Evaluation of Antimicrobial and Antibiofilm Efficacy of Bee Venom and Exosome Against Escherichia coli K99 Strain
Yıl: 2023 Cilt: 29 Sayı: 3 Sayfa Aralığı: 239 - 246 Metin Dili: İngilizce DOI: 10.9775/kvfd.2023.29132 İndeks Tarihi: 10-07-2023
Evaluation of Antimicrobial and Antibiofilm Efficacy of Bee Venom and Exosome Against Escherichia coli K99 Strain
Öz: Escherichia coli K99 (F5) strain is one of the bacterial agents that cause calf deaths. F5 is an adhesin that allows pathogenic E. coli attach to the small intestine cells and colonize there. The presence of F5 in E. coli strains in isolated bacteria is classified as Enterotoxigenic. Bee venom and bee venom-derived exosomes are bioactive compounds that exhibit antimicrobial and antibiofilm activity. The aim of this study is to demonstrate the antimicrobial and antibiofilm activity of bee venom and bee venom-derived exosomes against E. coli, which cause calf diarrhea. Bee venom-derived exosomes and bee venom effects against E. coli strains were determined by using Minimal inhibition concentration (MIC), antibiofilm activity, fractional inhibition concentrations (FIC), and measurement of L929 cells viability ratio. Cell damage was examined under a fluorescent microscope by an immunohistochemical method. In our study, the MIC value of the bee venom-derived exosome was determined as 1.95 μg/mL. A synergistic effect was detected with a value of 0.44 in combinations of amoxicillin with clavulanic acid. Antibiofilm activity was determined at the rate of 48.8% in bee venom, while bee venom-derived exosomes inhibited the biofilm layer by 60.4%. In L929 cell lines, combination groups have been reported to reduce viability. Bee venom-derived exosomes are more effective on bacteria than pure bee venom. In conclusion; It is important that the bee venom-derived exosome, which is a biocompatible molecule and acts as a cargo element, exhibits antimicrobial and especially antibiofilm activity and is an alternative approach against increasing antibiotic resistance.
Anahtar Kelime: Escherichia coli K99 Suşuna Karşı Arı Zehiri ve Arı Zehrinden İzole Edilen Eksozomun Antimikrobiyal ve Antibiyofilm Etkinliğinin Değerlendirilmesi
Öz: Escherichia coli K99 (F5) suşu buzağı ölümlerine sebep olan bakteriyal etkenlerden biridir. F5, patojenik E. coli suşlarının bağırsak hücrelerine yapışmasını ve ince bağırsağı kolonize etmesini sağlayan bir adezindir. F5’in varlığı, bakteri izolatının Enterotoksijenik E. coli olarak sınıflandırılmasını sağlar. Arı zehiri ve arı zehrinden izole edilen eksozomlar, antimikrobiyal ve antibiyofilm aktivite sergileyen bioaktif bileşiklerdir. Bu çalışmanın amacı buzağı ishaline sebep olan E. coli,’ye karşı arı zehiri ve arı zehrinden izole edilen eksozomların antimikrobiyal ve antibiyofilm aktivitesini ortaya koymaktır. E. coli suşlarına karşı hem eksozom hem de arı zehirinin minimum inhibisyon konsantrasyonu (MIC), antibiyofilm aktivitesi ve fraksiyonel inhibisyon konsantrasyonları (FIC) ve L929 hücrelerinde canlılık oranları belirlendi. İmmünohistokimyasal olarak hücre hasarı floresan mikroskop altında incelendi. Çalışmamızda arı zehirinden izole edilen eksozomların MIC değeri 1.95 μg/mL olarak tespit edildi. Amoksisilin klavulonik asit ile yapılan kombinasyonlarda 0.44 değer ile sinerjik etki tespit edildi. Antibiyofilm aktivitesi arı zehrinde %48.8 oranında belirlenirken arı zehri eksozomu % 60.4 oranında biyofilm tabakasını inhibe ettiği tespit edildi. L929 hücre hatlarında kombinasyon grupların canlılık oranını düşürdüğü rapor edildi. Arı zehri eksozomları arı zehrinden daha fazla bakteriler üzerinde etkili olmaktadır. Sonuç olarak; biyouyumlu molekül olan ve kargo elemanı olarak görev yapan arı zehiri eksozomunun antimikrobiyal ve özellikle antibiyofilm aktivite sergilemesi artan antibiyotik direncine karşı alternatif bir yaklaşım olması önem arz etmektedir.
Anahtar Kelime: Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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| APA | ÇELEBİ D, ÇELEBİ Ö, Baser S, TAGHİZADEHGHALEHJOUGHİ A (2023). Evaluation of Antimicrobial and Antibiofilm Efficacy of Bee Venom and Exosome Against Escherichia coli K99 Strain. , 239 - 246. 10.9775/kvfd.2023.29132 |
| Chicago | ÇELEBİ Demet,ÇELEBİ Özgür,Baser Sümeyye,TAGHİZADEHGHALEHJOUGHİ Ali Evaluation of Antimicrobial and Antibiofilm Efficacy of Bee Venom and Exosome Against Escherichia coli K99 Strain. (2023): 239 - 246. 10.9775/kvfd.2023.29132 |
| MLA | ÇELEBİ Demet,ÇELEBİ Özgür,Baser Sümeyye,TAGHİZADEHGHALEHJOUGHİ Ali Evaluation of Antimicrobial and Antibiofilm Efficacy of Bee Venom and Exosome Against Escherichia coli K99 Strain. , 2023, ss.239 - 246. 10.9775/kvfd.2023.29132 |
| AMA | ÇELEBİ D,ÇELEBİ Ö,Baser S,TAGHİZADEHGHALEHJOUGHİ A Evaluation of Antimicrobial and Antibiofilm Efficacy of Bee Venom and Exosome Against Escherichia coli K99 Strain. . 2023; 239 - 246. 10.9775/kvfd.2023.29132 |
| Vancouver | ÇELEBİ D,ÇELEBİ Ö,Baser S,TAGHİZADEHGHALEHJOUGHİ A Evaluation of Antimicrobial and Antibiofilm Efficacy of Bee Venom and Exosome Against Escherichia coli K99 Strain. . 2023; 239 - 246. 10.9775/kvfd.2023.29132 |
| IEEE | ÇELEBİ D,ÇELEBİ Ö,Baser S,TAGHİZADEHGHALEHJOUGHİ A "Evaluation of Antimicrobial and Antibiofilm Efficacy of Bee Venom and Exosome Against Escherichia coli K99 Strain." , ss.239 - 246, 2023. 10.9775/kvfd.2023.29132 |
| ISNAD | ÇELEBİ, Demet vd. "Evaluation of Antimicrobial and Antibiofilm Efficacy of Bee Venom and Exosome Against Escherichia coli K99 Strain". (2023), 239-246. https://doi.org/10.9775/kvfd.2023.29132 |
| APA | ÇELEBİ D, ÇELEBİ Ö, Baser S, TAGHİZADEHGHALEHJOUGHİ A (2023). Evaluation of Antimicrobial and Antibiofilm Efficacy of Bee Venom and Exosome Against Escherichia coli K99 Strain. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 29(3), 239 - 246. 10.9775/kvfd.2023.29132 |
| Chicago | ÇELEBİ Demet,ÇELEBİ Özgür,Baser Sümeyye,TAGHİZADEHGHALEHJOUGHİ Ali Evaluation of Antimicrobial and Antibiofilm Efficacy of Bee Venom and Exosome Against Escherichia coli K99 Strain. Kafkas Üniversitesi Veteriner Fakültesi Dergisi 29, no.3 (2023): 239 - 246. 10.9775/kvfd.2023.29132 |
| MLA | ÇELEBİ Demet,ÇELEBİ Özgür,Baser Sümeyye,TAGHİZADEHGHALEHJOUGHİ Ali Evaluation of Antimicrobial and Antibiofilm Efficacy of Bee Venom and Exosome Against Escherichia coli K99 Strain. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, vol.29, no.3, 2023, ss.239 - 246. 10.9775/kvfd.2023.29132 |
| AMA | ÇELEBİ D,ÇELEBİ Ö,Baser S,TAGHİZADEHGHALEHJOUGHİ A Evaluation of Antimicrobial and Antibiofilm Efficacy of Bee Venom and Exosome Against Escherichia coli K99 Strain. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 2023; 29(3): 239 - 246. 10.9775/kvfd.2023.29132 |
| Vancouver | ÇELEBİ D,ÇELEBİ Ö,Baser S,TAGHİZADEHGHALEHJOUGHİ A Evaluation of Antimicrobial and Antibiofilm Efficacy of Bee Venom and Exosome Against Escherichia coli K99 Strain. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 2023; 29(3): 239 - 246. 10.9775/kvfd.2023.29132 |
| IEEE | ÇELEBİ D,ÇELEBİ Ö,Baser S,TAGHİZADEHGHALEHJOUGHİ A "Evaluation of Antimicrobial and Antibiofilm Efficacy of Bee Venom and Exosome Against Escherichia coli K99 Strain." Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 29, ss.239 - 246, 2023. 10.9775/kvfd.2023.29132 |
| ISNAD | ÇELEBİ, Demet vd. "Evaluation of Antimicrobial and Antibiofilm Efficacy of Bee Venom and Exosome Against Escherichia coli K99 Strain". Kafkas Üniversitesi Veteriner Fakültesi Dergisi 29/3 (2023), 239-246. https://doi.org/10.9775/kvfd.2023.29132 |
