Objective: Remineralization technologies have been shown to arrest or reverse early carious lesions. This study aimed to evaluate and compare the effect of different agents on enamel remineralization in-vitro. Methods: Thirty-five enamel blocks were prepared and divided into the following groups: Group 1; CaGp and Xylitol-containing gel (R.O.C.S Medical Mineral Gel), Group 2; 1100 ppm NaF and 10% CPP-ACP toothpaste (MI Paste One), Group 3; 0.45% SnF2-1150 ppm F toothpaste (Enamelon), Group 4; Positive Control-1450 ppm NaF toothpaste (Colgate Total) and Group 5; Negative Control (deionized water). Microhardness was measured at baseline, after demineralization, and after respective treatments for different treatment groups using a digital Micro Vickers Hardness Tester. For producing demineralized lesions, samples were stored in acidic hydroxyethylcellulose (HEC, pH=4.8) for three days. The Paired Sample t-test, one-way ANOVA, and Tukey were used to compare data and SMH recovery (%SMHR) calculated among treatments. Results: The mean baseline surface microhardness value was statistically non-significant between the groups (p=.378). CaGP and Xylitolcontaining gel demonstrated having the most protective effect against demineralization. The surface remineralization potential of 1100 ppm NaF and 10% CPP-ACP containing novel toothpaste (MI Paste One) was almost similar to the positive control (1450 ppm Fluoride toothpaste)group (p>.05). Conclusions: All treatment groups showed remineralization after respective treatments and these agents can be used as an effective preventive measure for pediatric patients.