CLINICOPATHOLOGIC VALUE OF IMMUNOHISTOCHEMICAL PINCH/LIMS-1 ANTIBODY EXPRESSION IN OVARIAN SEROUS CARCINOMA AND BORDERLINE SEROUS TUMOR
Yıl: 2023 Cilt: 30 Sayı: 3 Sayfa Aralığı: 592 - 601 Metin Dili: İngilizce DOI: 10.17343/sdutfd.1349330 İndeks Tarihi: 02-10-2023
CLINICOPATHOLOGIC VALUE OF IMMUNOHISTOCHEMICAL PINCH/LIMS-1 ANTIBODY EXPRESSION IN OVARIAN SEROUS CARCINOMA AND BORDERLINE SEROUS TUMOR
Öz: Objective Particularly interesting cys-his rich protein (PINCH/ LIMS-1), a protein implicated in cell adhesion, is assumed to oversee the development and invasion of cancer cells in tumors and tumor-associated stroma. This study aimed to assess PINCH-1 expression in serous borderline tumor (SBT) and serous carcinoma (SC) in the tumor and peritumoral stroma and scrutinize any associations between its expression and various clinical and pathological parameters. Material and Method In this study, the expression of the PINCH-1 antibody was analyzed in 21 cases of SBT and 89 cases of SC using the indirect immunoperoxidase technique with streptavidin/HRP-biotin. The staining pattern of PINCH-1 in the tumor and peritumoral stroma was evaluated using a semiquantitative scoring method. The staining procedure used in the study allowed for the accurate identification of PINCH-1 expression, and the data obtained through the semiquantitative scoring method provided a reliable of assessing the degree and intensity of PINCH-1 staining. Thus, the correlation between PINCH-1 expression and various pathologic factors such as patient age, tumor size, FIGO stage, intra-abdominal washing cytology, capsule invasion, tumor location in the ovary, tumor grade, and cancer antigen 125 (CA125) levels at the time of diagnosis was examined. Results The study found that PINCH-1 was more prevalent in cases of SC than in SBT cases. The tumors in SC cases had stronger staining than those in SBT cases (p<0.001). The study also found a positive correlation between the diffusiveness and intensity of PINCH-1 staining in the tissue surrounding the tumor and whether the tumor was located on one or both sides of the ovaries (p = 0.038 for diffusiveness, p = 0.024 for intensity). However, there was a negative correlation between PINCH-1 staining and tumor size (p=0.019 for diffusiveness, p=0.007 for intensity). Furthermore, the intensity of PINCH-1 tumor staining exhibited statistical significance in Figo stage and tumor grades as these increased (p = 0.032 and p = 0.001, respectively). Conclusion The results of this study indicate that SCs exhibit a higher level of PINCH-1 staining intensity than SBTs. Furthermore, an increase in the FIGO stage and tumor grade is associated with increased intensity of PINCH-1 staining in the tumor tissue. Additionally, the diffusiveness and intensity of PINCH-1 staining in the peritumoral stroma is more remarkable in cases of bilateral tumors but is inversely correlated with tumor size. The observed association between PINCH-1 expression and important clinicopathologic factors suggests that this molecule may be involved in developing serous ovarian cancer. Overall, these findings may contribute to a better understanding of the role of PINCH-1 in ovarian tumorigenesis and may have implications for developing novel therapeutic strategies targeting this pathway.
Anahtar Kelime: OVER SERÖZ KARSİNOMUNDA VE BORDERLİNE SERÖZ TÜMÖRDE İMMÜNHİSTOKİMYASAL PINCH/LIMS-1 ANTİKOR EKSPRESYONUNUN KLİNİKOPATOLOJİK DEĞERİ
Öz: Amaç Özellikle ilginç cys-his zengin proteinin (PINCH/LIMS- 1), tümörlerdeki ve tümörle ilişkili stromadaki kanser hücrelerinin gelişimini ve yayılımını denetlediği var- sayılmaktadır. Bu çalışmanın amacı, seröz borderline tümör (SBT) ve seröz karsinomda (SC) tümör ve pe- ritümöral stromadaki PINCH-1 ekspresyonunu değer- lendirmek ve ekspresyonu ile çeşitli klinik ve patolojik parametreler arasındaki ilişkileri incelemektir. Gereç ve Yöntem Yirmi bir SBT ve 89 SK vakasında PINCH-1 antikoru- nun ekspresyonu streptavidin/HRP-biotin ile indirekt immünoperoksidaz tekniği kullanılarak analiz edil- miştir. PINCH-1'in tümör ve peritümöral stromadaki boyanma paterni semikantitatif skorlama yöntemi kullanılarak değerlendirilmiştir. Çalışmada kullanılan boyama yöntemi PINCH-1 ekspresyonunun tanım- lanmasına olanak sağlamış ve semikantitatif skorla- ma yöntemiyle PINCH-1 boyanmasının yaygınlığı ve yoğunluğu değerlendirilmiştir. Böylece, PINCH-1 eks- presyonu ile hasta yaşı, tümör boyutu, FIGO evresi, intraabdominal yıkama sitolojisi, kapsül invazyonu, tümör yerleşimi, tümör derecesi ve tanı anındaki kan- ser antijen 125 (CA125) seviyeleri gibi çeşitli klinik ve patolojik faktörler arasındaki korelasyon incelenmiştir. Bulgular Çalışmada PINCH-1'in SK vakalarında SBT vakaları- na göre daha yaygın olduğu bulunmuştur. SK’ler SBT vakalarındakilere göre daha güçlü boyanma göster- miştir (p<0,001). Çalışmada ayrıca, tümörü çevrele- yen dokudaki PINCH-1 boyanmasının yaygınlığı ve yoğunluğu ile tümörün tek ya da bilateral overde yer alması arasında pozitif bir korelasyon bulunmuştur (dağılım için p = 0,038, yoğunluk için p = 0,024). Bu- nunla birlikte, PINCH-1 boyanması ile tümör boyutu arasında negatif bir korelasyon vardı (yaygınlık için p=0.019, yoğunluk için p=0.007). Ayrıca, PINCH-1 tü- mör boyanmasının yoğunluğu, FIGO evresi ve tümör dereceleri arttıkça istatistiksel anlamlılık sergilemiştir (sırasıyla p = 0,032 ve p = 0,001). Sonuç Bu çalışmanın sonuçları, SK'lerin SBT'lere göre daha yüksek düzeyde PINCH-1 boyanma yoğunluğu ser- gilediğini göstermektedir. Ayrıca, FIGO evresindeki ve tümör derecesindeki artış, tümör dokusunda artan PINCH-1 boyanma yoğunluğu ile ilişkilidir. Peritümö- ral stromadaki PINCH-1 boyanmasının yayılımı ve yo- ğunluğu bilateral tümör vakalarında daha dikkat çeki- cidir, ancak tümör boyutuyla ters orantılıdır. PINCH-1 ekspresyonu ile önemli klinikopatolojik faktörler ara- sında gözlenen ilişki, bu molekülün seröz over kanse- ri gelişiminde rol oynayabileceğini düşündürmektedir. Çalışmamız PINCH-1'in tümörogenezdeki rolünün daha iyi anlaşılmasına katkıda bulunabilir ve bu yolağı hedefleyen yeni terapötik stratejilerin geliştirilmesi için yol gösterici olabilir.
Anahtar Kelime: Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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| APA | Ertunc O, Erdem O, Kaymak Z (2023). CLINICOPATHOLOGIC VALUE OF IMMUNOHISTOCHEMICAL PINCH/LIMS-1 ANTIBODY EXPRESSION IN OVARIAN SEROUS CARCINOMA AND BORDERLINE SEROUS TUMOR. , 592 - 601. 10.17343/sdutfd.1349330 |
| Chicago | Ertunc Onur,Erdem Ozlem,Kaymak Zumrut Arda CLINICOPATHOLOGIC VALUE OF IMMUNOHISTOCHEMICAL PINCH/LIMS-1 ANTIBODY EXPRESSION IN OVARIAN SEROUS CARCINOMA AND BORDERLINE SEROUS TUMOR. (2023): 592 - 601. 10.17343/sdutfd.1349330 |
| MLA | Ertunc Onur,Erdem Ozlem,Kaymak Zumrut Arda CLINICOPATHOLOGIC VALUE OF IMMUNOHISTOCHEMICAL PINCH/LIMS-1 ANTIBODY EXPRESSION IN OVARIAN SEROUS CARCINOMA AND BORDERLINE SEROUS TUMOR. , 2023, ss.592 - 601. 10.17343/sdutfd.1349330 |
| AMA | Ertunc O,Erdem O,Kaymak Z CLINICOPATHOLOGIC VALUE OF IMMUNOHISTOCHEMICAL PINCH/LIMS-1 ANTIBODY EXPRESSION IN OVARIAN SEROUS CARCINOMA AND BORDERLINE SEROUS TUMOR. . 2023; 592 - 601. 10.17343/sdutfd.1349330 |
| Vancouver | Ertunc O,Erdem O,Kaymak Z CLINICOPATHOLOGIC VALUE OF IMMUNOHISTOCHEMICAL PINCH/LIMS-1 ANTIBODY EXPRESSION IN OVARIAN SEROUS CARCINOMA AND BORDERLINE SEROUS TUMOR. . 2023; 592 - 601. 10.17343/sdutfd.1349330 |
| IEEE | Ertunc O,Erdem O,Kaymak Z "CLINICOPATHOLOGIC VALUE OF IMMUNOHISTOCHEMICAL PINCH/LIMS-1 ANTIBODY EXPRESSION IN OVARIAN SEROUS CARCINOMA AND BORDERLINE SEROUS TUMOR." , ss.592 - 601, 2023. 10.17343/sdutfd.1349330 |
| ISNAD | Ertunc, Onur vd. "CLINICOPATHOLOGIC VALUE OF IMMUNOHISTOCHEMICAL PINCH/LIMS-1 ANTIBODY EXPRESSION IN OVARIAN SEROUS CARCINOMA AND BORDERLINE SEROUS TUMOR". (2023), 592-601. https://doi.org/10.17343/sdutfd.1349330 |
| APA | Ertunc O, Erdem O, Kaymak Z (2023). CLINICOPATHOLOGIC VALUE OF IMMUNOHISTOCHEMICAL PINCH/LIMS-1 ANTIBODY EXPRESSION IN OVARIAN SEROUS CARCINOMA AND BORDERLINE SEROUS TUMOR. Süleyman Demirel Üniversitesi Tıp Fakültesi Dergisi, 30(3), 592 - 601. 10.17343/sdutfd.1349330 |
| Chicago | Ertunc Onur,Erdem Ozlem,Kaymak Zumrut Arda CLINICOPATHOLOGIC VALUE OF IMMUNOHISTOCHEMICAL PINCH/LIMS-1 ANTIBODY EXPRESSION IN OVARIAN SEROUS CARCINOMA AND BORDERLINE SEROUS TUMOR. Süleyman Demirel Üniversitesi Tıp Fakültesi Dergisi 30, no.3 (2023): 592 - 601. 10.17343/sdutfd.1349330 |
| MLA | Ertunc Onur,Erdem Ozlem,Kaymak Zumrut Arda CLINICOPATHOLOGIC VALUE OF IMMUNOHISTOCHEMICAL PINCH/LIMS-1 ANTIBODY EXPRESSION IN OVARIAN SEROUS CARCINOMA AND BORDERLINE SEROUS TUMOR. Süleyman Demirel Üniversitesi Tıp Fakültesi Dergisi, vol.30, no.3, 2023, ss.592 - 601. 10.17343/sdutfd.1349330 |
| AMA | Ertunc O,Erdem O,Kaymak Z CLINICOPATHOLOGIC VALUE OF IMMUNOHISTOCHEMICAL PINCH/LIMS-1 ANTIBODY EXPRESSION IN OVARIAN SEROUS CARCINOMA AND BORDERLINE SEROUS TUMOR. Süleyman Demirel Üniversitesi Tıp Fakültesi Dergisi. 2023; 30(3): 592 - 601. 10.17343/sdutfd.1349330 |
| Vancouver | Ertunc O,Erdem O,Kaymak Z CLINICOPATHOLOGIC VALUE OF IMMUNOHISTOCHEMICAL PINCH/LIMS-1 ANTIBODY EXPRESSION IN OVARIAN SEROUS CARCINOMA AND BORDERLINE SEROUS TUMOR. Süleyman Demirel Üniversitesi Tıp Fakültesi Dergisi. 2023; 30(3): 592 - 601. 10.17343/sdutfd.1349330 |
| IEEE | Ertunc O,Erdem O,Kaymak Z "CLINICOPATHOLOGIC VALUE OF IMMUNOHISTOCHEMICAL PINCH/LIMS-1 ANTIBODY EXPRESSION IN OVARIAN SEROUS CARCINOMA AND BORDERLINE SEROUS TUMOR." Süleyman Demirel Üniversitesi Tıp Fakültesi Dergisi, 30, ss.592 - 601, 2023. 10.17343/sdutfd.1349330 |
| ISNAD | Ertunc, Onur vd. "CLINICOPATHOLOGIC VALUE OF IMMUNOHISTOCHEMICAL PINCH/LIMS-1 ANTIBODY EXPRESSION IN OVARIAN SEROUS CARCINOMA AND BORDERLINE SEROUS TUMOR". Süleyman Demirel Üniversitesi Tıp Fakültesi Dergisi 30/3 (2023), 592-601. https://doi.org/10.17343/sdutfd.1349330 |
