2023.28970 |
| Chicago | ASLANTAŞ Özkan,Buyukaltay Kaan,KESKIN Oktay,GÜLLÜ YÜCETEPE AYFER,ADIGÜZEL ADEM Whole-Genome Sequencing-Based Characterization of Listeria monocytogenes from Food and Animal Clinical Cases. Kafkas Üniversitesi Veteriner Fakültesi Dergisi 29, no.3 (2023): 221 - 230. 10.9775/kvfd.2023.28970 |
| MLA | ASLANTAŞ Özkan,Buyukaltay Kaan,KESKIN Oktay,GÜLLÜ YÜCETEPE AYFER,ADIGÜZEL ADEM Whole-Genome Sequencing-Based Characterization of Listeria monocytogenes from Food and Animal Clinical Cases. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, vol.29, no.3, 2023, ss.221 - 230. 10.9775/kvfd.2023.28970 |
| AMA | ASLANTAŞ Ö,Buyukaltay K,KESKIN O,GÜLLÜ YÜCETEPE A,ADIGÜZEL A Whole-Genome Sequencing-Based Characterization of Listeria monocytogenes from Food and Animal Clinical Cases. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 2023; 29(3): 221 - 230. 10.9775/kvfd.2023.28970 |
| Vancouver | ASLANTAŞ Ö,Buyukaltay K,KESKIN O,GÜLLÜ YÜCETEPE A,ADIGÜZEL A Whole-Genome Sequencing-Based Characterization of Listeria monocytogenes from Food and Animal Clinical Cases. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 2023; 29(3): 221 - 230. 10.9775/kvfd.2023.28970 |
| IEEE | ASLANTAŞ Ö,Buyukaltay K,KESKIN O,GÜLLÜ YÜCETEPE A,ADIGÜZEL A "Whole-Genome Sequencing-Based Characterization of Listeria monocytogenes from Food and Animal Clinical Cases." Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 29, ss.221 - 230, 2023. 10.9775/kvfd.2023.28970 |
| ISNAD | ASLANTAŞ, Özkan vd. "Whole-Genome Sequencing-Based Characterization of Listeria monocytogenes from Food and Animal Clinical Cases". Kafkas Üniversitesi Veteriner Fakültesi Dergisi 29/3 (2023), 221-230. https://doi.org/10.9775/kvfd.2023.28970 |
