Aim: The aim of this study is to evaluate the effect of Ankaferd Blood Stopper (ABS) on the palatal donor site following free gingival graft (FGG) surgery. Materials and Methods: A total of 12 patients (24 palatal sites) were included in the study. In each patient, the donor sites were randomly divided into two groups. ABS+collagen pads were applied to the test group. Only collagen pads were applied to the control group. Donor sites were evaluated in terms of bleeding, complete epithelialization, sensitivity, pain score, analgesic usage, wound size, Landry, Turnbull, and Howley (LTH) index, and tissue thickness (TT). Results: Intraoperative bleeding was observed in significantly more patients (n=9) in the control group, while it was not observed in any of the patients in the test group (p=0.000). Although higher complete epithelialization (33.3%) was detected in the test group on day 14, there was no significant difference in epithelization between the groups. There was no significant difference in pain score, analgesic usage, wound size, LTH index, or TT between the groups. When the amount of change between the times was analyzed, only the change in TT between the 21st day and the 6th week was found to be statistically significant (p=0.028). Conclusion: In our study, it can be said that the use of ABS following FGG surgery has a positive effect on intraoperative bleeding, shortterm complete epithelialization, and long-term TT, but it cannot provide superiority compared to collagen sponge alone in terms of early wound healing, postoperative pain, and analgesic usage.