Yıl: 2023 Cilt: 51 Sayı: 2 Sayfa Aralığı: 141 - 152 Metin Dili: İngilizce DOI: 10.15671/hjbc.1187546 İndeks Tarihi: 17-10-2023

Enhanced Petroleum Removal by Potent Biosurfactant Producer Bacillus Subtilis CC9 Strain Isolated from an Oil Field

Öz:
This study aimed to enhance the removal of petroleum with potent biosurfactant producing bacterial strains (Bacillius subtilis CC9, Enterobacter asburiae WMB1, Klebsiella oxytoca Rizhao 536-1, and Acinetobacter pittii PgBE252) isolated from an oil field. The biosurfactant production was determined by different screening methods such as hemolytic activity, drop-collapse, oil spreading, BATH, emulsification index and surface tension. While the presence of biosurfactant was detected with individual bacterial strain, B. subtilis CC9 strain was found to be quite effective on petroleum degradation (92%) with a high yield of biosurfactant production (0.95 ±0.05 g L-1). The degradation rate constant and half-life period of B. subtilis CC9 strain were calculated as K = 2.533 day−1, t1/2 = 0.273. It has been clearly shown with GC/MS analysis that B. subtilis CC9 strain was effective on the removal of long chain n-alkanes (C31 - C35) as well as short and medium chains. The FT-IR analysis also approved the presence of aliphatic and peptide moieties of biosurfactant extracted from B. subtilis CC9 strain. It is remarkably pointed out with this study that high yield of biosurfactant producing B. subtilis CC9 strain can be used in advanced petroleum bioremediation studies as an alternative to chemical surfactants.
Anahtar Kelime: Bacillus subtilis biosurfactant petroleum biodegradation

Petrol Sahasından İzole Edilen Güçlü Biyosürfaktan Üreticisi Bacillus Subtilis CC9 Suşu ile Petrol Gideriminin Arttırılması

Öz:
Bu çalışmada, petrol sahasından izole edilen potent biyosürfektan üreticisi bakteriyel suşlar ( Bacillius subtilis CC9, Ente- robacter asburiae WMB1, Klebsiella oxytoca Rizhao 536-1, ve Acinetobacter pittii PgBE252) ile petrol gideriminin arttı - rılması amaçlanmıştır. Hemolitik aktivite, yayılma-daralma, petrol yayılma, BATH, emülsifikasyon indeksi ve yüzey gerilimi gibi farklı tarama yöntemleriyle biyosürfektan üretimi belirlenmiştir. Her bir bakteriyel suşta biyosürfektan varlığı tespit edilirken, B. subtilis CC9 suşunun biyosürfektan üretimi (0.95 ±0.05 g L -1) ile beraber petrol degradasyonu (%92) üzerinde de oldukça etkili olduğu bulunmuştur. B. subtilis CC9 suşunun bozunma hız sabiti K = 2.533 day−1 ve yarılanma ömrü t1/2 = 0.273 olarak hesaplanmıştır. GC/MS analizi ile B. subtilis CC9 suşunun uzun zincirli n-alkanların (C31 - C 35) yanı sıra kısa ve orta zincirli n-alkanların degradasyonunda da etkili olduğu açıkça gösterilmiştir. B. subtilis CC9 suşundan ekstrakte edilen biyosürfektana ait alifatik ve peptit kısımların varlığı FT-IR analizi ile doğrulanmıştır. Bu çalışma ile, yüksek verimle biyosür- fektan üreten B. subtilis CC9 suşunun kimyasal sürfaktanlara alternatif olarak ileri petrol biyoremediasyon çalışmalarında kullanılabileceğine dikkat çekilmiştir.
Anahtar Kelime: Bacillus subtilis biyosürfektan petrol biyoyıkım

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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APA Bilen Ozyurek S, Soyuer K (2023). Enhanced Petroleum Removal by Potent Biosurfactant Producer Bacillus Subtilis CC9 Strain Isolated from an Oil Field. , 141 - 152. 10.15671/hjbc.1187546
Chicago Bilen Ozyurek Sezen,Soyuer Kaan Enhanced Petroleum Removal by Potent Biosurfactant Producer Bacillus Subtilis CC9 Strain Isolated from an Oil Field. (2023): 141 - 152. 10.15671/hjbc.1187546
MLA Bilen Ozyurek Sezen,Soyuer Kaan Enhanced Petroleum Removal by Potent Biosurfactant Producer Bacillus Subtilis CC9 Strain Isolated from an Oil Field. , 2023, ss.141 - 152. 10.15671/hjbc.1187546
AMA Bilen Ozyurek S,Soyuer K Enhanced Petroleum Removal by Potent Biosurfactant Producer Bacillus Subtilis CC9 Strain Isolated from an Oil Field. . 2023; 141 - 152. 10.15671/hjbc.1187546
Vancouver Bilen Ozyurek S,Soyuer K Enhanced Petroleum Removal by Potent Biosurfactant Producer Bacillus Subtilis CC9 Strain Isolated from an Oil Field. . 2023; 141 - 152. 10.15671/hjbc.1187546
IEEE Bilen Ozyurek S,Soyuer K "Enhanced Petroleum Removal by Potent Biosurfactant Producer Bacillus Subtilis CC9 Strain Isolated from an Oil Field." , ss.141 - 152, 2023. 10.15671/hjbc.1187546
ISNAD Bilen Ozyurek, Sezen - Soyuer, Kaan. "Enhanced Petroleum Removal by Potent Biosurfactant Producer Bacillus Subtilis CC9 Strain Isolated from an Oil Field". (2023), 141-152. https://doi.org/10.15671/hjbc.1187546
APA Bilen Ozyurek S, Soyuer K (2023). Enhanced Petroleum Removal by Potent Biosurfactant Producer Bacillus Subtilis CC9 Strain Isolated from an Oil Field. Hacettepe Journal of Biology and Chemistry, 51(2), 141 - 152. 10.15671/hjbc.1187546
Chicago Bilen Ozyurek Sezen,Soyuer Kaan Enhanced Petroleum Removal by Potent Biosurfactant Producer Bacillus Subtilis CC9 Strain Isolated from an Oil Field. Hacettepe Journal of Biology and Chemistry 51, no.2 (2023): 141 - 152. 10.15671/hjbc.1187546
MLA Bilen Ozyurek Sezen,Soyuer Kaan Enhanced Petroleum Removal by Potent Biosurfactant Producer Bacillus Subtilis CC9 Strain Isolated from an Oil Field. Hacettepe Journal of Biology and Chemistry, vol.51, no.2, 2023, ss.141 - 152. 10.15671/hjbc.1187546
AMA Bilen Ozyurek S,Soyuer K Enhanced Petroleum Removal by Potent Biosurfactant Producer Bacillus Subtilis CC9 Strain Isolated from an Oil Field. Hacettepe Journal of Biology and Chemistry. 2023; 51(2): 141 - 152. 10.15671/hjbc.1187546
Vancouver Bilen Ozyurek S,Soyuer K Enhanced Petroleum Removal by Potent Biosurfactant Producer Bacillus Subtilis CC9 Strain Isolated from an Oil Field. Hacettepe Journal of Biology and Chemistry. 2023; 51(2): 141 - 152. 10.15671/hjbc.1187546
IEEE Bilen Ozyurek S,Soyuer K "Enhanced Petroleum Removal by Potent Biosurfactant Producer Bacillus Subtilis CC9 Strain Isolated from an Oil Field." Hacettepe Journal of Biology and Chemistry, 51, ss.141 - 152, 2023. 10.15671/hjbc.1187546
ISNAD Bilen Ozyurek, Sezen - Soyuer, Kaan. "Enhanced Petroleum Removal by Potent Biosurfactant Producer Bacillus Subtilis CC9 Strain Isolated from an Oil Field". Hacettepe Journal of Biology and Chemistry 51/2 (2023), 141-152. https://doi.org/10.15671/hjbc.1187546