Yıl: 2023 Cilt: 47 Sayı: 4 Sayfa Aralığı: 334 - 344 Metin Dili: İngilizce DOI: 10.55730/1300-0128.4302 İndeks Tarihi: 01-09-2023

Stereological investigation of medulla oblongata volume in Norduz sheep

Öz:
In this study, it was aimed to stereologically examine the medulla oblongata volume of Norduz sheep in the Gürpınar District of Van Province. Upon receiving news from the sections in the Gürpınar region, we went to the region. A total of 12 Norduz sheep skulls, 6 females and 6 males, were received from the Norduz sheep slaughter in the region. After the brains of Norduz sheep were dissected, they were fixed as a whole in formaldehyde for 1 week. Medulla oblongatas were separated from the brain and kept in formaldehyde for 1 week. Tissues obtained by sequential random sampling from the medulla oblongata were used for texture tracking. After texture tracking, 10–12 sections of 5 μm thickness were taken by sequential random sampling method using a rotary microtome (Leica RM, 2135, Leica Instruments, Nussloch, Germany) using steel disposable microtome blades. These obtained sections were stained with the hematoxylin-eosin staining method and viewed under an Olympus CX31 microscope with Olympus U - TV0, 5XC - 3 Tokyo Japan. Photos taken with a 4× objective were used for volume calculations. Total medulla oblongata volume, medulla volume, and cortex volume values were calculated separately from the obtained images using the Shtereom I program. The Cavalieri’s principle was used when applying stereological methods for volume calculations. In the statistical evaluation between the sexes, no difference was found in the values obtained in the comparison between the total volume values, medulla volume values, and cortex volume values between both sexes. In addition, there was no difference between the sexes in terms of medulla/total volume ratio values. However, a value of 0.004 was obtained in terms of cortex/total volume ratio values. In this case, since p < 0.005, it is possible to talk about a statistically significant difference in male and female sheep in terms of this ratio value. Again, the medulla/cortex volume ratio value was calculated to be 0.041, and a significant difference was observed in terms of this value in the evaluation between sexes. The volume calculations obtained by stereological methods were evaluated with statistical programs and all the results were presented to the literature.
Anahtar Kelime: Cavalieri’s principle medulla oblongata Norduz sheep stereology volume

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
  • 1. Bingol M. Reproductive and milk yield with growth- development and morphological traits of Norduz Sheep. PhD, University of Yuzuncu Yil, Van, Türkiye, 1998.
  • 2. Gökdal Ö. Karakaş koyunlarının süt ve döl verimleri ile dış yapı ve büyüme gelişme özellikleri üzerine araştırmalar. PhD, Yüzüncü Yıl Üniversitesi, Van, Türkiye, 1998 (in Turkish).
  • 3. Dayan Y, Bingöl M. Norduz koyunu yetiştiriciliği yapılan kimi işletmelerin yapısal özellikleri. Yüzüncü Yıl Üniversitesi Fen Bilimleri Dergisi 2008; 13 (1): 31-34 (in Turkish).
  • 4. Dursun N. Veteriner Anatomi III. Ankara, Türkiye: MedisanYayınevi; 2008 (in Turkish).
  • 5. Dyce KM, Sack WO, Wensing CJK. Veteriner Anatomi Konu Anlatımı ve Atlas. Hazıroğlu M, Çakır A, (Translators) 4th ed. Ankara, Türkiye: Güneş Tıp Kitabevleri; 2017 (in Turkish).
  • 6. Taner D, Sancak B, Akşit D, Cumhur M, İlgi S et al. Fonksiyonel Nöroanatomi. In: Taner D, (editor) 6th ed. Ankara, Türkiye: ODTÜ Yayınevi; 2007. pp. 49-88 (in Turkish).
  • 7. Getty R. Sissons and Grossmann’s the anatomy of the domestic animals. 5th ed. Phiadelphia, USA: W B Saunders Company; 1975. pp. 182-226.
  • 8. Tecirlioğlu S. Komparatif Veteriner Anatomi Sinir Sistemi. 1st ed. Ankara, Türkiye: Ankara Üniversitesi Veteriner Fakültesi Yayınları; 1983. pp. 1-179 (in Turkish).
  • 9. Türkmenoğlu İ, Demirkan AÇ, Özdemir V, Akosman MS. Veteriner Anatomi Evcil Memeli Hayvanlar. In: Kürtül İ, Türkmenoğlu İ, (editors). Sinir sistemi (systema nervosum). Malatya, Türkiye: Medipres Matbaacılık Yayıncılık; 2015. pp. 495- 469 (in Turkish).
  • 10. Mayhew TM, Gundersen HJG. If you assume, you can make an ass out of u and me: A decade of the disector for stereological counting of particles in 3D space. Journal of Anatomy 1996; 188 (1): 1-15.
  • 11. Mouton PR. Principles and practices unbiased stereology. Baltimore, USA: The Johns Hopkins University Press; 2002.
  • 12. Cruz-Orive LM, Weibel ER. Recent stereological methods for cell biology: a brief survey. American Journal of Physiology-Lung Cellular and Molecular Physiology 1990; 258 (4): 148-156. https:// doi.org/10.1152/ajplung.1990.258.4.L148
  • 13. Gundersen HJG. Stereology of arbitrary particles: A review of unbiased number and size estimators and the presentation of some new ones in memory of William R Thomson. Journal of Microscopy 1986; 143 (1): 3-45.
  • 14. Odacı E, Yıldırım Ş, Bahadır A, Canan S, Şahin B et al. The possible error sources of new stereological methods and their solutions. Turkiye Klinikleri Journal of Medical Sciences 2004; 24 (1): 78-87.
  • 15. Howard CV, Reed MG. Unbiased stereology. Three-dimensional measurement in microscopy. Oxford, UK: Bios Scientific Publishers; 1998; pp. 39-54.
  • 16. Howard CV, Reed MG. Unbiased Stereology. Three-dimensional measurement in microscopy. 2nd ed. Oxford, UK: Bios Scientific Publishers; 2005.
  • 17. Oğuz OE, Çonkur ŞE, Sarı M. Shtereom I simple wındows based software for stereology volume and number estimations. Image Analysis & Stereology 2007; 26 (1): 45-50. https://doi. org/10.5566/ias.v26.p45-50
  • 18. GundersenHJG,JensenEB.Theefficiencyofsystematicsampling in stereology and its prediction. Journal of Microscopy 1987; 147 (3): 229-263. https://doi.org/10.1111/j.1365-2818.1987. tb02837.x
  • 19. Gundersen HJG, Jensen EBV, Kieu K, Nielsen J. The efficiency of systematic sampling in stereology-reconsidered. Journal of Microscopy 1999; 193 (3): 199-211. https://doi.org/10.1046/ j.1365-2818.1999.00457.x
  • 20. Garcia-Finana M, Cruz-Orive LM, Mackay CE, Pakkenberg B, Roberts N. Comparison of MR imaging against physical sectioning to estimate the volume of human cerebral compartments. Neuroimage. 2003; 18 (2): 505-516. https://doi. org/10.1016/S1053-8119(02)00021-6
  • 21. Aydoğdu S, Eken E. Koyun beyninin 3 tesla manyetik rezonans görüntülerinin üç boyutlu rekonstrüksiyonu. PhD, Selçuk Üniversitesi, Konya, Türkiye, 2021.
  • 22. Bakici C, Ekim O, Ergin I, Algın O. Three-dimensional reconstruction from 3 tesla magnetic resonance images of sheep brain. Journal of Ankara University Faculty of Veterinary Medicine 2019; 67 (1): 11-14. https://doi.org/10.33988/ auvfd.548939
  • 23. Aydoğdu S. Akkaraman koyunu ve kıl keçisi beyin hemisferlerine ait substantia grisea, alba ve ventriculus lateralis hacimlerinin Cavalieri prensibi kullanılarak hesaplanması. PhD, Selçuk Üniversitesi, Konya, Türkiye, 2016 (in Turkish).
  • 24. Sadeghinezhad J, Zadsar N, Hasanzadeh B. Morphometric changes in the spinal cord during prenatal life: a stereological study in sheep. Anatomical Science International 2018; 93: 269- 276. https://doi.org/10.1007/s12565-017-0407-9
  • 25. Sterio DC. The unbiased estimation of number and sizes of arbitraryparticlesusingthedisector.JournalofMicroscopy 1984; 134 (2): 127-136. https://doi.org/10.1111/j.1365-2818.1984. tb02501.x
  • 26. Akalan MA, Çevik Demirkan A. Stereoloji ve veteriner hekimlikte kullanım alanları. YYÜ Veteriner Fakültesi Dergisi 2013; 24 (2): 95-100 (in Turkish).
  • 27. Yurt KK, Kivrak EG, Altun G, Mohamed H, Ali F et al. A brief update on physical and optical disector applications and sectioning-staining methods in neuroscience. Journal of Chemical Neuroanatomy 2018; 93: 16-29. https://doi. org/10.1016/j.jchemneu.2018.02.009
APA ERTAŞ O, Çakmak g (2023). Stereological investigation of medulla oblongata volume in Norduz sheep. , 334 - 344. 10.55730/1300-0128.4302
Chicago ERTAŞ OZAN,Çakmak gamze Stereological investigation of medulla oblongata volume in Norduz sheep. (2023): 334 - 344. 10.55730/1300-0128.4302
MLA ERTAŞ OZAN,Çakmak gamze Stereological investigation of medulla oblongata volume in Norduz sheep. , 2023, ss.334 - 344. 10.55730/1300-0128.4302
AMA ERTAŞ O,Çakmak g Stereological investigation of medulla oblongata volume in Norduz sheep. . 2023; 334 - 344. 10.55730/1300-0128.4302
Vancouver ERTAŞ O,Çakmak g Stereological investigation of medulla oblongata volume in Norduz sheep. . 2023; 334 - 344. 10.55730/1300-0128.4302
IEEE ERTAŞ O,Çakmak g "Stereological investigation of medulla oblongata volume in Norduz sheep." , ss.334 - 344, 2023. 10.55730/1300-0128.4302
ISNAD ERTAŞ, OZAN - Çakmak, gamze. "Stereological investigation of medulla oblongata volume in Norduz sheep". (2023), 334-344. https://doi.org/10.55730/1300-0128.4302
APA ERTAŞ O, Çakmak g (2023). Stereological investigation of medulla oblongata volume in Norduz sheep. Turkish Journal of Veterinary and Animal Sciences, 47(4), 334 - 344. 10.55730/1300-0128.4302
Chicago ERTAŞ OZAN,Çakmak gamze Stereological investigation of medulla oblongata volume in Norduz sheep. Turkish Journal of Veterinary and Animal Sciences 47, no.4 (2023): 334 - 344. 10.55730/1300-0128.4302
MLA ERTAŞ OZAN,Çakmak gamze Stereological investigation of medulla oblongata volume in Norduz sheep. Turkish Journal of Veterinary and Animal Sciences, vol.47, no.4, 2023, ss.334 - 344. 10.55730/1300-0128.4302
AMA ERTAŞ O,Çakmak g Stereological investigation of medulla oblongata volume in Norduz sheep. Turkish Journal of Veterinary and Animal Sciences. 2023; 47(4): 334 - 344. 10.55730/1300-0128.4302
Vancouver ERTAŞ O,Çakmak g Stereological investigation of medulla oblongata volume in Norduz sheep. Turkish Journal of Veterinary and Animal Sciences. 2023; 47(4): 334 - 344. 10.55730/1300-0128.4302
IEEE ERTAŞ O,Çakmak g "Stereological investigation of medulla oblongata volume in Norduz sheep." Turkish Journal of Veterinary and Animal Sciences, 47, ss.334 - 344, 2023. 10.55730/1300-0128.4302
ISNAD ERTAŞ, OZAN - Çakmak, gamze. "Stereological investigation of medulla oblongata volume in Norduz sheep". Turkish Journal of Veterinary and Animal Sciences 47/4 (2023), 334-344. https://doi.org/10.55730/1300-0128.4302