Investigation the role of nitric oxide pathway in TNF-α induced HUVEC cells
Yıl: 2023 Cilt: 27 Sayı: 4 Sayfa Aralığı: 1560 - 1566 Metin Dili: İngilizce DOI: 10.29228/jrp.441 İndeks Tarihi: 28-07-2023
Investigation the role of nitric oxide pathway in TNF-α induced HUVEC cells
Öz: Nitric oxide (NO) is a highly reactive molecule involved in a variety of physiological and pathophysiological processes, such as inflammation. eNOS-produced NO has anti-inflammatory properties and play a role in vascular homeostasis. Through a variety of mechanisms, tumor necrosis factor alpha (TNF-α) has been shown to induce inflammation in HUVECs. The purpose of this study was to investigate the role of NO in HUVEC cells under inflammatory conditions induced by TNF-α. To identify TNF-α induction mechanisms, the phosphorylation of ERK, Akt, and eNOS was investigated. Sildenafil and L-NAME used to examine the role of NO in this process. In TNF-α- induced HUVEC cells, cell viability, nitrite/nitrate production, phosphorylated and total ERK, Akt, and eNOS levels were measured with or without sildenafil and L-NAME. At 20 and 40 ng/ml concentrations, TNF-α increased nitrite/nitrate and decreased cell viability (p<0.05). Sildenafil and L-NAME had no effect on cell viability and they both decreased nitrite/ nitrate in HUVEC that had been stimulated by TNF-α. TNF-α had no effect on the phosphorylation of ERK, Akt, and eNOS, but it did increase total eNOS levels. The phosphorylation of ERK, Akt, and eNOS was unaffected by sildenafil and L-NAME; however, L-NAME decreased total eNOS. According to our findings, there is no known direct relationship between TNF-α, sildenafil, or L-NAME and protein phosphorylation.
Anahtar Kelime: Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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| APA | CIVELEK E, CIVELEK D (2023). Investigation the role of nitric oxide pathway in TNF-α induced HUVEC cells. , 1560 - 1566. 10.29228/jrp.441 |
| Chicago | CIVELEK Erkan,CIVELEK Dilek OZTURK Investigation the role of nitric oxide pathway in TNF-α induced HUVEC cells. (2023): 1560 - 1566. 10.29228/jrp.441 |
| MLA | CIVELEK Erkan,CIVELEK Dilek OZTURK Investigation the role of nitric oxide pathway in TNF-α induced HUVEC cells. , 2023, ss.1560 - 1566. 10.29228/jrp.441 |
| AMA | CIVELEK E,CIVELEK D Investigation the role of nitric oxide pathway in TNF-α induced HUVEC cells. . 2023; 1560 - 1566. 10.29228/jrp.441 |
| Vancouver | CIVELEK E,CIVELEK D Investigation the role of nitric oxide pathway in TNF-α induced HUVEC cells. . 2023; 1560 - 1566. 10.29228/jrp.441 |
| IEEE | CIVELEK E,CIVELEK D "Investigation the role of nitric oxide pathway in TNF-α induced HUVEC cells." , ss.1560 - 1566, 2023. 10.29228/jrp.441 |
| ISNAD | CIVELEK, Erkan - CIVELEK, Dilek OZTURK. "Investigation the role of nitric oxide pathway in TNF-α induced HUVEC cells". (2023), 1560-1566. https://doi.org/10.29228/jrp.441 |
| APA | CIVELEK E, CIVELEK D (2023). Investigation the role of nitric oxide pathway in TNF-α induced HUVEC cells. Journal of research in pharmacy (online), 27(4), 1560 - 1566. 10.29228/jrp.441 |
| Chicago | CIVELEK Erkan,CIVELEK Dilek OZTURK Investigation the role of nitric oxide pathway in TNF-α induced HUVEC cells. Journal of research in pharmacy (online) 27, no.4 (2023): 1560 - 1566. 10.29228/jrp.441 |
| MLA | CIVELEK Erkan,CIVELEK Dilek OZTURK Investigation the role of nitric oxide pathway in TNF-α induced HUVEC cells. Journal of research in pharmacy (online), vol.27, no.4, 2023, ss.1560 - 1566. 10.29228/jrp.441 |
| AMA | CIVELEK E,CIVELEK D Investigation the role of nitric oxide pathway in TNF-α induced HUVEC cells. Journal of research in pharmacy (online). 2023; 27(4): 1560 - 1566. 10.29228/jrp.441 |
| Vancouver | CIVELEK E,CIVELEK D Investigation the role of nitric oxide pathway in TNF-α induced HUVEC cells. Journal of research in pharmacy (online). 2023; 27(4): 1560 - 1566. 10.29228/jrp.441 |
| IEEE | CIVELEK E,CIVELEK D "Investigation the role of nitric oxide pathway in TNF-α induced HUVEC cells." Journal of research in pharmacy (online), 27, ss.1560 - 1566, 2023. 10.29228/jrp.441 |
| ISNAD | CIVELEK, Erkan - CIVELEK, Dilek OZTURK. "Investigation the role of nitric oxide pathway in TNF-α induced HUVEC cells". Journal of research in pharmacy (online) 27/4 (2023), 1560-1566. https://doi.org/10.29228/jrp.441 |
