The Effect of Curcumin on Penile Fibrotic Plaque in Rats with Experimental Peyronie’s Disease
Yıl: 2023 Cilt: 3 Sayı: 3 Sayfa Aralığı: 102 - 108 Metin Dili: İngilizce DOI: 10.5505/GJU.2023.62207 İndeks Tarihi: 26-09-2023
The Effect of Curcumin on Penile Fibrotic Plaque in Rats with Experimental Peyronie’s Disease
Öz: Objective: No effective medical approach for the treatment of Peyronie’s disease (PD) has to date been described. This study was intended to evaluate the antifibrotic, antioxidant, and anti-inflammatory effects of curcumin on fibrotic tissue in the tunica albuginea (TA) in a rat model of PD. Materials and Methods: Twenty-four male Sprague Dawley rats aged 10 months were randomized into three groups (n = 8 in each). No PD model was induced in the control group. The PD+saline (PD+Ps) group received fibrin injection, followed two weeks later by saline administration by oral gavage for 14 days. The PD+Curcumin (PD+Cur) group received fibrin injection into the TA followed two weeks later by curcumin administration by oral gavage for 14 days. At the end of the experiment, fibrotic activity was evaluated using stereological and histopathological methods. Transforming growth factor-β1 (TGF-β1), one of the most fibrogenic cytokines, was evaluated using immunohistochemistry with an anti-TGF-β1 rabbit monoclonal antibody. Results: Stereological analysis revealed significantly greater Peyronie-like plaque areas in the TA in the PD+Ps group than in the control and PD+Cur groups (p<0.0001). No significant difference was observed between the control and PD+Cur groups (p=0.35). The PD+Ps group exhibited strong TGFβ1 immunoreactivity with increased expression in the collagenous connective tissues and fibroblasts around the TA. Conclusion: Curcumin reduced fibrotic tissue in the TA and may represent a novel therapeutic option in the treatment of PD.
Anahtar Kelime: Deneysel Peyronie Hastalığı Oluşturulan Ratlarda Kurkuminin Penil Fibrotik Plak Üzerine Etkisi
Öz: Amaç: Bugüne kadar Peyronie hastalığı’nın (PH) tedavisi için etkili bir medikal tedavi tanımlanmamıştır. Bu çalışmada, deneysel olarak PH modeli oluşturulan ratların tunika albuginealarında (TA) oluşan fibrotik doku üzerine kurkuminin antiinflamatuar, antifibrotik ve antioksidan etkilerinin değerlendirmesi amaçlanmıştır. Gereçler ve Yöntemler: On aylık 24 adet erkek Sprague Dawley cinsi rat, eşit bir şekilde üç gruba randomize edildi. Kontrol grubuna deneysel PH modeli uygulanmadı. PH+salin (PD+Ps) grubuna fibrin enjeksiyonu yapıldı ve ardından iki hafta sonra 14 gün boyunca oral gavaj ile salin verildi. PH+Kurkumin (PH+Kur) grubuna TA’ya fibrin enjeksiyonu yapılarak PH modeli oluşturuldu ve ardından iki hafta sonra 14 gün boyunca oral gavaj ile kurkumin verildi. Deneyin sonunda fibrotik aktivite stereolojik ve histopatolojik yöntemler kullanılarak değerlendirildi. En fibrojenik sitokinlerden biri olan Transforming büyüme faktörü-β1 (TGF-β1), tavşan monoklonal antikoru olan anti-TGF-β1 kullanılarak immünohistokimyasal olarak ölçüldü. Bulgular: Stereolojik analizde PH+Ps grubunda, kontrol ve PH+Kur gruplarına göre anlamlı olarak TA’da daha fazla Peyronie benzeri plakların ortaya çıktığı görüldü (p<0,0001). Kontrol ve PH+Kur grupları arasında anlamlı fark izlenmedi (p=0,35). PH+Ps grubunda TA çevresindeki kollajenöz bağ dokularda ve fibroblastlarda güçlü TGF-β1 immün reaktivitesi izlendi. Sonuç: Çalışmamızda kurkuminin TA’daki fibrotik dokuyu azalttığı görülmüştür. Bu nedenle kurkumin PH tedavisinde yeni bir terapötik seçenek olabilir.
Anahtar Kelime: Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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| APA | Ulubay M, KAYA A, KAPLAN A, Tüfekci K, Akdeniz E, Kaplan S (2023). The Effect of Curcumin on Penile Fibrotic Plaque in Rats with Experimental Peyronie’s Disease. , 102 - 108. 10.5505/GJU.2023.62207 |
| Chicago | Ulubay Mahmut,KAYA AYŞENUR,KAPLAN Arife Ahsen,Tüfekci Kıymet Kübra,Akdeniz Ekrem,Kaplan Suleyman The Effect of Curcumin on Penile Fibrotic Plaque in Rats with Experimental Peyronie’s Disease. (2023): 102 - 108. 10.5505/GJU.2023.62207 |
| MLA | Ulubay Mahmut,KAYA AYŞENUR,KAPLAN Arife Ahsen,Tüfekci Kıymet Kübra,Akdeniz Ekrem,Kaplan Suleyman The Effect of Curcumin on Penile Fibrotic Plaque in Rats with Experimental Peyronie’s Disease. , 2023, ss.102 - 108. 10.5505/GJU.2023.62207 |
| AMA | Ulubay M,KAYA A,KAPLAN A,Tüfekci K,Akdeniz E,Kaplan S The Effect of Curcumin on Penile Fibrotic Plaque in Rats with Experimental Peyronie’s Disease. . 2023; 102 - 108. 10.5505/GJU.2023.62207 |
| Vancouver | Ulubay M,KAYA A,KAPLAN A,Tüfekci K,Akdeniz E,Kaplan S The Effect of Curcumin on Penile Fibrotic Plaque in Rats with Experimental Peyronie’s Disease. . 2023; 102 - 108. 10.5505/GJU.2023.62207 |
| IEEE | Ulubay M,KAYA A,KAPLAN A,Tüfekci K,Akdeniz E,Kaplan S "The Effect of Curcumin on Penile Fibrotic Plaque in Rats with Experimental Peyronie’s Disease." , ss.102 - 108, 2023. 10.5505/GJU.2023.62207 |
| ISNAD | Ulubay, Mahmut vd. "The Effect of Curcumin on Penile Fibrotic Plaque in Rats with Experimental Peyronie’s Disease". (2023), 102-108. https://doi.org/10.5505/GJU.2023.62207 |
| APA | Ulubay M, KAYA A, KAPLAN A, Tüfekci K, Akdeniz E, Kaplan S (2023). The Effect of Curcumin on Penile Fibrotic Plaque in Rats with Experimental Peyronie’s Disease. Grand journal of urology (Online), 3(3), 102 - 108. 10.5505/GJU.2023.62207 |
| Chicago | Ulubay Mahmut,KAYA AYŞENUR,KAPLAN Arife Ahsen,Tüfekci Kıymet Kübra,Akdeniz Ekrem,Kaplan Suleyman The Effect of Curcumin on Penile Fibrotic Plaque in Rats with Experimental Peyronie’s Disease. Grand journal of urology (Online) 3, no.3 (2023): 102 - 108. 10.5505/GJU.2023.62207 |
| MLA | Ulubay Mahmut,KAYA AYŞENUR,KAPLAN Arife Ahsen,Tüfekci Kıymet Kübra,Akdeniz Ekrem,Kaplan Suleyman The Effect of Curcumin on Penile Fibrotic Plaque in Rats with Experimental Peyronie’s Disease. Grand journal of urology (Online), vol.3, no.3, 2023, ss.102 - 108. 10.5505/GJU.2023.62207 |
| AMA | Ulubay M,KAYA A,KAPLAN A,Tüfekci K,Akdeniz E,Kaplan S The Effect of Curcumin on Penile Fibrotic Plaque in Rats with Experimental Peyronie’s Disease. Grand journal of urology (Online). 2023; 3(3): 102 - 108. 10.5505/GJU.2023.62207 |
| Vancouver | Ulubay M,KAYA A,KAPLAN A,Tüfekci K,Akdeniz E,Kaplan S The Effect of Curcumin on Penile Fibrotic Plaque in Rats with Experimental Peyronie’s Disease. Grand journal of urology (Online). 2023; 3(3): 102 - 108. 10.5505/GJU.2023.62207 |
| IEEE | Ulubay M,KAYA A,KAPLAN A,Tüfekci K,Akdeniz E,Kaplan S "The Effect of Curcumin on Penile Fibrotic Plaque in Rats with Experimental Peyronie’s Disease." Grand journal of urology (Online), 3, ss.102 - 108, 2023. 10.5505/GJU.2023.62207 |
| ISNAD | Ulubay, Mahmut vd. "The Effect of Curcumin on Penile Fibrotic Plaque in Rats with Experimental Peyronie’s Disease". Grand journal of urology (Online) 3/3 (2023), 102-108. https://doi.org/10.5505/GJU.2023.62207 |
