The Preparation of Liposomal Formulations of Gentamicin and Ceftiofur Used in Veterinary Medicine

Hasan SUSAR, (Balıkesir Üniversitesi, Veteriner Fakültesi, Farmakoloji ve Toksikoloji Anabilim Dalı, Balıkesir, Türkiye)
Murat ÇELEBİ, (Balıkesir Üniversitesi, Savaştepe Meslek Yüksekokulu, Laboratuvar ve Veteriner Sağlığı Bölümü, Balıkesir, Türkiye)
Çağla ÇELEBİ, (Balıkesir Üniversitesi, Sağlık Bilimleri Enstitüsü, Farmakoloji ve Toksikoloji Anabilim Dalı, Balıkesir, Türkiye)
Özlem ÇOBAN, (Karadeniz Teknik Üniversitesi, Eczacılık Fakültesi, İlaç ve Farmasötik Teknoloji Uygulama ve Araştırma Merkezi, Farmasötik Teknoloji Bölümü, Trabzon, Türkiye)
Hüseyin ŞEN, (Balıkesir Üniversitesi, Sağlık Bilimleri Enstitüsü, Farmakoloji ve Toksikoloji Anabilim Dalı, Balıkesir, Türkiye)
İzzet KARAHAN (Balıkesir Üniversitesi, Veteriner Fakültesi, Farmakoloji ve Toksikoloji Anabilim Dalı, Balıkesir, Türkiye)
Balıkesir Sağlık Bilimleri Dergisi (BSBD)
1 0
Yıl: 2023 Cilt: 12 Sayı: 3 Sayfa Aralığı: 554 - 559 Metin Dili: İngilizce DOI: 10.53424/balikesirsbd.1262051 İndeks Tarihi: 09-10-2023

The Preparation of Liposomal Formulations of Gentamicin and Ceftiofur Used in Veterinary Medicine

Öz:
Objective: The aim of this study is to produce liposomal drugs that are scarcely found in veterinary medicine. Therefore, the current study was designed to produce liposomal formulations of ceftiofur and gentamicin for veterinary use and to perform their quality control studies. Materials and Methods: The Bangham Method was chosen for the preparation of liposomal formulations of gentamicin and ceftiofur. Results: The particle size, polydispersity index and zeta potential were found to be 3934.67 nm, 0.471, -10.3 mV for Ceftiofur-1 coded formulation, 4573.0 nm, 0.308, -9.9 mV for Ceftiofur-2 coded formulation, 479.4 nm, 0.437, -44.8 mV for Ceftiofur-3 coded formulation, respectively. The particle size, polydispersity index and zeta potential were found to be 5185.67 nm, 0.599, -6.5 mV for Gentamicin-1 coded formulation, 4228.0 nm, 0.505, -7.8 mV for Gentamicin-2 coded formulation, 2138.67 nm, 0.565, -6.5 mV for Gentamicin-3 coded formulation, respectively. The encapsulation efficiency of liposomal formulations containing ceftiofur; 82.85%, 95.74%, and 92.06% was found for ceftiofur-1, ceftiofur-2 and ceftiofur 3, respectively. Conclusion: The liposomal formulations of ceftiofur and gentamicin were successfully prepared and their quality control studies were carried out. It was concluded that pharmacokinetic/pharmacodynamic studies should be performed to evaluate the efficacy of liposomal formulations.
Anahtar Kelime: Drug Formulation Liposome Veterinary.

Veteriner Hekimlikte Kullanılan Gentamicin ve Seftiofur'un Lipozomal Formülasyonlarının Hazırlanması

Öz:
veteriner kullanım için seftiofur ve gentamisinin lipozomal formülasyonlarını üretmek ve kalite kontrol çalışmalarını yapmak hedefleriyle tasarlanmıştır. Gereç ve Yöntem: Gentamisin ve seftiofur’un lipozomal formülasyonlarının hazırlanması amacıyla Bangham Metodu tercih edildi. Bulgular: Sonuç olarak; seftiofur-1 lipozomunun partikül boyutu 3934.67 nm, polidispersite indeksi 0.471, zeta potansiyeli -10.3 mV'dir; Ceftiofur-2 lipozomunda; parçacık boyutu 4573.00 nm, polidispersite indeksi 0.308, zeta potansiyeli -09.9 mV; Ceftiofur-3 lipozomlarında; parçacık boyutu 479.40 nm, polidispersite indeksi 0.437, zeta potansiyeli -44.8 mV ölçülmüştür. Gentamisin-1 lipozomunda; parçacık boyutu 5185.67 nm, polidispersite indeksi 0.599, zeta potansiyeli -06.5 mV; Gentamisin-2 lipozomunda; parçacık boyutu 4228.00 nm, polidispersite indeksi 0.599, zeta potansiyeli -07.8 mV; Gentamisin-3 lipozomunda; parçacık boyutu 2138.67 nm, polidispersite indeksi 0.565, zeta potansiyeli -06.5 mV ölçülmüştür. Sonuç: Seftiofur ve gentamisinin başarılı bir şekilde lipozomal formülasyonları hazırlandı ve kalite kontrol çalışmaları gerçekleştirildi. Lipozomal formülasyonların etkinliklerinin değerlendirilmesi için ise farmakokinetik/farmakodinamik çalışmaların yapılması gerektiği kanaatine varıldı.
Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
  • Al-Darraji, A., Donahue, R., Tripathi, H., Peng, H., Levitan, B., Chelvarajan, L., Abdel-Latif, A. (2020). Liposomal delivery of azithromycin enhances its immunotherapeutic efficacy and reduces toxicity in myocardial infarction. Scientific Reports, 10. https://doi.org/0.1038/s41598-020-73593-0.
  • Ali, B. H., Al Za'abi, M., Blunden, G., & Nemmar, A. (2011). Experimental gentamicin nephrotoxicity and agents that modify it: a mini-review of recent research. Basic & Clinical Pharmacology & Toxicology, 109(4), 225-232. https://doi.org/10.1111/j.17427843.2011.00728.x.
  • Bangham, A. D., Standish, M. M., & Watkins, J. C. (1965). Diffusion of univalent ions across the lamellae of swollen phospholipids. Journal of Molecular Biology, 13(1), 238-IN227. https://doi.org/10.1016/S0022-2836(65)80093-6.
  • Beltrán-Gracia, E., López-Camacho, A., Higuera-Ciapara, I., Velázquez-Fernández, J. B., & Vallejo-Cardona, A. A. (2019). Nanomedicine review: clinical developments in liposomal applications. Cancer Nanotechnology, 10(1), 11. https://doi.org/10.1186/s12645-019-0055-y.
  • Cavanagh, K. (2012). Plumb’s Veterinary Drug Handbook. Pocket-7th edition. The Canadian Veterinary Journal, 53(12), 1284.
  • Chou, C. K., Li, Y. C., Chen, S. M., Shih, Y. M., & Lee, J. A. (2015). Chitosan prevents gentamicin-induced nephrotoxicity via a carbonyl stress-dependent pathway. BioMed Research International, 2015, 675714. https://doi.org/10.1155/2015/675714.
  • Çoban, Ö., Barut, B., Yalçın, C. Ö., Özel, A., & Bıyıklıoğlu, Z. (2020). Development and in vitro evaluation of BSA-coated liposomes containing Zn (II) phthalocyanine-containing ferrocene groups for photodynamic therapy of lung cancer. Journal of Organometallic Chemistry, 925, 121469. https://doi.org/10.1016/j.jorganchem.2020.121469.
  • Çoban, Ö., Yıldırım, S., & Bakır, T. (2021). Alpha-lipoic acid and cyanocobalamin co-loaded nanoemulsions: development, characterization, and evaluation of stability. Journal of Pharmaceutical Innovation. https://doi.org/10.1007/s12247-020-09531-4.
  • Gonzalez Gomez, A., Syed, S., Marshall, K., & Hosseinidoust, Z. (2019). Liposomal nanovesicles for efficient encapsulation of staphylococcal antibiotics. ACS Omega, 4(6), 10866-10876. doi:10.1021/acsomega.9b00825.
  • Grazia Calvagno, M. C., Christian; Paolino, Donatella; Cosco, Donato; Iannone, Michelangelo; Castelli, Francesco; Doldo, Patrizia; Fresta, Massimo;. (2007). Effects of lipid composition and preparation conditions on physical-chemical properties, technological parameters and in vitro biological activity of gemcitabine-loaded liposomes. Current Drug Delivery, 4(1), 89-101(113).
  • Gupta, A., Mumtaz, S., Li, C.-H., Hussain, I., & Rotello, V. M. (2019). Combatting antibiotic-resistant bacteria using nanomaterials. Chemical Society Reviews, 48(2), 415-427. https://doi.org/10.1039/C7CS00748E.
  • Hornish, R. E., & Kotarski, S. F. (2002). Cephalosporins in veterinary medicine - ceftiofur use in food animals. Current Topics in Medicinal Chemistry, 2(7), 717-731. https://doi.org/10.2174/1568026023393679.
  • Kayaalp, O. (2018). Aminoglikozidler. In O. Kayaalp (Ed.), Akılcıl Tedavi Yönünden Tıbbi Farmakoloji (pp. 233–241). Ankara: Pelikan Yayıncılık.
  • Kim, E. M., & Jeong, H. J. (2021). Liposomes: biomedical applications. Chonnam Medical J., 57(1), 27-35. https://doi.org/10.4068/cmj.2021.57.1.27.
  • Lasic, D. D. (1998). Novel applications of liposomes. Trends in Biotechnology, 16(7), 307-321. doi:10.1016/s0167-7799(98)01220-7.
  • Liu, S., Guo, D., Guo, Y., & Zhou, W. (2011). Preparation and pharmacokinetics of ceftiofur sodium liposomes in cows. Journal of Veterinary Pharmacology and Therapeutics, 34(1), 35-41. https://doi.org/10.1111/j.1365-2885.2010.01190.x.
  • Mugabe, C., Azghani, A. O., & Omri, A. (2005). Liposome-mediated gentamicin delivery: development and activity against resistant strains of Pseudomonas aeruginosa isolated from cystic fibrosis patients. Journal of Antimicrobial Chemotherapy, 55(2), 269-271. doi:10.1093/jac/ dkh518.
  • Pattni, B. S., Chupin, V. V., & Torchilin, V. P. (2015). New developments in liposomal drug delivery. Chemical Reviews, 115(19), 10938-10966. https://doi.org/10.1021/acs.chemrev.5b00046.
  • Saddiqi, M. E., Kadir, A. A., Abdullah, F. F. J., Zakaria, M. Z. A. B., & Banke, I. S. (2022). Preparation, characterization and in vitro cytotoxicity evaluation of free and liposome-encapsulated tylosin. OpenNano, 100108. https://doi.org/10.1016/ j.onano.2022.100108.
  • Shen, S., Wu, Y., Liu, Y., & Wu, D. (2017). High drug-loading nanomedicines: progress, current status, and prospects. International Journal of Nanomedicine, 12, 4085-4109. doi:10.2147/ijn.S132780.
  • Vilos, C., Constandil, L., Rodas, P. I., Cantin, M., Zepeda, K., Herrera, N., & Velasquez, L. A. (2014). Evaluation of ceftiofur-PHBV microparticles in rats. Drug Design, Development and Therapy, 8, 651-666. https://doi.org/10.2147/dddt.S60444.
  • Wagner, K., Vorauer-Uhl, K., & Katinger, H. (2006). Nebulization of liposomal rhcu/zn- Sod with a novel vibrating membrane nebulizer. Journal of Liposome Research, 16, 113–125. https://doi.org/10.1080/08982100600 680733.
APA SUSAR H, ÇELEBİ M, ÇELEBİ Ç, ÇOBAN Ö, ŞEN H, KARAHAN I (2023). The Preparation of Liposomal Formulations of Gentamicin and Ceftiofur Used in Veterinary Medicine. , 554 - 559. 10.53424/balikesirsbd.1262051
Chicago SUSAR Hasan,ÇELEBİ Murat,ÇELEBİ Çağla,ÇOBAN Özlem,ŞEN Hüseyin,KARAHAN IZZET The Preparation of Liposomal Formulations of Gentamicin and Ceftiofur Used in Veterinary Medicine. (2023): 554 - 559. 10.53424/balikesirsbd.1262051
MLA SUSAR Hasan,ÇELEBİ Murat,ÇELEBİ Çağla,ÇOBAN Özlem,ŞEN Hüseyin,KARAHAN IZZET The Preparation of Liposomal Formulations of Gentamicin and Ceftiofur Used in Veterinary Medicine. , 2023, ss.554 - 559. 10.53424/balikesirsbd.1262051
AMA SUSAR H,ÇELEBİ M,ÇELEBİ Ç,ÇOBAN Ö,ŞEN H,KARAHAN I The Preparation of Liposomal Formulations of Gentamicin and Ceftiofur Used in Veterinary Medicine. . 2023; 554 - 559. 10.53424/balikesirsbd.1262051
Vancouver SUSAR H,ÇELEBİ M,ÇELEBİ Ç,ÇOBAN Ö,ŞEN H,KARAHAN I The Preparation of Liposomal Formulations of Gentamicin and Ceftiofur Used in Veterinary Medicine. . 2023; 554 - 559. 10.53424/balikesirsbd.1262051
IEEE SUSAR H,ÇELEBİ M,ÇELEBİ Ç,ÇOBAN Ö,ŞEN H,KARAHAN I "The Preparation of Liposomal Formulations of Gentamicin and Ceftiofur Used in Veterinary Medicine." , ss.554 - 559, 2023. 10.53424/balikesirsbd.1262051
ISNAD SUSAR, Hasan vd. "The Preparation of Liposomal Formulations of Gentamicin and Ceftiofur Used in Veterinary Medicine". (2023), 554-559. https://doi.org/10.53424/balikesirsbd.1262051
APA SUSAR H, ÇELEBİ M, ÇELEBİ Ç, ÇOBAN Ö, ŞEN H, KARAHAN I (2023). The Preparation of Liposomal Formulations of Gentamicin and Ceftiofur Used in Veterinary Medicine. Balıkesir Sağlık Bilimleri Dergisi (BSBD), 12(3), 554 - 559. 10.53424/balikesirsbd.1262051
Chicago SUSAR Hasan,ÇELEBİ Murat,ÇELEBİ Çağla,ÇOBAN Özlem,ŞEN Hüseyin,KARAHAN IZZET The Preparation of Liposomal Formulations of Gentamicin and Ceftiofur Used in Veterinary Medicine. Balıkesir Sağlık Bilimleri Dergisi (BSBD) 12, no.3 (2023): 554 - 559. 10.53424/balikesirsbd.1262051
MLA SUSAR Hasan,ÇELEBİ Murat,ÇELEBİ Çağla,ÇOBAN Özlem,ŞEN Hüseyin,KARAHAN IZZET The Preparation of Liposomal Formulations of Gentamicin and Ceftiofur Used in Veterinary Medicine. Balıkesir Sağlık Bilimleri Dergisi (BSBD), vol.12, no.3, 2023, ss.554 - 559. 10.53424/balikesirsbd.1262051
AMA SUSAR H,ÇELEBİ M,ÇELEBİ Ç,ÇOBAN Ö,ŞEN H,KARAHAN I The Preparation of Liposomal Formulations of Gentamicin and Ceftiofur Used in Veterinary Medicine. Balıkesir Sağlık Bilimleri Dergisi (BSBD). 2023; 12(3): 554 - 559. 10.53424/balikesirsbd.1262051
Vancouver SUSAR H,ÇELEBİ M,ÇELEBİ Ç,ÇOBAN Ö,ŞEN H,KARAHAN I The Preparation of Liposomal Formulations of Gentamicin and Ceftiofur Used in Veterinary Medicine. Balıkesir Sağlık Bilimleri Dergisi (BSBD). 2023; 12(3): 554 - 559. 10.53424/balikesirsbd.1262051
IEEE SUSAR H,ÇELEBİ M,ÇELEBİ Ç,ÇOBAN Ö,ŞEN H,KARAHAN I "The Preparation of Liposomal Formulations of Gentamicin and Ceftiofur Used in Veterinary Medicine." Balıkesir Sağlık Bilimleri Dergisi (BSBD), 12, ss.554 - 559, 2023. 10.53424/balikesirsbd.1262051
ISNAD SUSAR, Hasan vd. "The Preparation of Liposomal Formulations of Gentamicin and Ceftiofur Used in Veterinary Medicine". Balıkesir Sağlık Bilimleri Dergisi (BSBD) 12/3 (2023), 554-559. https://doi.org/10.53424/balikesirsbd.1262051
Sayfa Oluşturulma Tarihi
29-04-2024 12:54

İLETİŞİM

TÜBİTAK ULAKBİM TR Dizin

Yüzüncüyıl, İşçi Blokları Mahallesi

Muhsin Yazıcıoğlu Caddesi No:51/C

06530 Çankaya / ANKARA +90 (312) 298 92 00

trdizin@tubitak.gov.tr

TÜBİTAK ULAKBİM Ulusal Akademik Ağ ve Bilgi Merkezi Cahit Arf Bilgi Merkezi © Tüm Hakları Saklıdır. Gizlilik Politikası Kullanım Şartları