The Optimal Time of Peripheral Blood Hematopoietic Stem Cell Collection for Autologous Transplantation May Be Predicted By Immature Granulocytes and Nucleated Red Blood Cells

Kocaturk, Evin; GUNDUZ, Eren; kayadibi, huseyin; Üsküdar Teke, Hava; Alataş, İ. Özkan

doi:10.20515/otd.1210794

The Optimal Time of Peripheral Blood Hematopoietic Stem Cell Collection for Autologous Transplantation May Be Predicted By Immature Granulocytes and Nucleated Red Blood Cells

Evin KOCATÜRK, (Eskisehir Osmangazi Üniversitesi, Tıp Fakültesi, Tıbbi Biyokimya Anabilim Dalı, Eskişehir, Türkiye)

Eren GUNDUZ, (Eskişehir Osmangazi Üniversitesi, Tıp Fakültesi, Hematoloji Anabilim Dalı, Eskişehir, Türkiye)

Hüseyin KAYADİBİ, (Eskisehir Osmangazi Üniversitesi, Tıp Fakültesi, Tıbbi Biyokimya Anabilim Dalı, Eskişehir, Türkiye)

Hava ÜSKÜDAR TEKE, (Eskişehir Osmangazi Üniversitesi, Tıp Fakültesi, Hematoloji Anabilim Dalı, Eskişehir, Türkiye)

İbrahim Özkan ALATAŞ (Eskisehir Osmangazi Üniversitesi, Tıp Fakültesi, Tıbbi Biyokimya Anabilim Dalı, Eskişehir, Türkiye)

Osmangazi Tıp Dergisi

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Yıl: 2023 Cilt: 45 Sayı: 2 Sayfa Aralığı: 230 - 235 Metin Dili: İngilizce DOI: 10.20515/otd.1210794 İndeks Tarihi: 06-09-2023

The Optimal Time of Peripheral Blood Hematopoietic Stem Cell Collection for Autologous Transplantation May Be Predicted By Immature Granulocytes and Nucleated Red Blood Cells

Öz:

The time of initiating stem cell collection is very important for successful transplantation and is determined according to peripheral blood CD34+ cell count analysed by flow cytometry. In this study, we aimed to find the role of new complete blood count parameters to determine the optimal time of stem cell collection. Eighty-six patients who underwent stem cell mobilization were included in the study. Peripheral blood CD34+ cells and complete blood count were analysed on the same day. Patients with peripheral blood CD 34+ cell counts ≤ 20/μL and > 20/μL were determined as Group 1 and Group 2, respectively. The difference of CBC parameters between 2 groups and the relationship of these parameters with peripheral blood CD34+ cell counts were evaluated. It was found that immature granulocytes ratio, nucleated red blood cells ratio and count were positively correlated with peripheral blood CD34+ cell count and these parameters were significantly different between two groups (P< 0.001, P= 0.011 and P= 0.012, respectively). The new index formulated by using immature granulocytes and nucleated red blood cells ratios was significantly different between two groups (P<0.001), and had the highest diagnostic accuracy to determine the time of stem cell collection (AUC= 0.766). Immature granulocytes and nucleated red blood cells can be used to determine the time of peripheral blood stem cell collection faster and cheaper compared to flow cytometry.

Anahtar Kelime: Autologous transplantation Stem cell mobilization CD34+ cells Immature granulocytes Nucleated red blood cells

Otolog Nakil İçin Periferik Kan Hematopoietik Kök Hücre Toplamanın Optimal Zamanı, Olgunlaşmamış Granülositler ve Çekirdekli Kırmızı Kan Hücreleri Tarafından Tahmin Edilebilir

Öz:

Başarılı bir nakil için kök hücre toplamaya başlama zamanı çok önemlidir ve bu zaman akış sitometrisi ile analiz edilen periferik kan CD34+ hücre sayısına göre belirlenir. Bu çalışmada, kök hücre toplamanın optimal zamanını belirlemek için yeni tam kan sayımı parametrelerinin rolünü bulmayı amaçladık. Çalışmaya kök hücre mobilizasyonu uygulanan 86 hasta dahil edildi. Aynı gün periferik kan CD34+ hücreleri ve tam kan sayımı yapıldı. Periferik kan CD 34+ hücre sayısı ≤ 20/μL ve > 20/μL olan hastalar sırasıyla Grup 1 ve Grup 2 olarak belirlendi. İki grup arasındaki tam kan sayımı parametreleri farkı ve bu parametrelerin periferik kan CD34+ hücre sayısı ile ilişkisi değerlendirildi. İmmatür granülosit oranı, çekirdekli eritrosit oranı ve sayısının periferik kan CD34+ hücre sayısı ile pozitif korelasyon gösterdiği ve bu parametrelerin iki grup arasında anlamlı olarak farklı olduğu bulundu (sırasıyla P< 0.001, P= 0.011 ve P= 0.012). Olgunlaşmamış granülositler ve çekirdekli kırmızı kan hücreleri oranları kullanılarak formüle edilen yeni indeks, iki grup arasında önemli ölçüde farklıydı (P<0.001) ve kök hücre toplama zamanını belirlemek için en yüksek tanı doğruluğuna sahipti (AUC= 0.766). İmmatür granülositler ve çekirdekli kırmızı kan hücreleri, akış sitometrisine göre periferik kan kök hücre toplama zamanını daha hızlı ve daha ucuza belirlemek için kullanılabilir.

Anahtar Kelime: Autologous transplantation Stem cell mobilization CD34+ cells Immature granulocytes Nucleated red blood cells

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	Kocaturk E, GUNDUZ E, kayadibi h, Üsküdar Teke H, Alataş İ (2023). The Optimal Time of Peripheral Blood Hematopoietic Stem Cell Collection for Autologous Transplantation May Be Predicted By Immature Granulocytes and Nucleated Red Blood Cells. , 230 - 235. 10.20515/otd.1210794
Chicago	Kocaturk Evin,GUNDUZ Eren,kayadibi huseyin,Üsküdar Teke Hava,Alataş İ. Özkan The Optimal Time of Peripheral Blood Hematopoietic Stem Cell Collection for Autologous Transplantation May Be Predicted By Immature Granulocytes and Nucleated Red Blood Cells. (2023): 230 - 235. 10.20515/otd.1210794
MLA	Kocaturk Evin,GUNDUZ Eren,kayadibi huseyin,Üsküdar Teke Hava,Alataş İ. Özkan The Optimal Time of Peripheral Blood Hematopoietic Stem Cell Collection for Autologous Transplantation May Be Predicted By Immature Granulocytes and Nucleated Red Blood Cells. , 2023, ss.230 - 235. 10.20515/otd.1210794
AMA	Kocaturk E,GUNDUZ E,kayadibi h,Üsküdar Teke H,Alataş İ The Optimal Time of Peripheral Blood Hematopoietic Stem Cell Collection for Autologous Transplantation May Be Predicted By Immature Granulocytes and Nucleated Red Blood Cells. . 2023; 230 - 235. 10.20515/otd.1210794
Vancouver	Kocaturk E,GUNDUZ E,kayadibi h,Üsküdar Teke H,Alataş İ The Optimal Time of Peripheral Blood Hematopoietic Stem Cell Collection for Autologous Transplantation May Be Predicted By Immature Granulocytes and Nucleated Red Blood Cells. . 2023; 230 - 235. 10.20515/otd.1210794
IEEE	Kocaturk E,GUNDUZ E,kayadibi h,Üsküdar Teke H,Alataş İ "The Optimal Time of Peripheral Blood Hematopoietic Stem Cell Collection for Autologous Transplantation May Be Predicted By Immature Granulocytes and Nucleated Red Blood Cells." , ss.230 - 235, 2023. 10.20515/otd.1210794
ISNAD	Kocaturk, Evin vd. "The Optimal Time of Peripheral Blood Hematopoietic Stem Cell Collection for Autologous Transplantation May Be Predicted By Immature Granulocytes and Nucleated Red Blood Cells". (2023), 230-235. https://doi.org/10.20515/otd.1210794

APA	Kocaturk E, GUNDUZ E, kayadibi h, Üsküdar Teke H, Alataş İ (2023). The Optimal Time of Peripheral Blood Hematopoietic Stem Cell Collection for Autologous Transplantation May Be Predicted By Immature Granulocytes and Nucleated Red Blood Cells. Osmangazi Tıp Dergisi, 45(2), 230 - 235. 10.20515/otd.1210794
Chicago	Kocaturk Evin,GUNDUZ Eren,kayadibi huseyin,Üsküdar Teke Hava,Alataş İ. Özkan The Optimal Time of Peripheral Blood Hematopoietic Stem Cell Collection for Autologous Transplantation May Be Predicted By Immature Granulocytes and Nucleated Red Blood Cells. Osmangazi Tıp Dergisi 45, no.2 (2023): 230 - 235. 10.20515/otd.1210794
MLA	Kocaturk Evin,GUNDUZ Eren,kayadibi huseyin,Üsküdar Teke Hava,Alataş İ. Özkan The Optimal Time of Peripheral Blood Hematopoietic Stem Cell Collection for Autologous Transplantation May Be Predicted By Immature Granulocytes and Nucleated Red Blood Cells. Osmangazi Tıp Dergisi, vol.45, no.2, 2023, ss.230 - 235. 10.20515/otd.1210794
AMA	Kocaturk E,GUNDUZ E,kayadibi h,Üsküdar Teke H,Alataş İ The Optimal Time of Peripheral Blood Hematopoietic Stem Cell Collection for Autologous Transplantation May Be Predicted By Immature Granulocytes and Nucleated Red Blood Cells. Osmangazi Tıp Dergisi. 2023; 45(2): 230 - 235. 10.20515/otd.1210794
Vancouver	Kocaturk E,GUNDUZ E,kayadibi h,Üsküdar Teke H,Alataş İ The Optimal Time of Peripheral Blood Hematopoietic Stem Cell Collection for Autologous Transplantation May Be Predicted By Immature Granulocytes and Nucleated Red Blood Cells. Osmangazi Tıp Dergisi. 2023; 45(2): 230 - 235. 10.20515/otd.1210794
IEEE	Kocaturk E,GUNDUZ E,kayadibi h,Üsküdar Teke H,Alataş İ "The Optimal Time of Peripheral Blood Hematopoietic Stem Cell Collection for Autologous Transplantation May Be Predicted By Immature Granulocytes and Nucleated Red Blood Cells." Osmangazi Tıp Dergisi, 45, ss.230 - 235, 2023. 10.20515/otd.1210794
ISNAD	Kocaturk, Evin vd. "The Optimal Time of Peripheral Blood Hematopoietic Stem Cell Collection for Autologous Transplantation May Be Predicted By Immature Granulocytes and Nucleated Red Blood Cells". Osmangazi Tıp Dergisi 45/2 (2023), 230-235. https://doi.org/10.20515/otd.1210794