Yıl: 2023 Cilt: 51 Sayı: 6 Sayfa Aralığı: 201 - 214 Metin Dili: İngilizce DOI: 10.15671/hjbc.1215813 İndeks Tarihi: 17-10-2023

A Practical and Single-Use ITO-Pet Based Immunosensing Platform for Detection of Tumor Necrosis Factor-Alpha Biomarker

Öz:
This investigation displays a novel, practical indium tin oxide- polyethylene terephthalate (ITO-PET) based electrochemical biosensor for the Tumor Necrosis Factor-alpha (TNFα) biomarker determination. The ITO-PET electrode is a very ad - vantageous preferred semiconductive electrode material. It has a lot of great features such as easy to prepare, cheapness, flexibility, stability. It also allows determining an analyte at very low concentrations and provides a great wide concentration range for analyte analysis. Electrochemical Impedance Spectroscopy (EIS) and Cyclic Voltammetry (CV) were used for the evaluation of biosensors, including the immobilization procedure, the investigation of the optimum conditions, and the characterization of biosensors. The immunosensor's electrode surface morphology during the immobilization process was observed using a Scanning Electron Microscope (SEM). In addition, Impedance measurement at a single frequency was used to characterize anti-TNFα and TNFα interactions (SFI). The clinical effectiveness of the developed biosensor was investiga - ted by testing it with real human serum samples. Moreover, the fabricated immunosensor presents long shelf life, analysis of the antigen concentrations at picogram level (0.02 pg mL-1 -2.56 pg mL-1), reproducibility, reusability and high sensitivity
Anahtar Kelime: TNFα immunosensor ITO-PET electrode electrochemical impedance spectroscopy

Investigation of the effects of angiotensin converting enzyme inhibitors and angiotensin receptor blockers on anemia in patients with normal or mildly low glomerular filtration rate

Öz:
Background/aim: The relationship between the activation of the renin-angiotensin system and the increase in erythropoiesis has been shown in many studies. In addition, the use of angiotensin converting enzyme inhibitors (ACEIs) or angiotensin-receptor blockers (ARB) has been reported to reduce hemoglobin levels in various patient groups at risk for secondary erythrocytosis/polycythemia. The aim of our study is to investigate whether there is a change in hemoglobin levels after starting ACEIs or ARBs in patients who have not used them before. Materials and methods: Three hundred and fifty-one patients who were started on renin angiotensin aldosterone system (RAAS) blockers were evaluated retrospectively. None of the patients had anemia before starting RAAS blockers. A median of 6 (4–12) months after the start of the drug, complete blood count and kidney function tests were evaluated. Hemoglobin values before and after the start of the drug were compared statistically. Results: A statistically significant decrease in mean Hb value was found after starting ACEIs or ARBs (14.39 ± 1.29 g/dL vs 13.98 ± 1.36 g/dL, p < 0.001). The decrease in control Hb values was higher in the ARB group than in the ACEI group (–0.53 ± 0.06 g/dL vs –0.29 ± 0.06 g/dL, p < 0.001). Conclusion: A significant decrease in mean Hb level was detected in the first year following the first administration of ACEIs or ARBs.Key words: Anemia, hypertension, renin, angiotensin, aldosterone
Anahtar Kelime:

Tümör Nekrozis Faktörü-Alfa Biyobelirteç Tayini için Pratik ve Tek Kullanımlık ITO- PET Bazlı İmmunosensör Platformu

Öz:
Bu araştırma, Tümör Nekrozis Faktörü-alfa (TNFa) biyobelirteç tayini için yeni, pratik bir indiyum kalay oksit-polietilen tereftalat (ITO-PET) bazlı elektrokimyasal biyosensörü göstermektedir. ITO-PET elektrodu çok avantajlı olarak tercih edilen bir yarı iletken elektrot malzemesidir. Hazırlanmasının kolay olması, ucuz olması, esnekliği, sağlamlığı gibi bir çok iyi özelliği vardır. Ayrıca çok düşük konsantrasyonlarda bir analitin belirlenmesine izin verir ve analit analizi için çok geniş bir konsantrasyon aralığı sağlar. İmmobilizasyon prosedürü, optimum koşulların araştırılması ve biyosensörlerin karakterizas - yonu dahil olmak üzere biyosensörlerin değerlendirilmesi için Elektrokimyasal Empedans Spektroskopisi (EIS) ve Döngüsel Voltametri (CV) kullanıldı. İmmünosensörün immobilizasyon işlemi sırasında elektrot yüzey morfolojisi bir Taramalı Elektron Mikroskobu (SEM) kullanılarak gözlendi. Ayrıca, anti-TNFα ve TNFα etkileşimlerini karakterize etmek için tek bir frekansta empedans (SFI) ölçümü kullanıldı. Geliştirilen biyosensörün klinik etkinliği, gerçek insan serum numuneleri ile test edilerek araştırıldı. Ayrıca, tasarlanan immünosensör uzun raf ömrü, pikogram düzeyinde antijen konsantrasyonlarının analizi (0.02 pg mL-1 -2.56 pg mL-1), tekrar üretilebilirlik, yeniden kullanılabilirlik ve yüksek hassasiyet sunar.
Anahtar Kelime: TNFα ITO-PET elektrot elektrokimyasal impedans spektroskopisi immunosensör

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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APA Özcan B, ERGÜN B, aybal kutlugun a, ergün m, Aktürk G, Beyan E (2023). A Practical and Single-Use ITO-Pet Based Immunosensing Platform for Detection of Tumor Necrosis Factor-Alpha Biomarker. , 201 - 214. 10.15671/hjbc.1215813
Chicago Özcan Burcu,ERGÜN Betül,aybal kutlugun aysun,ergün mustafa çağrı,Aktürk Galip,Beyan Esin A Practical and Single-Use ITO-Pet Based Immunosensing Platform for Detection of Tumor Necrosis Factor-Alpha Biomarker. (2023): 201 - 214. 10.15671/hjbc.1215813
MLA Özcan Burcu,ERGÜN Betül,aybal kutlugun aysun,ergün mustafa çağrı,Aktürk Galip,Beyan Esin A Practical and Single-Use ITO-Pet Based Immunosensing Platform for Detection of Tumor Necrosis Factor-Alpha Biomarker. , 2023, ss.201 - 214. 10.15671/hjbc.1215813
AMA Özcan B,ERGÜN B,aybal kutlugun a,ergün m,Aktürk G,Beyan E A Practical and Single-Use ITO-Pet Based Immunosensing Platform for Detection of Tumor Necrosis Factor-Alpha Biomarker. . 2023; 201 - 214. 10.15671/hjbc.1215813
Vancouver Özcan B,ERGÜN B,aybal kutlugun a,ergün m,Aktürk G,Beyan E A Practical and Single-Use ITO-Pet Based Immunosensing Platform for Detection of Tumor Necrosis Factor-Alpha Biomarker. . 2023; 201 - 214. 10.15671/hjbc.1215813
IEEE Özcan B,ERGÜN B,aybal kutlugun a,ergün m,Aktürk G,Beyan E "A Practical and Single-Use ITO-Pet Based Immunosensing Platform for Detection of Tumor Necrosis Factor-Alpha Biomarker." , ss.201 - 214, 2023. 10.15671/hjbc.1215813
ISNAD Özcan, Burcu vd. "A Practical and Single-Use ITO-Pet Based Immunosensing Platform for Detection of Tumor Necrosis Factor-Alpha Biomarker". (2023), 201-214. https://doi.org/10.15671/hjbc.1215813
APA Özcan B, ERGÜN B, aybal kutlugun a, ergün m, Aktürk G, Beyan E (2023). A Practical and Single-Use ITO-Pet Based Immunosensing Platform for Detection of Tumor Necrosis Factor-Alpha Biomarker. Hacettepe Journal of Biology and Chemistry, 51(6), 201 - 214. 10.15671/hjbc.1215813
Chicago Özcan Burcu,ERGÜN Betül,aybal kutlugun aysun,ergün mustafa çağrı,Aktürk Galip,Beyan Esin A Practical and Single-Use ITO-Pet Based Immunosensing Platform for Detection of Tumor Necrosis Factor-Alpha Biomarker. Hacettepe Journal of Biology and Chemistry 51, no.6 (2023): 201 - 214. 10.15671/hjbc.1215813
MLA Özcan Burcu,ERGÜN Betül,aybal kutlugun aysun,ergün mustafa çağrı,Aktürk Galip,Beyan Esin A Practical and Single-Use ITO-Pet Based Immunosensing Platform for Detection of Tumor Necrosis Factor-Alpha Biomarker. Hacettepe Journal of Biology and Chemistry, vol.51, no.6, 2023, ss.201 - 214. 10.15671/hjbc.1215813
AMA Özcan B,ERGÜN B,aybal kutlugun a,ergün m,Aktürk G,Beyan E A Practical and Single-Use ITO-Pet Based Immunosensing Platform for Detection of Tumor Necrosis Factor-Alpha Biomarker. Hacettepe Journal of Biology and Chemistry. 2023; 51(6): 201 - 214. 10.15671/hjbc.1215813
Vancouver Özcan B,ERGÜN B,aybal kutlugun a,ergün m,Aktürk G,Beyan E A Practical and Single-Use ITO-Pet Based Immunosensing Platform for Detection of Tumor Necrosis Factor-Alpha Biomarker. Hacettepe Journal of Biology and Chemistry. 2023; 51(6): 201 - 214. 10.15671/hjbc.1215813
IEEE Özcan B,ERGÜN B,aybal kutlugun a,ergün m,Aktürk G,Beyan E "A Practical and Single-Use ITO-Pet Based Immunosensing Platform for Detection of Tumor Necrosis Factor-Alpha Biomarker." Hacettepe Journal of Biology and Chemistry, 51, ss.201 - 214, 2023. 10.15671/hjbc.1215813
ISNAD Özcan, Burcu vd. "A Practical and Single-Use ITO-Pet Based Immunosensing Platform for Detection of Tumor Necrosis Factor-Alpha Biomarker". Hacettepe Journal of Biology and Chemistry 51/6 (2023), 201-214. https://doi.org/10.15671/hjbc.1215813