Yıl: 2023 Cilt: 9 Sayı: 3 Sayfa Aralığı: 317 - 324 Metin Dili: Türkçe DOI: 10.53394/akd.1145360 İndeks Tarihi: 05-09-2023

Geçici Serebral İskemi Modelinde Fokal Hipotermik Tedavi

Öz:
Amaç: İskemik inme tedavisinde reperfüzyon yöntemleri ile iyi sonuçlar elde edilmektedir. Reperfüzyonun faydasının yanında inflamasyon ve beyin ödemini içerecek şekilde istenmeyen etkileri de vardır. Reperfüze olan bölgenin fokal olarak ısısının düşürülmesi istenmeyen etkileri azaltarak nöron hasarını azaltabilir. Bu çalışmada deneysel geçici serebral iskemi modelinde, iskemik dallara soğuk serum fizyolojik enjeksiyonu ile lokal olarak sağlanacak beyin ısısındaki düşüşün infarkt alanına ve klinik sonlanıma etkisini göstermeyi amaçladık. Gereç ve Yöntemler: Çalışmada 28 adet rat kullanılarak intralüminal sütür tekniği ile 90 dakikalık geçici orta serebral arter oklüzyonu yapıldı. Rekanalizasyon sonrası kontrol grubunda işlem sonlandırıldı, diğer gruplarda ise internal karotis arter yoluyla sırasıyla 15ºC, 23ºC ve 37ºC ısısında serum fizyolojik infüzyonu yapıldı. Çalışma sırasında 15ºC salin infüzyonu yapılan grupta erken dönem ölümler görülmesi üzerine çalışmanın bu ayağı sonlandırıldı. Diğer gruplar 24. saat sonunda infarkt hacmi ve nörolojik sonlanım açısından karşılaştırıldı. Bulgular: Salin infüzyonu yapılan iki grupta (23 ve 37 ºC) da kontrol grubuna göre infarkt hacimlerinde küçülme saptandı (p=0,012). Salin infüzyonu yapılan gruplar arasında toplam (kortikal ve subkortikal) infarkt hacmi arasında fark yoktu. Kortikal infarkt hacmi açısından yapılan karşılaştırmada 23ºC grupta kontrole kıyasla anlamlı küçülme gösterildi (p=0,011), 37 ºC grup kontrolle kıyaslandığında anlamlı küçülme görülmedi ve sadece fark eğilimi vardı (p=0,063). Nörolojik skor açısından kıyaslama yapıldığında 23ºC’lik infüzyon yapılan grubun skoru diğer gruplara göre daha iyi bulundu (p=0,010). Sonuç: Reperfüzyon sırasında salin infüzyonu ile infarkt hacminde azalma sağlanmıştır. Bu etki soğuk uygulama ile birlikte daha belirgindir. Ayrıca soğuk uygulama yapılan deneklerin nörolojik skorları daha iyidir.
Anahtar Kelime: Serebral iskemi İnfarkt Hipodermi Reperfüzyon hasarı

Focal Hypothermic Treatment in Temporary Cerebral Ischemia Model

Öz:
Objective: Positive results have been attained in ischemic stroke treatment by reperfusion. Despite the benefits of reperfusion there are also certain side effects consisting of inflammation and brain edema. Decreasing the temperature of the reperfused region locally may diminish the unwanted impact and mitigate the neuron damage. In this study, we aimed to show the effect of the decrease in cerebral temperature, which will be provided locally by the injection of cold saline into the ischemic branches, on the infarct area and clinical outcome in the experimental temporary cerebral ischemia model. Material and Methods: Using the 28 rats, 90-minute temporary middle cerebral artery occlusion has been done by using intraluminal suture technique. Following the recanalization the process was terminated for the control group. In the other groups, serum physiologic infusion via internal carotid artery was done in temperatures of 15ºC, 23ºC and 37ºC consecutively. During the study early deaths were observed in the group which 15ºC saline infusion was done therefore, this pillar of the study was terminated. Other groups were compared in terms of infarct volume and neurolog- ical outcome after 24 hours. Results: In both groups which saline infusion was administered (23 and 37 ºC) shrinkage was identified in infarct volumes compared to the control group (p=0,012). There was no difference in total cortical and subcortical infarct volumes between the groups subject to saline infusion. In terms of cortical infarct volume 23ºC group has shown a meaningful shrinkage compared to the control group (p=0,011) whereas 37-ºC group did not have meaningful shrinkage compared to the control and had only a tendency of difference (p=0,063). In comparison of neurologi- cal score, the score of the group infused with 23ºC saline was better compared to the other groups (p=0,010). Conclusion: During the reperfusion shrinkage in infarct volume was attained by the help of saline infusion and the impact is more evident paired with cold administration. Additionally, the neurological scores of cold administered subjects are better.
Anahtar Kelime: Cerebral ischemia Infarct Hypothermia Reperfusion damage

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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APA Unal A, ONAL M (2023). Geçici Serebral İskemi Modelinde Fokal Hipotermik Tedavi. , 317 - 324. 10.53394/akd.1145360
Chicago Unal Ali,ONAL MEHMET ZULKUF Geçici Serebral İskemi Modelinde Fokal Hipotermik Tedavi. (2023): 317 - 324. 10.53394/akd.1145360
MLA Unal Ali,ONAL MEHMET ZULKUF Geçici Serebral İskemi Modelinde Fokal Hipotermik Tedavi. , 2023, ss.317 - 324. 10.53394/akd.1145360
AMA Unal A,ONAL M Geçici Serebral İskemi Modelinde Fokal Hipotermik Tedavi. . 2023; 317 - 324. 10.53394/akd.1145360
Vancouver Unal A,ONAL M Geçici Serebral İskemi Modelinde Fokal Hipotermik Tedavi. . 2023; 317 - 324. 10.53394/akd.1145360
IEEE Unal A,ONAL M "Geçici Serebral İskemi Modelinde Fokal Hipotermik Tedavi." , ss.317 - 324, 2023. 10.53394/akd.1145360
ISNAD Unal, Ali - ONAL, MEHMET ZULKUF. "Geçici Serebral İskemi Modelinde Fokal Hipotermik Tedavi". (2023), 317-324. https://doi.org/10.53394/akd.1145360
APA Unal A, ONAL M (2023). Geçici Serebral İskemi Modelinde Fokal Hipotermik Tedavi. Akdeniz Tıp Dergisi, 9(3), 317 - 324. 10.53394/akd.1145360
Chicago Unal Ali,ONAL MEHMET ZULKUF Geçici Serebral İskemi Modelinde Fokal Hipotermik Tedavi. Akdeniz Tıp Dergisi 9, no.3 (2023): 317 - 324. 10.53394/akd.1145360
MLA Unal Ali,ONAL MEHMET ZULKUF Geçici Serebral İskemi Modelinde Fokal Hipotermik Tedavi. Akdeniz Tıp Dergisi, vol.9, no.3, 2023, ss.317 - 324. 10.53394/akd.1145360
AMA Unal A,ONAL M Geçici Serebral İskemi Modelinde Fokal Hipotermik Tedavi. Akdeniz Tıp Dergisi. 2023; 9(3): 317 - 324. 10.53394/akd.1145360
Vancouver Unal A,ONAL M Geçici Serebral İskemi Modelinde Fokal Hipotermik Tedavi. Akdeniz Tıp Dergisi. 2023; 9(3): 317 - 324. 10.53394/akd.1145360
IEEE Unal A,ONAL M "Geçici Serebral İskemi Modelinde Fokal Hipotermik Tedavi." Akdeniz Tıp Dergisi, 9, ss.317 - 324, 2023. 10.53394/akd.1145360
ISNAD Unal, Ali - ONAL, MEHMET ZULKUF. "Geçici Serebral İskemi Modelinde Fokal Hipotermik Tedavi". Akdeniz Tıp Dergisi 9/3 (2023), 317-324. https://doi.org/10.53394/akd.1145360