EXPRESSION LEVELS OF ACE2 AND TMPRSS2 IN DIFFERENT CELL LINES
Yıl: 2023 Cilt: 6 Sayı: 2 Sayfa Aralığı: 260 - 268 Metin Dili: İngilizce DOI: 10.53446/actamednicomedia.1253701 İndeks Tarihi: 30-07-2023
EXPRESSION LEVELS OF ACE2 AND TMPRSS2 IN DIFFERENT CELL LINES
Öz: Objective: ACE2 and TMPRSS2 proteins have received increased attention with the outbreak of pandemic COVID-19. These proteins have roles in respiratory and hypertension disorders as well as cardiovascular and renal diseases. The objective of this work was to examine the mRNA and protein levels of ACE2 and TMPRSS2 in cell lines derived from various tissue origins. Methods: After the growth of 14 different cell lines, protein and mRNA were isolated from the cell pellets. The amounts of mRNAs and proteins were then determined and quantified using qRT-PCR and ELISA. Results: Findings showed that VERO, HEK293T, and VERO E6 cell lines significantly differed from others in the mRNA levels of both the ACE2 and TMPRSS2 genes. In protein levels obtained using ELISA, PNT1A cell line had the highest level of ACE2 protein expression, while for TMPRSS2, A549 had the highest level of protein expression. Conclusions: It has found in this study how the expressions levels of ACE2 and TMPRSS2 depend on the cell type. This may be an explanation for why virulence entrance differs in different types of tissues. It is speculated that HEK293T cells with high levels of both genes may be a suitable option for studies at the RNA level by using these two genes. MCF7 may be a good candidate for studies at the protein level. Given the high levels of mRNA expression of both genes, it may be inferred that cells derived from the kidney were among those that were most susceptible to virus entry.
Anahtar Kelime: ACE2 VE TMPRSS2 GENLERİNİN FARKLI HÜCRE HATLARINDAKİ İFADE DÜZEYLERİ
Öz: Amaç: ACE2 ve TMPRSS2 proteinleri, COVID-19 pandemisi ile birlikte önem kazanmıştır. Bu proteinlerin solunum ve hipertansiyon bozukluklarının yanı sıra kardiyovasküler ve renal hastalıklarda rolleri vardır. Bu çalışmanın amacı, çeşitli dokulardan üretilen hücre hatlarında ACE2 ve TMPRSS2'nin mRNA ve protein seviyelerini belirlemektir. Yöntem: 14 hücre hattı kültürde çoğaltıldıktan sonra, hücre pelletlerinden protein ve mRNA izole edildi. Ardından mRNA seviyeleri qRT-PCR ve protein seviyeleri ise ELISA metotları kullanılarak ölçüldü. Bulgular: Çalışmanın sonuçları, ACE2 ve TMPRSS2 genlerinin mRNA seviyelerinin VERO, HEK293T ve VERO E6 hücre hatlarında, diğer hücre hatlarına kıyasla, önemli ölçüde yüksek olduğunu göstermiştir. ELISA kullanılarak elde edilen protein seviyelerinde, PNT1A hücre hattı en yüksek ACE2 protein ifadesine sahipken, TMPRSS2 için A549 en yüksek protein ekspresyonuna sahip olarak bulunmuştur. Sonuç: Bu çalışmada ACE2 ve TMPRSS2 gen ifadelerinin hücre tipine göre farklılaşabileceği gösterilmiştir. Bu sonuç, farklı doku tiplerinde virüs girişinin neden farklı olduğunun bir açıklaması olabilir. Her iki genin de yüksek düzeyde bulunduğu HEK293T hücrelerinin, bu iki gen kullanılarak RNA düzeyinde yapılacak çalışmalar için uygun bir seçenek olabileceği düşünülmektedir. MCF7 hücre hattı ise protein seviyesindeki çalışmalar için iyi bir seçenek olabilir. Her iki genin yüksek mRNA ekspresyon seviyeleri göz önüne alındığında, böbrekten üretilen hücre hatlarının virüs girişine en duyarlı olanlar arasında olduğu sonucuna varılabilir.
Anahtar Kelime: Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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| APA | Bal Albayrak M, Yanar S, Kasap M, Akpınar G (2023). EXPRESSION LEVELS OF ACE2 AND TMPRSS2 IN DIFFERENT CELL LINES. , 260 - 268. 10.53446/actamednicomedia.1253701 |
| Chicago | Bal Albayrak Merve Gülsen,Yanar Sevinc,Kasap Murat,Akpınar Gürler EXPRESSION LEVELS OF ACE2 AND TMPRSS2 IN DIFFERENT CELL LINES. (2023): 260 - 268. 10.53446/actamednicomedia.1253701 |
| MLA | Bal Albayrak Merve Gülsen,Yanar Sevinc,Kasap Murat,Akpınar Gürler EXPRESSION LEVELS OF ACE2 AND TMPRSS2 IN DIFFERENT CELL LINES. , 2023, ss.260 - 268. 10.53446/actamednicomedia.1253701 |
| AMA | Bal Albayrak M,Yanar S,Kasap M,Akpınar G EXPRESSION LEVELS OF ACE2 AND TMPRSS2 IN DIFFERENT CELL LINES. . 2023; 260 - 268. 10.53446/actamednicomedia.1253701 |
| Vancouver | Bal Albayrak M,Yanar S,Kasap M,Akpınar G EXPRESSION LEVELS OF ACE2 AND TMPRSS2 IN DIFFERENT CELL LINES. . 2023; 260 - 268. 10.53446/actamednicomedia.1253701 |
| IEEE | Bal Albayrak M,Yanar S,Kasap M,Akpınar G "EXPRESSION LEVELS OF ACE2 AND TMPRSS2 IN DIFFERENT CELL LINES." , ss.260 - 268, 2023. 10.53446/actamednicomedia.1253701 |
| ISNAD | Bal Albayrak, Merve Gülsen vd. "EXPRESSION LEVELS OF ACE2 AND TMPRSS2 IN DIFFERENT CELL LINES". (2023), 260-268. https://doi.org/10.53446/actamednicomedia.1253701 |
| APA | Bal Albayrak M, Yanar S, Kasap M, Akpınar G (2023). EXPRESSION LEVELS OF ACE2 AND TMPRSS2 IN DIFFERENT CELL LINES. Acta Medica Nicomedia, 6(2), 260 - 268. 10.53446/actamednicomedia.1253701 |
| Chicago | Bal Albayrak Merve Gülsen,Yanar Sevinc,Kasap Murat,Akpınar Gürler EXPRESSION LEVELS OF ACE2 AND TMPRSS2 IN DIFFERENT CELL LINES. Acta Medica Nicomedia 6, no.2 (2023): 260 - 268. 10.53446/actamednicomedia.1253701 |
| MLA | Bal Albayrak Merve Gülsen,Yanar Sevinc,Kasap Murat,Akpınar Gürler EXPRESSION LEVELS OF ACE2 AND TMPRSS2 IN DIFFERENT CELL LINES. Acta Medica Nicomedia, vol.6, no.2, 2023, ss.260 - 268. 10.53446/actamednicomedia.1253701 |
| AMA | Bal Albayrak M,Yanar S,Kasap M,Akpınar G EXPRESSION LEVELS OF ACE2 AND TMPRSS2 IN DIFFERENT CELL LINES. Acta Medica Nicomedia. 2023; 6(2): 260 - 268. 10.53446/actamednicomedia.1253701 |
| Vancouver | Bal Albayrak M,Yanar S,Kasap M,Akpınar G EXPRESSION LEVELS OF ACE2 AND TMPRSS2 IN DIFFERENT CELL LINES. Acta Medica Nicomedia. 2023; 6(2): 260 - 268. 10.53446/actamednicomedia.1253701 |
| IEEE | Bal Albayrak M,Yanar S,Kasap M,Akpınar G "EXPRESSION LEVELS OF ACE2 AND TMPRSS2 IN DIFFERENT CELL LINES." Acta Medica Nicomedia, 6, ss.260 - 268, 2023. 10.53446/actamednicomedia.1253701 |
| ISNAD | Bal Albayrak, Merve Gülsen vd. "EXPRESSION LEVELS OF ACE2 AND TMPRSS2 IN DIFFERENT CELL LINES". Acta Medica Nicomedia 6/2 (2023), 260-268. https://doi.org/10.53446/actamednicomedia.1253701 |
