In this study, a simple thermoacoustic refrigerator system experiment set was established by using the design parameters in accordance with the relevant literature. In the experimental setup, a loudspeaker was used as acoustic power source and a suitable frequency value was determined for the system. Three kinds of stack materials with different thermal conductivity coefficients, respectively, mylar, cotton and glass wool were placed in the resonance tube. The temperature values at both ends of the stack material were measured by placed temperature gauges. In the measurements made within the same time period, the temperature differences were measured as 5.2°C for mylar, 4.7°C for cotton and 4.3°C for glass wool, respectively. It was determined that the highest temperature difference was in the mylar material.