Hydroxychloroquine induces oxidative stress and impairs fracture healing in rats

Doğar, Fatih; bilal, ökkeş; EKEN, AYSE; TOPAK, DURAN; Gürbüz, Kaan; BAKIR, ELÇIN; Gürbüz, Perihan; KILINÇ, Emine

doi:10.52312/jdrs.2023.976

Hydroxychloroquine induces oxidative stress and impairs fracture healing in rats

Duran TOPAK, (Kahramanmaraş Sütçü İmam Üniversitesi, Tıp Fakültesi, Ortopedi ve Travmatoloji Anabilim Dalı, Kahramanmaraş, Türkiye)

Kaan GÜRBÜZ, (Kayseri Şehir Eğitim ve Araştırma Hastanesi, Ortopedi ve Travmatoloji Anabilim Dalı, Kayseri, Türkiye)

Fatih DOĞAR, (Kahramanmaraş Sütçü İmam Üniversitesi, Tıp Fakültesi, Ortopedi ve Travmatoloji Anabilim Dalı, Kahramanmaraş, Türkiye)

Elçin BAKIR, (Erciyes Üniversitesi, Eczacılık Fakültesi, Farmasötik Toksikoloji Anabilim Dalı, Kayseri, Türkiye)

Perihan GÜRBÜZ, (Erciyes Üniversitesi, Eczacılık Fakültesi, Farmakognozi Anabilim Dalı, Kayseri, Türkiye)

Emine KILINÇ, (Kahramanmaraş Sütçü İmam Üniversitesi, Tıp Fakültesi, Patoloji Anabilim Dalı, Kahramanmaraş, Türkiye)

Ökkeş BİLAL, (Kahramanmaraş Sütçü İmam Üniversitesi, Tıp Fakültesi, Ortopedi ve Travmatoloji Anabilim Dalı, Kahramanmaraş, Türkiye)

Ayşe EKEN (Erciyes Üniversitesi, Eczacılık Fakültesi, Farmasötik Toksikoloji Anabilim Dalı, Kayseri, Türkiye)

Joint diseases and related surgery

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Yıl: 2023 Cilt: 34 Sayı: 2 Sayfa Aralığı: 346 - 355 Metin Dili: İngilizce DOI: 10.52312/jdrs.2023.976 İndeks Tarihi: 11-07-2023

Hydroxychloroquine induces oxidative stress and impairs fracture healing in rats

Öz:

Objectives: The aim of this study was to investigate whether hydroxychloroquine sulfate (HCQS) induced oxidative stress and how it affected the union of bone fractures in an experimental rat model. Materials and methods: A total of 48 Wistar albino male rats were used. The rats were divided into six groups. To investigate the effects of oral administration of HCQS at varying doses between the third and sixth weeks, fracture healing processes were evaluated using radiography, histopathology, biochemistry, and dual-energy X-ray absorptiometry. The activities of antioxidant enzymes superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), and malondialdehyde (MDA) were measured to analyze the relationship between HCQS and oxidative stress. Results: Radiographic scores, alkaline phosphatase levels, callus/diaphysis ratio, callus development, and bone mineral density were significantly lower in rats given HCQS at three and six weeks compared to the control group (p<0.005). When oxidative stress parameters were compared among the groups, all antioxidant parameters were statistically significant, indicating that antioxidant systems played a role in peripheral blood, when HCQS was used (p<0.005). Conclusion: Oral HCQS intake impairs the fracture healing process by causing oxidative stress in rats. However, further biomolecular researches are needed to understand the underlying mechanism of these effects.

Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	TOPAK D, Gürbüz K, Doğar F, BAKIR E, Gürbüz P, KILINÇ E, bilal ö, EKEN A (2023). Hydroxychloroquine induces oxidative stress and impairs fracture healing in rats. , 346 - 355. 10.52312/jdrs.2023.976
Chicago	TOPAK DURAN,Gürbüz Kaan,Doğar Fatih,BAKIR ELÇIN,Gürbüz Perihan,KILINÇ Emine,bilal ökkeş,EKEN AYSE Hydroxychloroquine induces oxidative stress and impairs fracture healing in rats. (2023): 346 - 355. 10.52312/jdrs.2023.976
MLA	TOPAK DURAN,Gürbüz Kaan,Doğar Fatih,BAKIR ELÇIN,Gürbüz Perihan,KILINÇ Emine,bilal ökkeş,EKEN AYSE Hydroxychloroquine induces oxidative stress and impairs fracture healing in rats. , 2023, ss.346 - 355. 10.52312/jdrs.2023.976
AMA	TOPAK D,Gürbüz K,Doğar F,BAKIR E,Gürbüz P,KILINÇ E,bilal ö,EKEN A Hydroxychloroquine induces oxidative stress and impairs fracture healing in rats. . 2023; 346 - 355. 10.52312/jdrs.2023.976
Vancouver	TOPAK D,Gürbüz K,Doğar F,BAKIR E,Gürbüz P,KILINÇ E,bilal ö,EKEN A Hydroxychloroquine induces oxidative stress and impairs fracture healing in rats. . 2023; 346 - 355. 10.52312/jdrs.2023.976
IEEE	TOPAK D,Gürbüz K,Doğar F,BAKIR E,Gürbüz P,KILINÇ E,bilal ö,EKEN A "Hydroxychloroquine induces oxidative stress and impairs fracture healing in rats." , ss.346 - 355, 2023. 10.52312/jdrs.2023.976
ISNAD	TOPAK, DURAN vd. "Hydroxychloroquine induces oxidative stress and impairs fracture healing in rats". (2023), 346-355. https://doi.org/10.52312/jdrs.2023.976

APA	TOPAK D, Gürbüz K, Doğar F, BAKIR E, Gürbüz P, KILINÇ E, bilal ö, EKEN A (2023). Hydroxychloroquine induces oxidative stress and impairs fracture healing in rats. Joint diseases and related surgery, 34(2), 346 - 355. 10.52312/jdrs.2023.976
Chicago	TOPAK DURAN,Gürbüz Kaan,Doğar Fatih,BAKIR ELÇIN,Gürbüz Perihan,KILINÇ Emine,bilal ökkeş,EKEN AYSE Hydroxychloroquine induces oxidative stress and impairs fracture healing in rats. Joint diseases and related surgery 34, no.2 (2023): 346 - 355. 10.52312/jdrs.2023.976
MLA	TOPAK DURAN,Gürbüz Kaan,Doğar Fatih,BAKIR ELÇIN,Gürbüz Perihan,KILINÇ Emine,bilal ökkeş,EKEN AYSE Hydroxychloroquine induces oxidative stress and impairs fracture healing in rats. Joint diseases and related surgery, vol.34, no.2, 2023, ss.346 - 355. 10.52312/jdrs.2023.976
AMA	TOPAK D,Gürbüz K,Doğar F,BAKIR E,Gürbüz P,KILINÇ E,bilal ö,EKEN A Hydroxychloroquine induces oxidative stress and impairs fracture healing in rats. Joint diseases and related surgery. 2023; 34(2): 346 - 355. 10.52312/jdrs.2023.976
Vancouver	TOPAK D,Gürbüz K,Doğar F,BAKIR E,Gürbüz P,KILINÇ E,bilal ö,EKEN A Hydroxychloroquine induces oxidative stress and impairs fracture healing in rats. Joint diseases and related surgery. 2023; 34(2): 346 - 355. 10.52312/jdrs.2023.976
IEEE	TOPAK D,Gürbüz K,Doğar F,BAKIR E,Gürbüz P,KILINÇ E,bilal ö,EKEN A "Hydroxychloroquine induces oxidative stress and impairs fracture healing in rats." Joint diseases and related surgery, 34, ss.346 - 355, 2023. 10.52312/jdrs.2023.976
ISNAD	TOPAK, DURAN vd. "Hydroxychloroquine induces oxidative stress and impairs fracture healing in rats". Joint diseases and related surgery 34/2 (2023), 346-355. https://doi.org/10.52312/jdrs.2023.976