In this study, changes in heart-type fatty acid binding protein (H-FABP) and certain biochemical parameters during chronic artery diseases were investigated. Coronary artery disease (CAD) is one of the leading causes of death and morbidity in our country,which is also true for the world in general as well. CAD generally develops on the foundations of atherosclerosis. In this study, blood samples collected from patients who applied to Van Yüzüncü Yıl University Dursun Odabaş Medical Center Emergency Department and Cardiology Polyclinics with acute ischemic chest pain between January 2019 and June 2019 were evaluated. Patients were not given any drugs or other kinds of substances before sample collection. Of the patients who applied to the cardiology clinic, 24 patients with acute ischemic chest pain were diagnosed with chronic arteritis, 12 patients with cardiological problems were diagnosed with diabetes mellitus (DM), 12 patients with hypertension (HT) problems and heart complaints, and 12 healthy individuals (who were not diagnosed with diabetes, hypertension or CAD) were included as research materials. Certain markers like Troponin, CK, CK-MB, AST, ALT, LDL-Cholesterol, HDL-Cholesterol, LDH, Glucose, and Creatinine in the blood samples were biochemically determined using an autoanalyzer (Abbott ci16200),while H-FABP values were determined using ELISA method. LDL-cholesterol levels were highest in the CAD group, HDL-Cholesterol and LDH levels were high in the CAD+HT group, while CK, CK-MB, cTnI, AST, Glucose, creatinine, and H-FABP levels were found to be high in the DM+CADgroup. Meanwhile, cTnI values were increased in people with HT or DM in addition to CAD, but there was no statistical significance. LDL-cholesterol changes also did not vary significantly between the groups. Intergroup changes in other parameters examined showed the importance of CK and Glucose levels at p≤ 0.001, AST p≤ 0.002, LDH p≤ 0.003, CK-MB p≤ 0.004, HDL-Cholesterol p≤ 0.049, Creatinine p≤ 0.011, and H-FABP p≤ 0.050. In recent years, H-FABP has taken its place in the field of cardiology with increasing importance in the diagnosis of CAD and MI. The findings obtained in this study show that the H-FABP level was increased in all patients examined (except the test group), and we recommend its use as a critical and useful parameter in the field of cardiology.