Introduction e present study aimed to look into any potential ameliorative bene ts of boric acid on liver damage in rats caused by cyclophosphamide (CTX). Materials and Methods Four groups, control, boric acid, CTX, and boric acid+CTX, were created. Female Wistar albino rats were given daily injections of CTX (75 mg/kg) to create the liver damage model. Cyclophosphamide (75 mg/kg) was administered intraperitoneally, and boron (1.3 g/rat/day) was administered by gavage every day for two weeks in the boric acid+CTX group. e histopathological changes were evaluated in liver tissue staining with hematoxylin and eosin, Masson trichrome, and periodic acid Schiff. In addition, we assessed liver tissue enzyme activity as malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px). Results Images in the boric acid+CTX group had lower histological evaluations than those in the CTX group under the light microscope. According to the ndings, boric acid reduced MDA levels in the liver tissues. Additionally, boric acid improved the actions of oxidative stress indicators to reduce oxidative stress brought on by CTX and upregulated antioxidant parameters. Conclusion In conclusion, our study has demonstrated that CTX- induced liver injury can be alleviated by reducing the tissue MDA levels, and increasing the liver’s SOD, GSH-Px, and CAT activities. In order to reduce the liver damage caused by CTX, boric acid may be administered as a dietary supplement or functional food.