DEVELOPMENT OF AMPHOTERICIN-B LOADED NANOPARTICLES AND EVALUATION THE ANTIMICROBIAL POTENCY
Yıl: 2023 Cilt: 47 Sayı: 3 Sayfa Aralığı: 1033 - 1040 Metin Dili: İngilizce DOI: 10.33483/jfpau.1352203 İndeks Tarihi: 26-09-2023
DEVELOPMENT OF AMPHOTERICIN-B LOADED NANOPARTICLES AND EVALUATION THE ANTIMICROBIAL POTENCY
Öz: Objective: The aim of this study is the development of Amphotericin B loaded polymeric nanoparticles and the determination of the potency of Amphotericin B nanoformulation samples and commercially supplied Amphotericin B samples in comparison with reference Amphotericin B standard, according to the protocol detailed in the United States Pharmacopoeia. Material and Method: Amphotericin B nanoparticles were fabricated using single emulsion method. The comparison of the potencies of the AmB nanoformulation and commercial Amphotericin B with the antimicrobial potency of the reference Amphotericin B standard was performed using the disk diffusion method specified in the United States Pharmacopeia. Result and Discussion: Amphotericin B loaded poly(ethylene glycol)-b-poly(ɛ-caprolactone) nanoparticles successfully developed having the average hydrodynamic diameter of 215.14±0.72 nm and PDI value of 0.18±0.02. The Amphotericin B encapsulation efficiency, which was determined using an HPLC method, was 66.4±1.42%. The % potency of commercial Amphotericin B was calculated as 95.7%, while the % potency of the nanoformulation of Amphotericin B was calculated as 99.1%, indicating the favor of utilizing polymeric nanoparticles as delivery systems.
Anahtar Kelime: AMFOTERİSİN-B ENKAPSÜLE EDİLMİŞ NANOPARTİKÜLLERİN ANTİMİKROBİYAL POTENSİNİN DEĞERLENDİRİLMESİ
Öz: Amaç: Bu çalışmanın amacı, Amfoterisin B yüklü polimerik nanopartiküllerin geliştirilmesi ve Amfoterisin B nanoformülasyon örneklerinin ve ticari olarak temin edilen Amfoterisin B örneklerinin potensinin, Amerika Birleşik Devletleri Farmakopesi'nde ayrıntılı olarak açıklanan protokole göre referans Amfoterisin B standardı ile karşılaştırmalı olarak belirlenmesidir. Gereç ve Yöntem: Amfoterisin B nanopartikülleri tek emülsiyon yöntemi kullanılarak üretilmiştir. Referans Amfoterisin B standardının antimikrobiyal potensi ile ticari Amfoterisin B ve Amfoterisin B'nin nanoformülasyonunun potenslerinin kıyaslanması, Amerika Birleşik Devletleri Farmakopesinde belirtilen disk difüzyon yöntemi kullanılarak gerçekleştirilmiştir. Sonuç ve Tartışma: Ortalama hidrodinamik çapı 215.14±0.72 nm ve PDI değeri 0.18±0.02 olan Amfoterisin B yüklü poli(etilen glikol)-b-poli(ɛ-kaprolakton) nanopartikülleri başarıyla geliştirilmiştir. HPLC yöntemi kullanılarak belirlenen Amfoterisin B enkapsülasyon etkinliği %66.4±1.42 olarak bulunmuştur. Ticari Amfoterisin B'nin % potensi %95.7 olarak hesaplanırken, Amfoterisin B'nin nanoformülasyonunun % potensi %99.1 olarak hesaplanmış olup bu bulgu, taşıyıcı sistem olarak polimerik nanopartiküllerin kullanılmasının avantajını ortaya koymaktadır.
Anahtar Kelime: Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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| APA | Oz U, Rizvanoglu S, Abamor E, CENGİZ G, Berber H, DERMAN S, eryilmaz m, Bozkır A (2023). DEVELOPMENT OF AMPHOTERICIN-B LOADED NANOPARTICLES AND EVALUATION THE ANTIMICROBIAL POTENCY. , 1033 - 1040. 10.33483/jfpau.1352203 |
| Chicago | Oz Umut Can,Rizvanoglu Suna Sibel,Abamor Emrah Sefik,CENGİZ Gökhan,Berber Hale,DERMAN Serap,eryilmaz mujde,Bozkır Asuman DEVELOPMENT OF AMPHOTERICIN-B LOADED NANOPARTICLES AND EVALUATION THE ANTIMICROBIAL POTENCY. (2023): 1033 - 1040. 10.33483/jfpau.1352203 |
| MLA | Oz Umut Can,Rizvanoglu Suna Sibel,Abamor Emrah Sefik,CENGİZ Gökhan,Berber Hale,DERMAN Serap,eryilmaz mujde,Bozkır Asuman DEVELOPMENT OF AMPHOTERICIN-B LOADED NANOPARTICLES AND EVALUATION THE ANTIMICROBIAL POTENCY. , 2023, ss.1033 - 1040. 10.33483/jfpau.1352203 |
| AMA | Oz U,Rizvanoglu S,Abamor E,CENGİZ G,Berber H,DERMAN S,eryilmaz m,Bozkır A DEVELOPMENT OF AMPHOTERICIN-B LOADED NANOPARTICLES AND EVALUATION THE ANTIMICROBIAL POTENCY. . 2023; 1033 - 1040. 10.33483/jfpau.1352203 |
| Vancouver | Oz U,Rizvanoglu S,Abamor E,CENGİZ G,Berber H,DERMAN S,eryilmaz m,Bozkır A DEVELOPMENT OF AMPHOTERICIN-B LOADED NANOPARTICLES AND EVALUATION THE ANTIMICROBIAL POTENCY. . 2023; 1033 - 1040. 10.33483/jfpau.1352203 |
| IEEE | Oz U,Rizvanoglu S,Abamor E,CENGİZ G,Berber H,DERMAN S,eryilmaz m,Bozkır A "DEVELOPMENT OF AMPHOTERICIN-B LOADED NANOPARTICLES AND EVALUATION THE ANTIMICROBIAL POTENCY." , ss.1033 - 1040, 2023. 10.33483/jfpau.1352203 |
| ISNAD | Oz, Umut Can vd. "DEVELOPMENT OF AMPHOTERICIN-B LOADED NANOPARTICLES AND EVALUATION THE ANTIMICROBIAL POTENCY". (2023), 1033-1040. https://doi.org/10.33483/jfpau.1352203 |
| APA | Oz U, Rizvanoglu S, Abamor E, CENGİZ G, Berber H, DERMAN S, eryilmaz m, Bozkır A (2023). DEVELOPMENT OF AMPHOTERICIN-B LOADED NANOPARTICLES AND EVALUATION THE ANTIMICROBIAL POTENCY. Ankara Üniversitesi Eczacılık Fakültesi Dergisi, 47(3), 1033 - 1040. 10.33483/jfpau.1352203 |
| Chicago | Oz Umut Can,Rizvanoglu Suna Sibel,Abamor Emrah Sefik,CENGİZ Gökhan,Berber Hale,DERMAN Serap,eryilmaz mujde,Bozkır Asuman DEVELOPMENT OF AMPHOTERICIN-B LOADED NANOPARTICLES AND EVALUATION THE ANTIMICROBIAL POTENCY. Ankara Üniversitesi Eczacılık Fakültesi Dergisi 47, no.3 (2023): 1033 - 1040. 10.33483/jfpau.1352203 |
| MLA | Oz Umut Can,Rizvanoglu Suna Sibel,Abamor Emrah Sefik,CENGİZ Gökhan,Berber Hale,DERMAN Serap,eryilmaz mujde,Bozkır Asuman DEVELOPMENT OF AMPHOTERICIN-B LOADED NANOPARTICLES AND EVALUATION THE ANTIMICROBIAL POTENCY. Ankara Üniversitesi Eczacılık Fakültesi Dergisi, vol.47, no.3, 2023, ss.1033 - 1040. 10.33483/jfpau.1352203 |
| AMA | Oz U,Rizvanoglu S,Abamor E,CENGİZ G,Berber H,DERMAN S,eryilmaz m,Bozkır A DEVELOPMENT OF AMPHOTERICIN-B LOADED NANOPARTICLES AND EVALUATION THE ANTIMICROBIAL POTENCY. Ankara Üniversitesi Eczacılık Fakültesi Dergisi. 2023; 47(3): 1033 - 1040. 10.33483/jfpau.1352203 |
| Vancouver | Oz U,Rizvanoglu S,Abamor E,CENGİZ G,Berber H,DERMAN S,eryilmaz m,Bozkır A DEVELOPMENT OF AMPHOTERICIN-B LOADED NANOPARTICLES AND EVALUATION THE ANTIMICROBIAL POTENCY. Ankara Üniversitesi Eczacılık Fakültesi Dergisi. 2023; 47(3): 1033 - 1040. 10.33483/jfpau.1352203 |
| IEEE | Oz U,Rizvanoglu S,Abamor E,CENGİZ G,Berber H,DERMAN S,eryilmaz m,Bozkır A "DEVELOPMENT OF AMPHOTERICIN-B LOADED NANOPARTICLES AND EVALUATION THE ANTIMICROBIAL POTENCY." Ankara Üniversitesi Eczacılık Fakültesi Dergisi, 47, ss.1033 - 1040, 2023. 10.33483/jfpau.1352203 |
| ISNAD | Oz, Umut Can vd. "DEVELOPMENT OF AMPHOTERICIN-B LOADED NANOPARTICLES AND EVALUATION THE ANTIMICROBIAL POTENCY". Ankara Üniversitesi Eczacılık Fakültesi Dergisi 47/3 (2023), 1033-1040. https://doi.org/10.33483/jfpau.1352203 |
