Effects of Ozone Threapy as an Adjunct to Non-Surgical Periodontal Treatment on Clinical Periodontal Parameters and Inflammatory Markers in Periodontitis Patients
Yıl: 2023 Cilt: 24 Sayı: 2 Sayfa Aralığı: 119 - 124 Metin Dili: İngilizce DOI: 10.4274/meandros.galenos.2021.90532 İndeks Tarihi: 26-07-2023
Effects of Ozone Threapy as an Adjunct to Non-Surgical Periodontal Treatment on Clinical Periodontal Parameters and Inflammatory Markers in Periodontitis Patients
Öz: Objective: Ozone therapy (OT) is known to eliminate pathogens in the periodontal pocket and accelerate wound healing. This study aimed to investigate the clinical and biochemical efficacy of OT as adjunctive scaling and root planning (SRP) in the treatment of periodontitis. Materials and Methods: Fifty systemically healthy individuals with periodontitis were included in this study. Gingival crevicular fluid (GCF) samples were taken from the patients before and 8 weeks after starting the treatment, and gingival index (GI), plaque index (PI), bleeding on probing (BOP), pocket depth (PD), and clinical attachment level (CAL) were obtained from all subjects. Non-surgical periodontal treatment was applied to the test and control groups, and gaseous ozone treatment was applied in addition to the test group. TNF-α, IL-1β, IL-6, IL-10, and MMP-9 values in GCF samples before and after treatment were determined by the ELISA method and evaluated statistically. Results: Both groups presented significantly decreased clinical periodontal parameters at 8-week from baseline (p<0.05). Clinical periodontal parameters were found to be significantly lower in the test group than In the control group, there was a decrease in TNF-α levels after non-surgical periodontal treatment (p<0.05). Both groups demonstrated significantly decreased IL-1β, IL-6, IL-10, and MMP-9 levels at 8-week (p<0.05). The test group showed a significant decrease in MMP-9 levels compared with the control group at 8-week (p<0.05). Conclusion: As a result of the close relationship observed between clinical periodontal measurements and biochemical parameters, it was observed that clinical periodontal healing affects biochemical parameters, and in addition to non-surgical periodontal treatment, ozone treatment improves clinical periodontal measurements and affects biochemical parameters by reducing the GCF volume.
Anahtar Kelime: Periodontitisli Bireylerde Cerrahi Olmayan Periodontal Tedaviye Ek Olarak Uygulanan Ozonun Klinik Periodontal Parametrelere ve Enflamatuvar Belirteç Seviyelerine Etkileri
Öz: Amaç: Medikal ozon tedavisinin, periodontal cepteki patojenleri elimine ettiği ve yara iyileşmesini hızlandırdığı bilinmektedir. Bu çalışmanın amacı, periodontal hastalıklı bireylerde ozon tedavisinin klinik ve biyokimyasal olarak etkilerinin değerlendirilmesidir. Gereç ve Yöntemler: Bu çalışmaya sistemik olarak sağlıklı ve periodontitisli 50 birey dahil edildi. Tedaviye başlamadan önce ve 8 hafta sonra hastalardan dişeti oluğu sıvısı (DOS) örnekleri alınmış, tüm bireylerden gingival indeks, plak indeksi, sondalamada kanama, sondalamada cep derinliği ve klinik ataçman seviyesini içeren klinik periodontal ölçümler elde edilmiştir. Test ve kontrol grubuna cerrahi olmayan periodontal tedavi yapılmış, test grubuna ek olarak gaz ozon tedavisi uygulanmıştır. Tedavi öncesi ve sonrası, DOS örneklerinde Tumor necrosis factor-α (TNF-α), IL-1β, IL-6, IL-10 ve MMP-9 değerleri ELİSA metoduyla saptanmış ve istatistiksel olarak değerlendirilmiştir. Bulgular: Hem test hem de kontrol grubunda cerrahi olmayan periodontal tedavi sonrası klinik periodontal ölçümlerde başlangıç değerlerine göre azalma saptanmıştır (p<0,05). Test grubunda, kontrol grubuna göre, klinik periodontal ölçümlerde azalma tespit edilmiştir (p<0,05). Her iki grupta da DOS IL-1β, IL-6, IL-10 ve MMP-9 seviyelerinde başlangıç değerlerine göre, cerrahi olmayan periodontal tedavi sonrası azalma gözlenmiştir (p<0,05). Kontrol grubunda DOS TNF-α seviyesinde başlangıç değerlerine göre, cerrahi olmayan periodontal tedavi sonrasında azalma saptanmıştır (p<0,05). Test grubunda kontrol grubuna göre, DOS MMP-9 seviyesinde azalma gözlenmiştir (p<0,05). Sonuç: Klinik periodontal ölçümler ve biyokimyasal parametreler arasında gözlenen yakın ilişki, klinik periodontal iyileşmenin biyokimyasal parametreleri etkilediği, cerrahi olmayan periodontal tedaviye ek olarak ozon tedavisinin DOS hacmini azaltarak klinik periodontal ölçümleri iyileştirdiği ve biyokimyasal parametrelere etki ettiği de gözlenmiştir.
Anahtar Kelime: Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
- 1. Armitage GC. Development of a classification system for periodontal diseases and conditions. Ann Periodontol 1999; 4: 1-6.
- 2. Aspriello SD, Piemontese M, Levrini L, Sauro S. Ultramorphology of the root surface subsequent to hand-ultrasonic simultaneous instrumentation during non-surgical periodontal treatments: an in vitro study. J Appl Oral Sci 2011; 19: 74-81.
- 3. Quirynen M, Teughels W, De Soete M, van Steenberghe D. Topical antiseptics and antibiotics in the initial therapy of chronic adult periodontitis: microbiological aspects. Periodontol 2000 2002; 28: 72-90.
- 4. Yılmaz S, Algan S, Gursoy H, Noyan U, Kuru BE, Kadir T. Evaluation of the clinical and antimicrobial effects of the Er:YAG laser or topical gaseous ozone as adjuncts to initial periodontal therapy. Photomed Laser Surg 2013; 31: 293-8.
- 5. Eick S, Tigan M, Sculean A. Effect of ozone on periodontopathogenic species--an in vitro study. Clin Oral Investig 2012; 16: 537-44.
- 6. Bocci VA. Scientific and medical aspects of ozone therapy. State of the art. Arch Med Res 2006; 37: 425-35.
- 7. Saglam E, Alinca SB, Celik TZ, Hacisalihoglu UP, Dogan MA. Evaluation of the effect of topical and systemic ozone application in periodontitis: an experimental study in rats. J Appl Oral Sci 2019; 28: e20190140.
- 8. Seydanur Dengizek E, Serkan D, Abubekir E, Aysun Bay K, Onder O, Arife C. Evaluating clinical and laboratory effects of ozone in non-surgical periodontal treatment: a randomized controlled trial. J Appl Oral Sci 2019; 27: e20180108.
- 9. Hayakumo S, Arakawa S, Mano Y, Izumi Y. Clinical and microbiological effects of ozone nano-bubble water irrigation as an adjunct to mechanical subgingival debridement in periodontitis patients in a randomized controlled trial. Clin Oral Investig 2013; 17: 379-88.
- 10. Ranjith A, Niranjana JM, Baiju KV. Adjunctive benefit of ozonized water irrigation with mechanical debridement in the management of Stage III periodontitis: A randomized controlled clinical and biochemical study. Int J Dent Hyg 2022; 20: 364-70.
- 11. Caton JG, Armitage G, Berglundh T, Chapple ILC, Jepsen S, Kornman KS, et al. A new classification scheme for periodontal and peri-implant diseases and conditions - Introduction and key changes from the 1999 classification. J Periodontol 2018; 89 Suppl 1: S1-8.
- 12. Kshitish D, Laxman VK. The use of ozonated water and 0.2% chlorhexidine in the treatment of periodontitis patients: a clinical and microbiologic study. Indian J Dent Res 2010; 21: 341-8.
- 13. Katti SS, Chava VK. Effect of Ozonised water on Chronic Periodontitis - A Clinical Study. J Int Oral Health 2013; 5: 79-84.
- 14. Skurska A, Pietruska MD, Paniczko-Drężek A, et al. Evaluation of the influence of ozonotherapy on the clinical parameters and MMP levels in patients with chronic and aggressive periodontitis. Adv Med Sci 2010; 55: 297-307.
- 15. Uraz A, Karaduman B, Isler SÇ, Gönen S, Çetiner D. Ozone application as adjunctive therapy in chronic periodontitis: Clinical, microbiological and biochemical aspects. J Dent Sci 2019; 14: 27- 37.
- 16. Tasdemir Z, Oskaybas MN, Alkan AB, Cakmak O. The effects of ozone therapy on periodontal therapy: A randomized placebo- controlled clinical trial. Oral Dis 2019; 25: 1195-202.
- 17. Al Habashneh R, Alsalman W, Khader Y. Ozone as an adjunct to conventional nonsurgical therapy in chronic periodontitis: a randomized controlled clinical trial. J Periodontal Res 2015; 50: 37- 43.
- 18. Bocci V. Is it true that ozone is always toxic? The end of a dogma. Toxicol Appl Pharmacol 2006; 216: 493-504.
- 19. Holmes J. Clinical reversal of root caries using ozone, double-blind, randomised, controlled 18-month trial. Gerodontology 2003; 20: 106-14.
- 20. Bocci V. Ozone as a bioregulator. Pharmacology and toxicology of ozonetherapy today. J Biol Regul Homeost Agents 1996; 10: 31-53.
| APA | Sevinç Gül S, Dilsiz A, Ozturk N, Gül M (2023). Effects of Ozone Threapy as an Adjunct to Non-Surgical Periodontal Treatment on Clinical Periodontal Parameters and Inflammatory Markers in Periodontitis Patients. , 119 - 124. 10.4274/meandros.galenos.2021.90532 |
| Chicago | Sevinç Gül Sema Nur,Dilsiz Alparslan,Ozturk Nurinnisa,Gül Mehmet Ali Effects of Ozone Threapy as an Adjunct to Non-Surgical Periodontal Treatment on Clinical Periodontal Parameters and Inflammatory Markers in Periodontitis Patients. (2023): 119 - 124. 10.4274/meandros.galenos.2021.90532 |
| MLA | Sevinç Gül Sema Nur,Dilsiz Alparslan,Ozturk Nurinnisa,Gül Mehmet Ali Effects of Ozone Threapy as an Adjunct to Non-Surgical Periodontal Treatment on Clinical Periodontal Parameters and Inflammatory Markers in Periodontitis Patients. , 2023, ss.119 - 124. 10.4274/meandros.galenos.2021.90532 |
| AMA | Sevinç Gül S,Dilsiz A,Ozturk N,Gül M Effects of Ozone Threapy as an Adjunct to Non-Surgical Periodontal Treatment on Clinical Periodontal Parameters and Inflammatory Markers in Periodontitis Patients. . 2023; 119 - 124. 10.4274/meandros.galenos.2021.90532 |
| Vancouver | Sevinç Gül S,Dilsiz A,Ozturk N,Gül M Effects of Ozone Threapy as an Adjunct to Non-Surgical Periodontal Treatment on Clinical Periodontal Parameters and Inflammatory Markers in Periodontitis Patients. . 2023; 119 - 124. 10.4274/meandros.galenos.2021.90532 |
| IEEE | Sevinç Gül S,Dilsiz A,Ozturk N,Gül M "Effects of Ozone Threapy as an Adjunct to Non-Surgical Periodontal Treatment on Clinical Periodontal Parameters and Inflammatory Markers in Periodontitis Patients." , ss.119 - 124, 2023. 10.4274/meandros.galenos.2021.90532 |
| ISNAD | Sevinç Gül, Sema Nur vd. "Effects of Ozone Threapy as an Adjunct to Non-Surgical Periodontal Treatment on Clinical Periodontal Parameters and Inflammatory Markers in Periodontitis Patients". (2023), 119-124. https://doi.org/10.4274/meandros.galenos.2021.90532 |
| APA | Sevinç Gül S, Dilsiz A, Ozturk N, Gül M (2023). Effects of Ozone Threapy as an Adjunct to Non-Surgical Periodontal Treatment on Clinical Periodontal Parameters and Inflammatory Markers in Periodontitis Patients. Meandros Medical And Dental Journal, 24(2), 119 - 124. 10.4274/meandros.galenos.2021.90532 |
| Chicago | Sevinç Gül Sema Nur,Dilsiz Alparslan,Ozturk Nurinnisa,Gül Mehmet Ali Effects of Ozone Threapy as an Adjunct to Non-Surgical Periodontal Treatment on Clinical Periodontal Parameters and Inflammatory Markers in Periodontitis Patients. Meandros Medical And Dental Journal 24, no.2 (2023): 119 - 124. 10.4274/meandros.galenos.2021.90532 |
| MLA | Sevinç Gül Sema Nur,Dilsiz Alparslan,Ozturk Nurinnisa,Gül Mehmet Ali Effects of Ozone Threapy as an Adjunct to Non-Surgical Periodontal Treatment on Clinical Periodontal Parameters and Inflammatory Markers in Periodontitis Patients. Meandros Medical And Dental Journal, vol.24, no.2, 2023, ss.119 - 124. 10.4274/meandros.galenos.2021.90532 |
| AMA | Sevinç Gül S,Dilsiz A,Ozturk N,Gül M Effects of Ozone Threapy as an Adjunct to Non-Surgical Periodontal Treatment on Clinical Periodontal Parameters and Inflammatory Markers in Periodontitis Patients. Meandros Medical And Dental Journal. 2023; 24(2): 119 - 124. 10.4274/meandros.galenos.2021.90532 |
| Vancouver | Sevinç Gül S,Dilsiz A,Ozturk N,Gül M Effects of Ozone Threapy as an Adjunct to Non-Surgical Periodontal Treatment on Clinical Periodontal Parameters and Inflammatory Markers in Periodontitis Patients. Meandros Medical And Dental Journal. 2023; 24(2): 119 - 124. 10.4274/meandros.galenos.2021.90532 |
| IEEE | Sevinç Gül S,Dilsiz A,Ozturk N,Gül M "Effects of Ozone Threapy as an Adjunct to Non-Surgical Periodontal Treatment on Clinical Periodontal Parameters and Inflammatory Markers in Periodontitis Patients." Meandros Medical And Dental Journal, 24, ss.119 - 124, 2023. 10.4274/meandros.galenos.2021.90532 |
| ISNAD | Sevinç Gül, Sema Nur vd. "Effects of Ozone Threapy as an Adjunct to Non-Surgical Periodontal Treatment on Clinical Periodontal Parameters and Inflammatory Markers in Periodontitis Patients". Meandros Medical And Dental Journal 24/2 (2023), 119-124. https://doi.org/10.4274/meandros.galenos.2021.90532 |
