Expression of dual-specificity phosphatases in TGFβ1-induced EMT in SKOV3 cells

Sabire GÜLER, (Bursa Uludağ Üniversitesi, Veteriner Fakültesi, Histoloji ve Embriyoloji Anabilim Dalı, Bursa, Türkiye)
Abdullah YALÇIN (Bursa Uludağ Üniversitesi, Veteriner Fakültesi, Biyokimya Anabilim Dalı, Bursa, Türkiye)
Turkish Journal of Medical Sciences
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Yıl: 2023 Cilt: 53 Sayı: 3 Sayfa Aralığı: 640 - 646 Metin Dili: İngilizce DOI: 10.55730/1300-0144.5626 İndeks Tarihi: 01-08-2023

Expression of dual-specificity phosphatases in TGFβ1-induced EMT in SKOV3 cells

Öz:
Background/aim: The study aims to profile the dual-specificity phosphatases (DUSP) expression in response to Transforming growth factor β1 (TGFβ1)-induced epithelial- mesenchymal transition (EMT) in ovarian adenocarcinoma cells. Materials and methods: The ovarian adenocarcinoma cell line SKOV3 was used as a TGFβ1-induced EMT model. Cells were incubated with 5 ng/mL TGFβ1 to induce EMT. EMT was confirmed with real-time qPCR, western blot, and immunofluorescence analyses of various EMT markers. Western blot was used to analyze phospho- and total MAPK protein levels. Typical and atypical DUSPs mRNA expression profile was determined by real-time qPCR. Results: The epithelial marker E-cadherin expressions were decreased and mesenchymal EMT markers Snail and Slug expression levels were increased after TGFβ1 induction. Phosphorylation of ERK1/2 and p38 MAPK were enhanced in response to TGFβ1 treatment. The expression of DUSP2, DUSP6, DUSP8, DUSP10, and DUSP13 were decreased while DUSP7, DUSP16, DUSP18, DUSP21, and DUSP27 were increased by TGFβ1. Conclusion: TGFβ1 induced EMT which was accompanied by increased activity of MAPKs, and led to marked changes in expressions of several DUSPs in SKOV3 cells.
Anahtar Kelime: Dual-specificity phosphatases epithelial-mesenchymal transition TGFβ1 ovarian carcinoma SKOV3 MAPK

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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APA guler s, Yalcin A (2023). Expression of dual-specificity phosphatases in TGFβ1-induced EMT in SKOV3 cells. , 640 - 646. 10.55730/1300-0144.5626
Chicago guler sabire,Yalcin Abdullah Expression of dual-specificity phosphatases in TGFβ1-induced EMT in SKOV3 cells. (2023): 640 - 646. 10.55730/1300-0144.5626
MLA guler sabire,Yalcin Abdullah Expression of dual-specificity phosphatases in TGFβ1-induced EMT in SKOV3 cells. , 2023, ss.640 - 646. 10.55730/1300-0144.5626
AMA guler s,Yalcin A Expression of dual-specificity phosphatases in TGFβ1-induced EMT in SKOV3 cells. . 2023; 640 - 646. 10.55730/1300-0144.5626
Vancouver guler s,Yalcin A Expression of dual-specificity phosphatases in TGFβ1-induced EMT in SKOV3 cells. . 2023; 640 - 646. 10.55730/1300-0144.5626
IEEE guler s,Yalcin A "Expression of dual-specificity phosphatases in TGFβ1-induced EMT in SKOV3 cells." , ss.640 - 646, 2023. 10.55730/1300-0144.5626
ISNAD guler, sabire - Yalcin, Abdullah. "Expression of dual-specificity phosphatases in TGFβ1-induced EMT in SKOV3 cells". (2023), 640-646. https://doi.org/10.55730/1300-0144.5626
APA guler s, Yalcin A (2023). Expression of dual-specificity phosphatases in TGFβ1-induced EMT in SKOV3 cells. Turkish Journal of Medical Sciences, 53(3), 640 - 646. 10.55730/1300-0144.5626
Chicago guler sabire,Yalcin Abdullah Expression of dual-specificity phosphatases in TGFβ1-induced EMT in SKOV3 cells. Turkish Journal of Medical Sciences 53, no.3 (2023): 640 - 646. 10.55730/1300-0144.5626
MLA guler sabire,Yalcin Abdullah Expression of dual-specificity phosphatases in TGFβ1-induced EMT in SKOV3 cells. Turkish Journal of Medical Sciences, vol.53, no.3, 2023, ss.640 - 646. 10.55730/1300-0144.5626
AMA guler s,Yalcin A Expression of dual-specificity phosphatases in TGFβ1-induced EMT in SKOV3 cells. Turkish Journal of Medical Sciences. 2023; 53(3): 640 - 646. 10.55730/1300-0144.5626
Vancouver guler s,Yalcin A Expression of dual-specificity phosphatases in TGFβ1-induced EMT in SKOV3 cells. Turkish Journal of Medical Sciences. 2023; 53(3): 640 - 646. 10.55730/1300-0144.5626
IEEE guler s,Yalcin A "Expression of dual-specificity phosphatases in TGFβ1-induced EMT in SKOV3 cells." Turkish Journal of Medical Sciences, 53, ss.640 - 646, 2023. 10.55730/1300-0144.5626
ISNAD guler, sabire - Yalcin, Abdullah. "Expression of dual-specificity phosphatases in TGFβ1-induced EMT in SKOV3 cells". Turkish Journal of Medical Sciences 53/3 (2023), 640-646. https://doi.org/10.55730/1300-0144.5626
Sayfa Oluşturulma Tarihi
24-04-2024 8:43

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