TY  - JOUR
TI  - Antiproliferative Properties and Evaluation of Antioxidant of Different Cornelian Cherry Genotypes and Analysis of Phenolic and Sugar Compounds by HPLC
AB  - The aim of this study was to investigate the chemical composition (vitamin C, phenolic, and sugar compounds), the cyto -toxic effect on healthy (L-929) and lung cancer (A-549) cells, and the antioxidant capacity of fruits belong to thirteen cornelian cherry (Cornus mas L.) genotypes grown under the same conditions in Turkey. Fruit samples were extracted by the ASE technique. The chemical composition was analyzed by HPLC-DAD-RID. A reversed-phase Clipeus C18 reversed-pha - se column (250 mm × 4.6 mm, 5 μm) were used. For gradient elution mobile phase A contained 4.5 % acetic acid in water; solution B acetonitrile were used as mobile phase with flow rate 1.0 mL/min. Antioxidant capacity, total phenolic, and total anthocyanin content were determined using spectrophotometric methods. Cytotoxic effects were evaluated by MTT assay in L-929 and A-549 cell lines for 48 h. No toxic effect of the fruit extracts was observed on L-929 healthy mouse fibroblast cells, while it was determined to reduce cell proliferation (approximately 50%) on A-549 lung cancer cells. The featured genotypes were 44-03, 44-20, 44-21, 77-09, and 44-21, 44-16, 77-05, respectively. The featured genotypes for antioxidant capacity and cytotoxic effects on A-549 cells were 44-03, 44-20, 44-21, 77-09, and 44-21, 44-16, 77-05, respectively. The results have brought out that there are significant differences between the genotypes (p ≤ 0.05) and cornelian cherry fruits have a significant antioxidant capacity and potential for antiproliferative effects.
AU  - Erdoğan, Selim
AU  - UGUR, Yılmaz
AU  - Salva, Emine
AU  - KARAAT, FIRAT EGE
DO  - 10.15671/hjbc.1065317
PY  - 2023
JO  - Hacettepe Journal of Biology and Chemistry
VL  - 51
IS  - 1
SN  - 1303-5002
SP  - 57
EP  - 70
DB  - TRDizin
UR  - http://search/yayin/detay/1200428
ER  -