Investigation of The Antiproliferative Effect of Colchicine on SNU-1 Gastric Cancer Cells

yulak, fatih

doi:10.17776/csj.1256954

Investigation of The Antiproliferative Effect of Colchicine on SNU-1 Gastric Cancer Cells

Fatih YULAK (Sivas Cumhuriyet Üniversitesi, Tıp Fakültesi, Fizyoloji Anabilim Dalı, Sivas, Türkiye)

Cumhuriyet Science Journal

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Yıl: 2023 Cilt: 44 Sayı: 2 Sayfa Aralığı: 263 - 267 Metin Dili: İngilizce DOI: 10.17776/csj.1256954 İndeks Tarihi: 12-07-2023

Investigation of The Antiproliferative Effect of Colchicine on SNU-1 Gastric Cancer Cells

Öz:

In this study, colchicine's cytotoxic effects on SNU-1 cells were examined, and a probable mechanism behind its cytotoxicity was revealed. According to the results of the study, colchicine displayed considerable cytotoxicity with an IC50 value of 14.81ng/ml when it was administered to the cells for 24 hours at different doses ranging from 5 to 100ng/ml. Furthermore, according to mechanistic studies, usege of colchicine significantly increased both early and late apoptotic cells in flow cytometry experiments. The late apoptotic cell population percentage in the control group (5.14 ± 1.27%) dramatically increased to 22.83 ± 1.38% in 14.81ng/ml colchicine treated cells. The early apoptotic cell population percentage in the control group (2.00 ± 1.12%) increased to 6.57 ± 2.35% in 14.81ng/ml colchicine treated cells. ELISA method was used to evaluate how colchicine affects the expression of pro- and anti-apoptotic proteins in SNU-1 cells. Colchicine treatment increased pro-apoptotic Bax and cleaved caspase 3 activities, while anti-apoptotic BCL-2 levels decreased. It is concluded that colchicine increases apoptosis in SNU-1 cells, which leads to an overall increase in cell death. Colchicine's promise as an anticancer drug to treat stomach cancer, however, needs additional research to be determined.

Anahtar Kelime: Gastric cancer Colchicine Antiproliferative effect Apoptozis.

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	yulak f (2023). Investigation of The Antiproliferative Effect of Colchicine on SNU-1 Gastric Cancer Cells. , 263 - 267. 10.17776/csj.1256954
Chicago	yulak fatih Investigation of The Antiproliferative Effect of Colchicine on SNU-1 Gastric Cancer Cells. (2023): 263 - 267. 10.17776/csj.1256954
MLA	yulak fatih Investigation of The Antiproliferative Effect of Colchicine on SNU-1 Gastric Cancer Cells. , 2023, ss.263 - 267. 10.17776/csj.1256954
AMA	yulak f Investigation of The Antiproliferative Effect of Colchicine on SNU-1 Gastric Cancer Cells. . 2023; 263 - 267. 10.17776/csj.1256954
Vancouver	yulak f Investigation of The Antiproliferative Effect of Colchicine on SNU-1 Gastric Cancer Cells. . 2023; 263 - 267. 10.17776/csj.1256954
IEEE	yulak f "Investigation of The Antiproliferative Effect of Colchicine on SNU-1 Gastric Cancer Cells." , ss.263 - 267, 2023. 10.17776/csj.1256954
ISNAD	yulak, fatih. "Investigation of The Antiproliferative Effect of Colchicine on SNU-1 Gastric Cancer Cells". (2023), 263-267. https://doi.org/10.17776/csj.1256954

APA	yulak f (2023). Investigation of The Antiproliferative Effect of Colchicine on SNU-1 Gastric Cancer Cells. Cumhuriyet Science Journal, 44(2), 263 - 267. 10.17776/csj.1256954
Chicago	yulak fatih Investigation of The Antiproliferative Effect of Colchicine on SNU-1 Gastric Cancer Cells. Cumhuriyet Science Journal 44, no.2 (2023): 263 - 267. 10.17776/csj.1256954
MLA	yulak fatih Investigation of The Antiproliferative Effect of Colchicine on SNU-1 Gastric Cancer Cells. Cumhuriyet Science Journal, vol.44, no.2, 2023, ss.263 - 267. 10.17776/csj.1256954
AMA	yulak f Investigation of The Antiproliferative Effect of Colchicine on SNU-1 Gastric Cancer Cells. Cumhuriyet Science Journal. 2023; 44(2): 263 - 267. 10.17776/csj.1256954
Vancouver	yulak f Investigation of The Antiproliferative Effect of Colchicine on SNU-1 Gastric Cancer Cells. Cumhuriyet Science Journal. 2023; 44(2): 263 - 267. 10.17776/csj.1256954
IEEE	yulak f "Investigation of The Antiproliferative Effect of Colchicine on SNU-1 Gastric Cancer Cells." Cumhuriyet Science Journal, 44, ss.263 - 267, 2023. 10.17776/csj.1256954
ISNAD	yulak, fatih. "Investigation of The Antiproliferative Effect of Colchicine on SNU-1 Gastric Cancer Cells". Cumhuriyet Science Journal 44/2 (2023), 263-267. https://doi.org/10.17776/csj.1256954