Determination of Amino acid Profile and Some Characteristics of Collagen Extracted from Skin and Bone of Luciobarbus esocinus (Heckel, 1843)
Yıl: 2023 Cilt: 9 Sayı: 2 Sayfa Aralığı: 94 - 107 Metin Dili: İngilizce DOI: 10.17216/limnofish.1213720 İndeks Tarihi: 09-10-2023
Determination of Amino acid Profile and Some Characteristics of Collagen Extracted from Skin and Bone of Luciobarbus esocinus (Heckel, 1843)
Öz: Acid soluble collagens (ASC) from bone (ASC-B) and skin (ASC-S) of mangar (Luciobarbus esocinus (Heckel, 1843) were extracted, characterized, and their amino acid profiles were determined. To best of our knowledge, the current study is the first research that used this species as a source of collagen. Both ASC-S and ASC-B from mangar skin and bone contained glycine as the major amino acid and high amounts of proline, hydroxyproline, alanine, and glutamic acid. On the basis of dry weight, yields of ASC-S and ASC-B were 9.38 and 3.71%, respectively. Furthermore, fourier transform infrared spectroscopy proved that both collagens were integrated and native. Additionally, the results of XRD demonstrated that both of the collagens reserved their helical structures. The screened collagens had prominent absorptions at 230 nm by UV-Vis spectra. Additionally, the SEM studies have shown that both ACS-S and ASC-B have porous and fibrous nature. According to the UV–Vis and FTIR results, extracted collagens were characterized as type I collagen based on their amino acid composition. According to the obtained results, the collagen isolated from mangar can potentially be an alternative source of vertebrate collagens for use in the diet and other industries such as medical and pharmaceutical industries.
Anahtar Kelime: Ferkenin (Luciobarbus esocinus Heckel, 1843) Deri ve Kemiklerinden Ekstrakte Edilen Kollajenin Amino Asit Profili ve Bazı Özelliklerinin Belirlenmesi
Öz: Ferkenin (Luciobarbus esocinus Heckel, 1843) kemiğinden ve derisinden asitte çözünür kolajenler (ASC) ekstrakte edildi, karakterize edildi ve amino asit profilleri belirlendi. Bildiğimiz kadarıyla, yapılan bu çalışma bahsi geçen türü kolajen kaynağı olarak kullanan ilk araştırma olma niteliği taşımaktadır. Ferkenin derisi ve kemiğinden elde edilen ASC’lerin içerisinde ana amino asit olarak glisin ve yüksek miktarlarda prolin, hidroksiprolin, alanin ve glutamik asit içerdiği belirlendi. Kuru ağırlık, ASC-deri ve ASC-kemik verimleri sırasıyla %9,38 ve %3,71 olarak belirlendi. Ayrıca, fourier dönüşümü kızılötesi spektroskopisi (FTIR) ile her iki kolajenin de entegre ve doğal olduğu kanıtlandı. Ek olarak, X-Işını Kırınımı (XRD) sonuçları şunu gösterdi: her iki kolajen sarmal yapılarını korumuştur. Araştırılan kolajenler UV-Vis spektrumları tarafından 230 nm'de belirgin absorpsiyonlara sahiptir. Ayrıca, SEM çalışmaları hem ACS-deri hem de ASC-kemiğin gözenekli ve lifli yapıya sahip olduğunu göstermiştir. UV–Vis ve FTIR sonuçlarına ve ekstrakte edilen kolajenler, amino asit bileşimlerine göre tip I kolajen olarak karakterize edildi. Bu çalışma ile, ferkeden izole edilen kolajenin, diyet, tıp ve ilaç endüstrileri gibi diğer endüstrilerde kullanım için omurgalı kolajenine göre potansiyel olarak alternatif bir kaynak olabileceği belirlenmiştir.
Anahtar Kelime: Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
- Aberoumand A. 2012. Comparative study betweeen different methods of collagen extraction from fish and its properties. World Appl Sci Journa. 16(3):316–319.
- Alves AL, Marques ALP, Martins E, Silva TH, Reis RL. 2017. Cosmetic Potential of Marine Fish Skin Collagen. Cosmetics. 4(4):39. doi: 10.3390/cosmetics4040039
- Benjakul S, Thiansilakul Y, Visessanguan W, Roytrakul S, Kishimura H, Prodpran T, Meesane J. 2010. Extraction and characterisation of pepsin-solubilised collagens from the skin of bigeye snapper (Priacanthus tayenus and Priacanthus macracanthus). J Sci Food Agric. 90(1):132–138. doi: 10.1002/jsfa.3795
- Bigi A, Cojazzi G, Panzavolta S, Rubini K, Roveri N. 2001. Mechanical and thermal properties of gelatin films at different degrees of glutaraldehyde crosslinking. Biomaterials. 22(8):763–768. doi: 10.1016/S0142-9612(00)00236-2
- Borumand M, Sibilla S. 2014. Daily consumption of the collagen supplementPure Gold Collagen® reduces visible signs of aging. Clin Interv Aging. 9:1747–1758. doi: 10.2147/CIA.S65939
- Ciarlo AS, Paredi ME, Fraga AN. 1997. Isolation of Soluble Collagen from Hake Skin (Merluccius hubbsi). J Aquat Food Prod Technol. 6(1):65– 77. doi: 10.1300/J030v06n01_06
- Dave D, Liu Y, Clark L, Dave N, Trenholm S, Westcott J. 2019. Availability of marine collagen from Newfoundland fisheries and aquaculture waste resources. Bioresour Technol Rep. 7:100271. doi: 10.1016/j.biteb.2019.100271
- Doğdu S, Turan C, Ayas D. 2019. Isolation and Characterization of Collagen and Gelatin From Skin of Silver Cheeked Pufferfish Lagocephalus sceleratus for Pharmaceutical and Biomedical Applications. NESciences. doi: 10.28978/nesciences.661099
- Doyle BB, Bendit EG, Blout ER. 1975. Infrared spectroscopy of collagen and collagen-like polypeptides. Biopolymers. 14(5):937–957. doi: 10.1002/bip.1975.360140505
- Duan R, Zhang J, Du X, Yao X, Konno K. 2009. Properties of collagen from skin, scale and bone of carp (Cyprinus carpio). Food Chem. 112(3):702–706. doi: 10.1016/j.foodchem.2008.06.020
- EFSA Panel on Biological Hazards (BIOHAZ), Koutsoumanis K, Allende A, Bolton DJ, Bover-Cid S, Chemaly M, Davies R, De Cesare A, Herman LM, Hilbert F, et al. 2020. Potential BSE risk posed by the use of ruminant collagen and gelatine in feed for non-ruminant farmed animals. EFSA J Eur Food Saf Auth. 18(10):e06267. doi: 10.2903/j.efsa.2020.6267
- Freyhof J. 2014. Luciobarbus esocinus Heckel, 1843. [accessed 2021 Nov 12]. Available from https://www.gbif.org/species/176724980
- Fricke R, Bilecenoglu M, Sarı H. 2007. Annotated Checklist of Fish and Lamprey Species (Gnathostomata and Petromyzontomorphi) of Turkey, Including a Red List of Threatened and Declining Species. Stuttg Beitr Zur Naturkunde A. 706:1–169.
- Gelse K, Pöschl E, Aigner T. 2003. Collagens-structure, function, and biosynthesis. Adv Drug Deliv Rev. 55(12):1531–1546. doi: 10.1016/j.addr.2003.08.002
- Göçer M. 2022. Extraction and characterization of collagen from the skin and bone of shabout (Arabibarbus grypus Heckel, 1843). Iran J Fish Sci. 21(3):671–687. doi: 10.22092/ijfs.2022.126898
- Grossman S, Bergman M. 1992. Process for the production of gelatin from fish skins; [accessed 2021 Nov 12]. Available from https://patents.google.com/patent/US5093474 A/en
- Hsieh C-H, Shiau C-Y, Su Y-C, Liu Y-H, Huang Y-R. 2016. Isolation and characterization of collagens from the skin of giant grouper (Epinephelus lanceolatus). J Aquat Food Prod Technol. 25(1):93–104. doi: 10.1080/10498850.2013.828145
- Huo J, Zhao Z. 2009. Study on Enzymatic Hydrolysis of Gadus morrhua Skin Collagen and Molecular Weight Distribution of Hydrolysates. Agric Sci China. 8(6):723–729. doi: 10.1016/S1671-2927(08)60271-0
- Jaziri AA, Shapawi R, Mokhtar RAM, Noordin WNM, Huda N. 2022. Microstructural and Physicochemical Analysis of Collagens from the Skin of Lizardfish (Saurida tumbil Bloch, 1795) Extracted with Different Organic Acids. Mol Basel Switz. 27(8):2452. doi: 10.3390/molecules27082452
- Jongjareonrak A, Benjakul S, Visessanguan W, Nagai T, Tanaka M. 2005. Isolation and characterisation of acid and pepsin-solubilised collagens from the skin of Brownstripe red snapper (Lutjanus vitta). Food Chem. 93(3):475–484. doi: 10.1016/j.foodchem.2004.10.026
- Kiew PL, Mat Don M. 2012. Collagen Extraction from Malaysian Cultured Catfish (Hybrid Clarias Sp.): Kinetics and Optimization of Extraction Conditions Using Response Surface Methodology. ISRN Chem Eng. 2012:e835391. doi: 10.5402/2012/835391
- Kittiphattanabawon P, Benjakul S, Visessanguan W, Nagai T, Tanaka M. 2005. Characterisation of acid-soluble collagenfrom skin and bone of bigeye snapper (Priacanthus tayenus).Food Chem. 89(3):363–372. doi: 10.1016/j.foodchem.2004.02.042
- Komsa-Penkova R, Koynova R, Kostov G, Tenchov B. 2000. Discrete reduction of type I collagen thermal stability upon oxidation. Biophys Chem. 83(3):185–195. doi: 10.1016/S0301-4622(99)00135-0
- Krimm S, Bandekar J. 1986. Vibrational Spectroscopy and Conformation of Peptides, Polypeptides, and Proteins. In: Anfinsen CB, Edsall JT, Richards FM, editors. Adv Protein Chem. Vol. 38. Academic Press; [accessed 2021 Nov 12]; p. 181–364. doi: 10.1016/S0065-3233(08)60528-8
- Kumar B, Rani S. 2017. Technical note on the isolation and characterization of collagen from fish waste material. J Food Sci Technol. 54(1):276– 278. doi: 10.1007/s13197-016-2443-1
- Mahboob S, Al-Ghanim KA, Sultana S, Al-Balawi HFA, Sultana T, Ashraf A, Al-Misned F, Ahmed Z. 2015. Assessment of Meat Quality and Dressing Losses in Wild and Farmed. Pak J Zool. 47(6):1753–1759.
- Matmaroh K, Benjakul S, Prodpran T, Encarnacion AB, Kishimura H. 2011. Characteristics of acid soluble collagen and pepsin soluble collagen from scale of spotted golden goatfish (Parupeneus heptacanthus). Food Chem. 129(3):1179–1186. doi: 10.1016/j.foodchem.2011.05.099
- Mørkøre T, Moreno H, Borderías AJ, Larsson T, Hellberg H, Hatlen B, Romarheim O, Ruyter B, Lazado C, Jiménez-Guerrero R, et al. 2020. Dietary inclusion of Antarctic krill meal during the finishing feed period improves health and fillet quality of Atlantic salmon ( Salmo salar L.). Br J Nutr. 124:1–40. doi: 10.1017/S0007114520001282
- Muyonga JH, Cole CGB, Duodu KG. 2004. Characterisation of acid soluble collagen from skins of young and adult Nile perch (Lates niloticus). Food Chem. 85(1):81–89. doi: 10.1016/j.foodchem.2003.06.006
- Nagai T, Suzuki N. 2000. Preparation and Characterization of Several Fish Bone Collagens. J Food Biochem. 24(5):427–436. doi: 10.1111/j.1745-4514.2000.tb00711.x
- Nathanson N, Wilesmith J, Griot C. 1997. Bovine spongiform encephalopathy (BSE): causes and consequences of a common source epidemic. Am J Epidemiol. 145(11):959–969. doi: 10.1093/oxfordjournals.aje.a009064
- Noorzai S, Verbeek C. 2020. Characterization of acid- and pepsin-soluble collagen extracted from the skin of Nile tilapia (Oreochromis niloticus) - ScienceDirect. In: Biotechnol Appl Biomass. Intechopen; [accessed 2021 Nov 12]. Available from https://www.sciencedirect.com/science/article/abs/pii/S0141813016326423
- Ozgur M. 2016. The Luciobarbus esocinus (Heckel, 1843) From The Euphrates Rıver Basın: An Introductıon About Its Past, Present And Future. Paper presented at: 2nd International Congress on Applied chthyology & Aquatic Environment, Messolonghi, Greece.
- Pallant J. 2020. SPSS Survival Manual: A Step by Step Guide to Data Analysis Using IBM SPSS. 7th ed. London: Routledge. doi: 10.4324/9781003117452
- Potaros T, Raksakulthai N, Runglerdkreangkrai J, Worawattanamateekul W. 2009. Characteristics of collagen from Nile tilapia (Oreochromis niloticus) skin isolated by two different methods. Nat Sci. 43:584–593.
- Reátegui-Pinedo N, Salirrosas D, Sánchez-Tuesta L, Quiñones C, Jáuregui-Rosas SR, Barraza G, Cabrera A, Ayala-Jara C, Martinez RM, Baby AR, Prieto ZA. 2022. Characterization of Collagen from Three Genetic Lines (Gray, Red and F1) of Oreochromis niloticus (Tilapia) Skin in Young and Old Adults. Molecules. 27(3):1123. doi: 10.3390/molecules27031123
- Sadowska M, Kołodziejska I, Niecikowska C. 2003. Isolation of collagen from the skins of Baltic cod (Gadus morhua). Food Chem. 81(2):257–262. doi: 10.1016/S0308-8146(02)00420-X
- Sai KP, Babu M. 2001. Studies on Rana tigerina skin collagen. Comp Biochem Physiol BBiochem Mol Biol. 128(1):81–90. doi: 10.1016/s1096-4959(00)00301-8
- Salvatore L, Gallo N, Natali ML, Terzi A, Sannino A, Madaghiele M. 2021. Mimicking the Hierarchical Organization of Natural Collagen: Toward the Development of Ideal Scaffolding Material for Tissue Regeneration. Front Bioeng Biotechnol. 9:644595. doi: 10.3389/fbioe.2021.644595
- Shalaby M, Agwa M, Saeed H, Khedr SM, Morsy O, El-Demellawy MA. 2020. Fish Scale Collagen Preparation, Characterization and Its Application in Wound Healing. J Polym Environ. 28(1):166–178. doi: 10.1007/s10924-019-01594-w
- Sherratt MJ. 2009. Tissue elasticity and the ageing elastic fibre. Age Dordr Neth. 31(4):305–325. doi: 10.1007/s11357-009-9103-6
- Singh P, Benjakul S, Maqsood S, Kishimura H. 2011. Isolation and characterisation of collagen extracted from the skin of striped catfish (Pangasianodon hypophthalmus). Food Chem. 124(1):97–105. doi: 10.1016/j.foodchem.2010.05.111
- Sionkowska A, Adamiak K, Musiał K, Gadomska M. 2020. Collagen Based Materials in Cosmetic Applications: A Review. Mater Basel Switz. 13(19):E4217. doi: 10.3390/ma13194217
- Sun L, Li B, Song W, Si L, Hou H. 2017. Characterization of Pacific cod (Gadus macrocephalus) skin collagen and fabrication of collagen sponge as a good biocompatible biomedical material. Process Biochem. 63:229–235. doi: 10.1016/j.procbio.2017.08.003
- Tamilmozhi S, Veeruraj A, Arumugam M. 2013. Isolation and characterization of acid and pepsin-solubilized collagen from the skin of sailfish (Istiophorus platypterus). Food Res Int. 54(2):1499–1505. doi: 10.1016/j.foodres.2013.10.002
- Tang L, Chen S, Su W, Weng W, Osako K, Tanaka M. 2015. Physicochemical properties and film-forming ability of fish skin collagen extracted from different freshwater species. Process Biochem. 50(1):148–155. doi: 10.1016/j.procbio.2014.10.015
- Wang L, Liang Q, Chen T, Wang Z, Xu J, Ma H. 2014. Characterization of collagen from the skin of Amur sturgeon (Acipenser schrenckii). Food Hydrocoll. 38:104–109. doi: 10.1016/j.foodhyd.2013.12.002
- Wang S, Hou H, Hou J, Tao Y, Lu Y, Yang X, Li B. 2013. Characterization of Acid-Soluble Collagen From Bone of Pacific Cod (Gadus macrocephalus). J Aquat Food Prod Technol. 22(4):407–420. doi: 10.1080/10498850.2011.654382
- Wei P, Zheng H, Shi Z, Li D, Xiang Y. 2019. Isolation and Characterization of Acid-soluble Collagen and Pepsin-soluble Collagen from the Skin of Hybrid Sturgeon. J Wuhan Univ Technol-Mater Sci Ed. 34(4): 950–959. doi: 10.1007/s11595-019-2143-6
- Yata M, Yoshida C, Fujisawa S, Mizuta S, Yoshinaka R. 2001. Identification and Characterization of Molecular Species of Collagen in Fish Skin. J. Food Sci. doi:10.1111/J.1365-2621.2001.TB11325.X
- Zhang F, Wang A, Li Z, He S, Shao L. 2011. Preparation and Characterisation of Collagen from Freshwater Fish Scales. Food Nutr Sci. 2(8):818–823. doi: 10.4236/fns.2011.28112
- Zhang Y, Liu W, Li G, Shi B, Miao Y, Wu X. 2007. Isolation and partial characterization of pepsin-soluble collagen from the skin of grass carp (Ctenopharyngodon idella). Food Chem. 103(3):906–912. doi: 10.1016/j.foodchem.2006.09.053
| APA | GOÇER M, YANAR Y, aydin m (2023). Determination of Amino acid Profile and Some Characteristics of Collagen Extracted from Skin and Bone of Luciobarbus esocinus (Heckel, 1843). , 94 - 107. 10.17216/limnofish.1213720 |
| Chicago | GOÇER MUSTAFA,YANAR YASEMEN,aydin muhsin Determination of Amino acid Profile and Some Characteristics of Collagen Extracted from Skin and Bone of Luciobarbus esocinus (Heckel, 1843). (2023): 94 - 107. 10.17216/limnofish.1213720 |
| MLA | GOÇER MUSTAFA,YANAR YASEMEN,aydin muhsin Determination of Amino acid Profile and Some Characteristics of Collagen Extracted from Skin and Bone of Luciobarbus esocinus (Heckel, 1843). , 2023, ss.94 - 107. 10.17216/limnofish.1213720 |
| AMA | GOÇER M,YANAR Y,aydin m Determination of Amino acid Profile and Some Characteristics of Collagen Extracted from Skin and Bone of Luciobarbus esocinus (Heckel, 1843). . 2023; 94 - 107. 10.17216/limnofish.1213720 |
| Vancouver | GOÇER M,YANAR Y,aydin m Determination of Amino acid Profile and Some Characteristics of Collagen Extracted from Skin and Bone of Luciobarbus esocinus (Heckel, 1843). . 2023; 94 - 107. 10.17216/limnofish.1213720 |
| IEEE | GOÇER M,YANAR Y,aydin m "Determination of Amino acid Profile and Some Characteristics of Collagen Extracted from Skin and Bone of Luciobarbus esocinus (Heckel, 1843)." , ss.94 - 107, 2023. 10.17216/limnofish.1213720 |
| ISNAD | GOÇER, MUSTAFA vd. "Determination of Amino acid Profile and Some Characteristics of Collagen Extracted from Skin and Bone of Luciobarbus esocinus (Heckel, 1843)". (2023), 94-107. https://doi.org/10.17216/limnofish.1213720 |
| APA | GOÇER M, YANAR Y, aydin m (2023). Determination of Amino acid Profile and Some Characteristics of Collagen Extracted from Skin and Bone of Luciobarbus esocinus (Heckel, 1843). Journal of Limnology and Freshwater Fisheries Research, 9(2), 94 - 107. 10.17216/limnofish.1213720 |
| Chicago | GOÇER MUSTAFA,YANAR YASEMEN,aydin muhsin Determination of Amino acid Profile and Some Characteristics of Collagen Extracted from Skin and Bone of Luciobarbus esocinus (Heckel, 1843). Journal of Limnology and Freshwater Fisheries Research 9, no.2 (2023): 94 - 107. 10.17216/limnofish.1213720 |
| MLA | GOÇER MUSTAFA,YANAR YASEMEN,aydin muhsin Determination of Amino acid Profile and Some Characteristics of Collagen Extracted from Skin and Bone of Luciobarbus esocinus (Heckel, 1843). Journal of Limnology and Freshwater Fisheries Research, vol.9, no.2, 2023, ss.94 - 107. 10.17216/limnofish.1213720 |
| AMA | GOÇER M,YANAR Y,aydin m Determination of Amino acid Profile and Some Characteristics of Collagen Extracted from Skin and Bone of Luciobarbus esocinus (Heckel, 1843). Journal of Limnology and Freshwater Fisheries Research. 2023; 9(2): 94 - 107. 10.17216/limnofish.1213720 |
| Vancouver | GOÇER M,YANAR Y,aydin m Determination of Amino acid Profile and Some Characteristics of Collagen Extracted from Skin and Bone of Luciobarbus esocinus (Heckel, 1843). Journal of Limnology and Freshwater Fisheries Research. 2023; 9(2): 94 - 107. 10.17216/limnofish.1213720 |
| IEEE | GOÇER M,YANAR Y,aydin m "Determination of Amino acid Profile and Some Characteristics of Collagen Extracted from Skin and Bone of Luciobarbus esocinus (Heckel, 1843)." Journal of Limnology and Freshwater Fisheries Research, 9, ss.94 - 107, 2023. 10.17216/limnofish.1213720 |
| ISNAD | GOÇER, MUSTAFA vd. "Determination of Amino acid Profile and Some Characteristics of Collagen Extracted from Skin and Bone of Luciobarbus esocinus (Heckel, 1843)". Journal of Limnology and Freshwater Fisheries Research 9/2 (2023), 94-107. https://doi.org/10.17216/limnofish.1213720 |
