Anti-oxidant, Anti-inflammatory, and Anti-apoptotic Effects of Rutin in Spleen Toxicity Induced by Sodium Valproate in Rats

Nurhan AKARAS, (Aksaray Üniversitesi, Tıp Fakültesi, Histoloji ve Embriyoloji Anabilim Dalı, Aksaray, Türkiye)
Fatih Mehmet KANDEMİR, (Aksaray Üniversitesi, Tıp Fakültesi, Tıbbi Biyokimya Anabilim Dalı, Aksaray, Türkiye)
Hasan Simsek, (Aksaray Üniversitesi, Tıp Fakültesi, Fizyoloji Anabilim Dalı, Aksaray, Türkiye)
Cihan GÜR, (Atatürk Üniversitesi, Veteriner Fakültesi, Veteriner Biyokimya Anabilim Dalı, Erzurum, Türkiye)
Serpil AYGÖRMEZ (Kafkas Üniversitesi, Veteriner Fakültesi, Veteriner Biyokimyası Anabilim Dalı, Kars, Türkiye)
Türk Doğa ve Fen Dergisi
6 2
Yıl: 2023 Cilt: 12 Sayı: 2 Sayfa Aralığı: 138 - 144 Metin Dili: İngilizce DOI: 10.46810/tdfd.1299663 İndeks Tarihi: 08-08-2023

Anti-oxidant, Anti-inflammatory, and Anti-apoptotic Effects of Rutin in Spleen Toxicity Induced by Sodium Valproate in Rats

Öz:
Long-term exposure to sodium valproate, an anti-epileptic drug, causes toxic effects in tissues, especially by increasing oxidative stress and inflammation. Rutin is a flavanoid with anti- oxidant, anti-inflammatory and anti-apoptotic effects found naturally in many plants. In this study, we aimed to investigate the effects of rutin, a natural anti-oxidant, on sodium valproate- induced spleen tissue damage. 35 male rats were divided into 5 groups as control, sodium valproate, rutin, sodium valproate+Rutin 50 and sodium valproate+Rutin 100 groups. For 14 days, 500 mg/kg dose of sodium valproate and 50 or 100 mg/kg of rutin were administered by oral gavage. On day 15, spleen tissues were removed and biochemical methods, oxidative stress, inflammation and apoptotic parameters were analyzed and histologic analysis was performed. The levels of sodium valproate-induced oxidative stress, inflammation and apoptosis parameters increased in spleen tissues compared to the control group (p<0.05). With routine administration, all of these sodium valproate-induced increases were decreased (p<0.05). It was concluded that rutin has potential protective properties against the toxic effect caused by sodium valproate exposure in spleen tissues.
Anahtar Kelime: Sodium Valproate Rutin Oxidative stress Inflammation Apoptosis Spleen Toxicity

Sıçanlarda Sodyum Valproat ile İndüklenen Dalak Toksisitesinde Rutinin Anti-oksidan, Anti-inflamatuvar ve Anti-apoptotik Etkileri

Öz:
Anti-epileptik bir ilaç olan sodyum valproatın uzun süreli maruziyeti, dokularda özellikle oksidatif stres ve inflamasyon artışına neden olarak toksik etki oluşturur. Rutin, birçok bitkide doğal olarak bulunan anti-oksidan, anti-inflamatuvar ve anti-apoptotik etkilere sahip bir flavanoiddir. Bu çalışmada, sodyum valproat kaynaklı dalak doku hasarı üzerine doğal bir anti- oksidan olan rutinin etkilerinin araştırılması amaçlanmıştır. 35 adet erkek sıçan kontrol, sodyum valproat, rutin, sodyum valproat+Rutin 50 ve sodyum valproat+Rutin 100 grupları olmak üzere 5 gruba ayrıldı. 14 gün boyunca 500 mg/kg dozda sodyum valproat uygulamasıyla birlikte 50 veya 100 mg/kg rutin uygulaması oral gavaj yolla yapıldı. 15. günde dalak dokuları alındı ve biyokimyasal yöntemler ile oksidatif stres, inflamasyon ve apoptotik parametrelerin analizi ve histolojik analizler yapıldı. Dalak dokularında sodyum valproat kaynaklı oksidatif stres, inflamasyon ve apoptoz parametrelerin düzeyleri kontrol grubuna göre artmıştır (p<0,05). Rutin uygulamasıyla birlikte sodyum valproata bağlı tüm bu artış miktarlarında azalmalar meydana gelmiştir (p<0,05). Dalak dokularında sodyum valproat maruziyetinin neden olduğu toksik etkiye karşı rutinin potansiyel koruyucu özelliklere sahip olduğu sonucuna varıldı.
Anahtar Kelime: Sodyum Valproat Rutin Oksidatif stres İnflamasyon Apoptoz Dalak Toksisitesi

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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APA akaras n, KANDEMİR F, Simsek H, GÜR C, AYGÖRMEZ S (2023). Anti-oxidant, Anti-inflammatory, and Anti-apoptotic Effects of Rutin in Spleen Toxicity Induced by Sodium Valproate in Rats. , 138 - 144. 10.46810/tdfd.1299663
Chicago akaras nurhan,KANDEMİR Fatih Mehmet,Simsek Hasan,GÜR Cihan,AYGÖRMEZ Serpil Anti-oxidant, Anti-inflammatory, and Anti-apoptotic Effects of Rutin in Spleen Toxicity Induced by Sodium Valproate in Rats. (2023): 138 - 144. 10.46810/tdfd.1299663
MLA akaras nurhan,KANDEMİR Fatih Mehmet,Simsek Hasan,GÜR Cihan,AYGÖRMEZ Serpil Anti-oxidant, Anti-inflammatory, and Anti-apoptotic Effects of Rutin in Spleen Toxicity Induced by Sodium Valproate in Rats. , 2023, ss.138 - 144. 10.46810/tdfd.1299663
AMA akaras n,KANDEMİR F,Simsek H,GÜR C,AYGÖRMEZ S Anti-oxidant, Anti-inflammatory, and Anti-apoptotic Effects of Rutin in Spleen Toxicity Induced by Sodium Valproate in Rats. . 2023; 138 - 144. 10.46810/tdfd.1299663
Vancouver akaras n,KANDEMİR F,Simsek H,GÜR C,AYGÖRMEZ S Anti-oxidant, Anti-inflammatory, and Anti-apoptotic Effects of Rutin in Spleen Toxicity Induced by Sodium Valproate in Rats. . 2023; 138 - 144. 10.46810/tdfd.1299663
IEEE akaras n,KANDEMİR F,Simsek H,GÜR C,AYGÖRMEZ S "Anti-oxidant, Anti-inflammatory, and Anti-apoptotic Effects of Rutin in Spleen Toxicity Induced by Sodium Valproate in Rats." , ss.138 - 144, 2023. 10.46810/tdfd.1299663
ISNAD akaras, nurhan vd. "Anti-oxidant, Anti-inflammatory, and Anti-apoptotic Effects of Rutin in Spleen Toxicity Induced by Sodium Valproate in Rats". (2023), 138-144. https://doi.org/10.46810/tdfd.1299663
APA akaras n, KANDEMİR F, Simsek H, GÜR C, AYGÖRMEZ S (2023). Anti-oxidant, Anti-inflammatory, and Anti-apoptotic Effects of Rutin in Spleen Toxicity Induced by Sodium Valproate in Rats. Türk Doğa ve Fen Dergisi, 12(2), 138 - 144. 10.46810/tdfd.1299663
Chicago akaras nurhan,KANDEMİR Fatih Mehmet,Simsek Hasan,GÜR Cihan,AYGÖRMEZ Serpil Anti-oxidant, Anti-inflammatory, and Anti-apoptotic Effects of Rutin in Spleen Toxicity Induced by Sodium Valproate in Rats. Türk Doğa ve Fen Dergisi 12, no.2 (2023): 138 - 144. 10.46810/tdfd.1299663
MLA akaras nurhan,KANDEMİR Fatih Mehmet,Simsek Hasan,GÜR Cihan,AYGÖRMEZ Serpil Anti-oxidant, Anti-inflammatory, and Anti-apoptotic Effects of Rutin in Spleen Toxicity Induced by Sodium Valproate in Rats. Türk Doğa ve Fen Dergisi, vol.12, no.2, 2023, ss.138 - 144. 10.46810/tdfd.1299663
AMA akaras n,KANDEMİR F,Simsek H,GÜR C,AYGÖRMEZ S Anti-oxidant, Anti-inflammatory, and Anti-apoptotic Effects of Rutin in Spleen Toxicity Induced by Sodium Valproate in Rats. Türk Doğa ve Fen Dergisi. 2023; 12(2): 138 - 144. 10.46810/tdfd.1299663
Vancouver akaras n,KANDEMİR F,Simsek H,GÜR C,AYGÖRMEZ S Anti-oxidant, Anti-inflammatory, and Anti-apoptotic Effects of Rutin in Spleen Toxicity Induced by Sodium Valproate in Rats. Türk Doğa ve Fen Dergisi. 2023; 12(2): 138 - 144. 10.46810/tdfd.1299663
IEEE akaras n,KANDEMİR F,Simsek H,GÜR C,AYGÖRMEZ S "Anti-oxidant, Anti-inflammatory, and Anti-apoptotic Effects of Rutin in Spleen Toxicity Induced by Sodium Valproate in Rats." Türk Doğa ve Fen Dergisi, 12, ss.138 - 144, 2023. 10.46810/tdfd.1299663
ISNAD akaras, nurhan vd. "Anti-oxidant, Anti-inflammatory, and Anti-apoptotic Effects of Rutin in Spleen Toxicity Induced by Sodium Valproate in Rats". Türk Doğa ve Fen Dergisi 12/2 (2023), 138-144. https://doi.org/10.46810/tdfd.1299663
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