Investigation on the Binding properties of a Coumarin Derivative to Insulin by Spectroscopic and Computational Approaches
Yıl: 2023 Cilt: 51 Sayı: 3 Sayfa Aralığı: 297 - 307 Metin Dili: İngilizce DOI: 10.15671/hjbc.1272350 İndeks Tarihi: 17-10-2023
Investigation on the Binding properties of a Coumarin Derivative to Insulin by Spectroscopic and Computational Approaches
Öz: The binding properties of insulin hormone to the potential antidiabetic coumarin derivative umbelliferone (7hydroxycoumarin, 7HC) was investigated by absorption, fluorescence quenching and molecular docking methods. The negative signs of thermodynamic parameters (H and S) indicated that hydrogen bonds and van der Waals forces were dominant in the binding mode. The effect of common metal ions was investigated on binding parameters. According to the Förster’s theory; binding distance, r was obtained as 4.17 nm. The spectral data further supported by molecular docking calculations which show hydrogen bonds between 7HC and insulin.
Anahtar Kelime: Bir Kumarin Türevinin İnsüline Bağlanma Özelliklerinin Spektroskopik ve Hesaplamalı Yaklaşımlarla İncelenmesi
Öz: İnsülin hormonunun potansiyel antidiyabetik kumarin türevi olan umbelliferon (7hidroksikumarin, 7HC) ile bağlanma özel - likleri absorpsiyon, floresan söndürme ve moleküler kenetlenme yöntemleriyle incelenmiştir. Termodinamik parametre - lerin (H ve S) negatif işaretleri, hidrojen bağlarının ve van der Waals kuvvetlerinin bağlanma modunda baskın olduğunu göstermiştir. Bazı genel metal iyonlarının bağlanma parametreleri üzerindeki etkisi araştırılmıştır. Förster teorisine göre bağ uzunluğu, r, 4.17 nm olarak bulunmuştur. Spektral veriler, 7HC ve insülin arasındaki hidrojen bağlarını gösteren moleküler kenetlenme hesaplamaları ile desteklenmiştir.
Anahtar Kelime: Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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| APA | Gökoğlu E, Aklar B, TASKIN-TOK T (2023). Investigation on the Binding properties of a Coumarin Derivative to Insulin by Spectroscopic and Computational Approaches. , 297 - 307. 10.15671/hjbc.1272350 |
| Chicago | Gökoğlu Elmas,Aklar Buse,TASKIN-TOK Tugba Investigation on the Binding properties of a Coumarin Derivative to Insulin by Spectroscopic and Computational Approaches. (2023): 297 - 307. 10.15671/hjbc.1272350 |
| MLA | Gökoğlu Elmas,Aklar Buse,TASKIN-TOK Tugba Investigation on the Binding properties of a Coumarin Derivative to Insulin by Spectroscopic and Computational Approaches. , 2023, ss.297 - 307. 10.15671/hjbc.1272350 |
| AMA | Gökoğlu E,Aklar B,TASKIN-TOK T Investigation on the Binding properties of a Coumarin Derivative to Insulin by Spectroscopic and Computational Approaches. . 2023; 297 - 307. 10.15671/hjbc.1272350 |
| Vancouver | Gökoğlu E,Aklar B,TASKIN-TOK T Investigation on the Binding properties of a Coumarin Derivative to Insulin by Spectroscopic and Computational Approaches. . 2023; 297 - 307. 10.15671/hjbc.1272350 |
| IEEE | Gökoğlu E,Aklar B,TASKIN-TOK T "Investigation on the Binding properties of a Coumarin Derivative to Insulin by Spectroscopic and Computational Approaches." , ss.297 - 307, 2023. 10.15671/hjbc.1272350 |
| ISNAD | Gökoğlu, Elmas vd. "Investigation on the Binding properties of a Coumarin Derivative to Insulin by Spectroscopic and Computational Approaches". (2023), 297-307. https://doi.org/10.15671/hjbc.1272350 |
| APA | Gökoğlu E, Aklar B, TASKIN-TOK T (2023). Investigation on the Binding properties of a Coumarin Derivative to Insulin by Spectroscopic and Computational Approaches. Hacettepe Journal of Biology and Chemistry, 51(3), 297 - 307. 10.15671/hjbc.1272350 |
| Chicago | Gökoğlu Elmas,Aklar Buse,TASKIN-TOK Tugba Investigation on the Binding properties of a Coumarin Derivative to Insulin by Spectroscopic and Computational Approaches. Hacettepe Journal of Biology and Chemistry 51, no.3 (2023): 297 - 307. 10.15671/hjbc.1272350 |
| MLA | Gökoğlu Elmas,Aklar Buse,TASKIN-TOK Tugba Investigation on the Binding properties of a Coumarin Derivative to Insulin by Spectroscopic and Computational Approaches. Hacettepe Journal of Biology and Chemistry, vol.51, no.3, 2023, ss.297 - 307. 10.15671/hjbc.1272350 |
| AMA | Gökoğlu E,Aklar B,TASKIN-TOK T Investigation on the Binding properties of a Coumarin Derivative to Insulin by Spectroscopic and Computational Approaches. Hacettepe Journal of Biology and Chemistry. 2023; 51(3): 297 - 307. 10.15671/hjbc.1272350 |
| Vancouver | Gökoğlu E,Aklar B,TASKIN-TOK T Investigation on the Binding properties of a Coumarin Derivative to Insulin by Spectroscopic and Computational Approaches. Hacettepe Journal of Biology and Chemistry. 2023; 51(3): 297 - 307. 10.15671/hjbc.1272350 |
| IEEE | Gökoğlu E,Aklar B,TASKIN-TOK T "Investigation on the Binding properties of a Coumarin Derivative to Insulin by Spectroscopic and Computational Approaches." Hacettepe Journal of Biology and Chemistry, 51, ss.297 - 307, 2023. 10.15671/hjbc.1272350 |
| ISNAD | Gökoğlu, Elmas vd. "Investigation on the Binding properties of a Coumarin Derivative to Insulin by Spectroscopic and Computational Approaches". Hacettepe Journal of Biology and Chemistry 51/3 (2023), 297-307. https://doi.org/10.15671/hjbc.1272350 |
