TY  - JOUR
TI  - DEVELOPMENT OF AMPHOTERICIN-B LOADED NANOPARTICLES AND EVALUATION THE ANTIMICROBIAL POTENCY
AB  - Objective: The aim of this study is the development of Amphotericin B loaded polymeric nanoparticles and the determination of the potency of Amphotericin B nanoformulation samples and commercially supplied Amphotericin B samples in comparison with reference Amphotericin B standard, according to the protocol detailed in the United States Pharmacopoeia. Material and Method: Amphotericin B nanoparticles were fabricated using single emulsion method. The comparison of the potencies of the AmB nanoformulation and commercial Amphotericin B with the antimicrobial potency of the reference Amphotericin B standard was performed using the disk diffusion method specified in the United States Pharmacopeia. Result and Discussion: Amphotericin B loaded poly(ethylene glycol)-b-poly(ɛ-caprolactone) nanoparticles successfully developed having the average hydrodynamic diameter of 215.14±0.72 nm and PDI value of 0.18±0.02. The Amphotericin B encapsulation efficiency, which was determined using an HPLC method, was 66.4±1.42%. The % potency of commercial Amphotericin B was calculated as 95.7%, while the % potency of the nanoformulation of Amphotericin B was calculated as 99.1%, indicating the favor of utilizing polymeric nanoparticles as delivery systems.
AU  - Oz, Umut Can
AU  - Rizvanoglu, Suna Sibel
AU  - Abamor, Emrah Sefik
AU  - CENGİZ, Gökhan
AU  - Berber, Hale
AU  - DERMAN, Serap
AU  - eryilmaz, mujde
AU  - Bozkır, Asuman
DO  - 10.33483/jfpau.1352203
PY  - 2023
JO  - Ankara Üniversitesi Eczacılık Fakültesi Dergisi
VL  - 47
IS  - 3
SN  - 1015-3918
SP  - 1033
EP  - 1040
DB  - TRDizin
UR  - http://search/yayin/detay/1197158
ER  -