TY  - JOUR
TI  - Comparıson of cumulus cells and follıcular fluıd obtaıned from ınfertile ındıvıduals dıagnosed wıth polycystıc ovary syndrome (PCOS) and endomethrıosıs wıth samples obtaıned from healthy ındıvıduals
AB  - Aim: Investigating the relationship between Growth differentiation factor-9 (GDF-9), Bone morphogenetic protein-15 (BMP-15) markers, apoptosis levels in cumulus cells and total oxidant (TOS)/ anti-oxidant (TAS) stress levels,  inflammation parameters (interleukin-6 (IL-6), tumor necrosis factor alpha (TNF-alpha)) in follicular fluid belonging to patients with polycystic ovary syndrome (PCOS), endometriosis (END) and male factor (MF) (control)  groups.  Material and Methods: GDF-9 and BMP-15 markers are determined by immunohistochemical methods, apoptosis levels are studied  with TUNEL. TOS and TAS statuses are investigated with spectrophotometry, IL-6 and TNF – alpha levels are examined by Enzyme-Linked Immuno Sorbent Assay (ELISA).  Results: According to the data obtained in the study; GDF-9 and BMP-15 levels are found to be lower in PCOS and END groups and  apoptosis levels of cumulus cells were significantly higher at these groups . TOS levels were significantly higher in PCOS and END groups whereas follicular fluid TAS levels were not statistically significant for these groups. IL-6 and TNF – alpha levels of follicular fluid was significantly higher in PCOS. These parameters were also higher for END group, however the difference was not found to be significant. Conclusion: Our results imply that correlation between GDF-9, BMP-15 markers, apotosis levels, oxidative status, inflammation levels may be interpreted with improper environment for oocyte maturation for patients diagnosed with PCOS or END.  The need for further studies on  subject proceeds. However, if similar datas are obtained in further studies, it is thought that  evaluation of cumulus cell properties together with especially follicular fluid oxidative stress levels will contribute to the selection of the best oocyte.
AU  - Gürgan, Timur
AU  - Ruso, Halil
AU  - TURAL, Rabia
AU  - Sepici Dincel, Aylin
AU  - DAYANIR, Duygu
AU  - Kalem, Ziya
AU  - Saribas, Gulistan Sanem
AU  - Özoğul, Candan
DO  - 10.18663/tjcl.1339043
PY  - 2023
JO  - Turkish Journal of Clinics and Laboratory
VL  - 14
IS  - 3
SN  - 2149-8296
SP  - 576
EP  - 586
DB  - TRDizin
UR  - http://search/yayin/detay/1200423
ER  -