The Effect of Berberine on the Prevention and/or Treatment on Cyclophosphamide-Induced Testicular Damage in Rats

altuntaş, hakan; Özdemir, Mahmut; Harmanci, Nusin; yigitaslan, semra; Sahinturk, Varol

doi:10.20515/otd.1180404

The Effect of Berberine on the Prevention and/or Treatment on Cyclophosphamide-Induced Testicular Damage in Rats

Hakan ALTUNTAŞ, (Eskişehir Osmangazi Üniversitesi, Tıp Fakültesi, Farmakoloji Bölümü, Eskişehir, Türkiye)

Mahmut ÖZDEMİR, (Eskişehir Osmangazi Üniversitesi, Tıp Fakültesi, Farmakoloji Bölümü, Eskişehir, Türkiye)

Nuşin HARMANCI, (Eskişehir Osmangazi Üniversitesi, Tıp Fakültesi, Farmakoloji Bölümü, Eskişehir, Türkiye)

Semra YİĞİTASLAN, (Eskişehir Osmangazi Üniversitesi, Tıp Fakültesi, Farmakoloji Bölümü, Eskişehir, Türkiye)

Varol ŞAHİNTÜRK (Eskişehir Osmangazi Üniversitesi, Tıp Fakültesi, Histoloji Anabilim Dalı, Eskişehir, Türkiye)

Osmangazi Tıp Dergisi

7 0

Yıl: 2023 Cilt: 45 Sayı: 2 Sayfa Aralığı: 161 - 171 Metin Dili: İngilizce DOI: 10.20515/otd.1180404 İndeks Tarihi: 06-09-2023

The Effect of Berberine on the Prevention and/or Treatment on Cyclophosphamide-Induced Testicular Damage in Rats

Öz:

The protective and/or therapeutic effect of berberine on cyclophosphamide-induced testicular injury was investigated in rats. A total of 40 male Sprague-Dawley rats were divided into 5 groups (n=8 per group). The control group received saline and CP, BER75, BER150 and BER300 groups received single dose of intraperitoneal 200 mg/kg CP on day 8. Berberine (75 mg/kg, 150 mg/kg and 300 mg/kg) was administered orally for 7 days before and after the CP injection. At the end of the protocol, testes were removed for histological examination, immunohistochemical staining, and biochemical assessments. In our study, CP did not cause a significant change in body weight and testis weight, whereas berberine treatment significantly decreased body weight. Although no significant change was observed in terms of oxidative stress markers and cytokine levels, degenerative changes and apoptotic findings were found in the CP group; which were improved significantly with increasing doses of berberine treatment. In conclusion, we found that treatment with berberine may have anti-degenerative and anti-apoptotic potential rather than altering oxidative stress markers and/or inflammatory pathways in CP-induced testicular damage.

Anahtar Kelime: berberine cyclophosphamide rat testicular injury

Sıçanlarda Berberinin Siklofosfamide Bağli Testiküler Hasar Üzerindeki Önleme Ve / Veya Tedavi Edici Etkisi

Öz:

Bu çalışmada Berberinin sıçanlarda siklofosfamide bağlı testis yaralanması üzerindeki koruyucu ve/veya terapötik etkisi araştırıldı. Toplam 40 erkek Sprague-Dawley sıçan 5 gruba ayrıldı (n = 8). Sekizinci günde tek doz olarak kontrol grubuna intraperitoneal salin, CP, BER75, BER150 ve BER300 gruplarına ise intraperitoneal 200 mg/kg CP uygulandı. Serum fizyolojik, 75 mg/kg, 150 mg/kg ve 300 mg/kg berberin, enjeksiyondan 7 gün önce ve 7 gün sonra oral olarak uygulandı. Protokolün sonunda testisler histolojik inceleme, immünhistokimyasal boyama ve biyokimyasal değerlendirme için çıkarıldı. Çalışmamızda CP vücut ağırlığı ve testis ağırlığında önemli bir değişikliğe neden olmazken, berberin tedavisi vücut ağırlığını anlamlı ölçüde düşürdü. Oksidatif stres belirteçleri ve sitokin düzeyleri açısından anlamlı bir değişiklik görülmemekle birlikte CP grubunda dejeneratif değişiklikler ve apoptotik bulgular saptanmıştır, bu bulgular artan berberin te- davisi dozları ile önemli ölçüde iyileşmiştir. Sonuç olarak berberin ile tedavinin, CP'ye bağlı testis hasarında oksidatif stres belirteçlerini ve/ veya inflamatuvar yolları değiştirmekten çok anti-dejeneratif ve anti-apoptotik potansiyele sahip olabileceğini bulduk.

Anahtar Kelime: Berberin Sıçan Siklofosfamid Testis hasarı

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	altuntaş h, Özdemir M, Harmanci N, yigitaslan s, Sahinturk V (2023). The Effect of Berberine on the Prevention and/or Treatment on Cyclophosphamide-Induced Testicular Damage in Rats. , 161 - 171. 10.20515/otd.1180404
Chicago	altuntaş hakan,Özdemir Mahmut,Harmanci Nusin,yigitaslan semra,Sahinturk Varol The Effect of Berberine on the Prevention and/or Treatment on Cyclophosphamide-Induced Testicular Damage in Rats. (2023): 161 - 171. 10.20515/otd.1180404
MLA	altuntaş hakan,Özdemir Mahmut,Harmanci Nusin,yigitaslan semra,Sahinturk Varol The Effect of Berberine on the Prevention and/or Treatment on Cyclophosphamide-Induced Testicular Damage in Rats. , 2023, ss.161 - 171. 10.20515/otd.1180404
AMA	altuntaş h,Özdemir M,Harmanci N,yigitaslan s,Sahinturk V The Effect of Berberine on the Prevention and/or Treatment on Cyclophosphamide-Induced Testicular Damage in Rats. . 2023; 161 - 171. 10.20515/otd.1180404
Vancouver	altuntaş h,Özdemir M,Harmanci N,yigitaslan s,Sahinturk V The Effect of Berberine on the Prevention and/or Treatment on Cyclophosphamide-Induced Testicular Damage in Rats. . 2023; 161 - 171. 10.20515/otd.1180404
IEEE	altuntaş h,Özdemir M,Harmanci N,yigitaslan s,Sahinturk V "The Effect of Berberine on the Prevention and/or Treatment on Cyclophosphamide-Induced Testicular Damage in Rats." , ss.161 - 171, 2023. 10.20515/otd.1180404
ISNAD	altuntaş, hakan vd. "The Effect of Berberine on the Prevention and/or Treatment on Cyclophosphamide-Induced Testicular Damage in Rats". (2023), 161-171. https://doi.org/10.20515/otd.1180404

APA	altuntaş h, Özdemir M, Harmanci N, yigitaslan s, Sahinturk V (2023). The Effect of Berberine on the Prevention and/or Treatment on Cyclophosphamide-Induced Testicular Damage in Rats. Osmangazi Tıp Dergisi, 45(2), 161 - 171. 10.20515/otd.1180404
Chicago	altuntaş hakan,Özdemir Mahmut,Harmanci Nusin,yigitaslan semra,Sahinturk Varol The Effect of Berberine on the Prevention and/or Treatment on Cyclophosphamide-Induced Testicular Damage in Rats. Osmangazi Tıp Dergisi 45, no.2 (2023): 161 - 171. 10.20515/otd.1180404
MLA	altuntaş hakan,Özdemir Mahmut,Harmanci Nusin,yigitaslan semra,Sahinturk Varol The Effect of Berberine on the Prevention and/or Treatment on Cyclophosphamide-Induced Testicular Damage in Rats. Osmangazi Tıp Dergisi, vol.45, no.2, 2023, ss.161 - 171. 10.20515/otd.1180404
AMA	altuntaş h,Özdemir M,Harmanci N,yigitaslan s,Sahinturk V The Effect of Berberine on the Prevention and/or Treatment on Cyclophosphamide-Induced Testicular Damage in Rats. Osmangazi Tıp Dergisi. 2023; 45(2): 161 - 171. 10.20515/otd.1180404
Vancouver	altuntaş h,Özdemir M,Harmanci N,yigitaslan s,Sahinturk V The Effect of Berberine on the Prevention and/or Treatment on Cyclophosphamide-Induced Testicular Damage in Rats. Osmangazi Tıp Dergisi. 2023; 45(2): 161 - 171. 10.20515/otd.1180404
IEEE	altuntaş h,Özdemir M,Harmanci N,yigitaslan s,Sahinturk V "The Effect of Berberine on the Prevention and/or Treatment on Cyclophosphamide-Induced Testicular Damage in Rats." Osmangazi Tıp Dergisi, 45, ss.161 - 171, 2023. 10.20515/otd.1180404
ISNAD	altuntaş, hakan vd. "The Effect of Berberine on the Prevention and/or Treatment on Cyclophosphamide-Induced Testicular Damage in Rats". Osmangazi Tıp Dergisi 45/2 (2023), 161-171. https://doi.org/10.20515/otd.1180404