Optimal conditions for production of extracellular protease from newly isolated Bacillus cereus strain CA15

YILMAZ, Ebru İnce; PORSUK, Ilknur; UYAR, Fikret; KIZIL, GÖKSEL

Optimal conditions for production of extracellular protease from newly isolated Bacillus cereus strain CA15

Fikret UYAR, (Dicle Üniversitesi, Fen Fakültesi, Biyoloji Bölümü, Diyarbakır, Türkiye)

Ilknur PORSUK, (Dicle Üniversitesi, Fen Fakültesi, Biyoloji Bölümü, Diyarbakır, Türkiye)

GÖKSEL KIZIL, (Dicle Üniversitesi, Fen Fakültesi, Kimya Bölümü, Diyarbakır, Türkiye)

Ebru İnce YILMAZ (Dicle Üniversitesi, Fen Edebiyat Fakültesi, Biyoloji Bölümü, Diyarbakır, Türkiye)

EurAsian Journal of BioSciences (elektronik)

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Yıl: 2011 Cilt: 0 Sayı: 5 Sayfa Aralığı: 1 - 9 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

Optimal conditions for production of extracellular protease from newly isolated Bacillus cereus strain CA15

Öz:

Topraktan alkalen proteaz üreticisi Bacillus sp. CA15 izole edildi. 16S ribosomal RNA gen dizilimine gore bu mikroorganizmanin Bacillus cereus ile çok yakin akraba oldugu tespit edildi. Proteaz üretiminin artisini saglamak için, kültür kosulari; karbon ve azot kaynaklari, metal iyonlari, pH ve isinin etkisi açisindan optimize edildi. En yüksek proteaz üretimi, %1 yagsiz süt tozu, %1 çözünür nisasta ve %0.6 $MgSO _4.7H _2O$ varliginda, baslangiç pH'si 8.0 ve 35°C'de üretilen kültürlerden elde edildi. Bu besiyerinde üretilen hücrelerin, canli hücre sayisinin, mL'de 1.8x108'e vardigi duragan fazda en iyi enzim aktivitesine rastlandi. Inorganik azot kaynaklari varliginda proteaz seviyesinin oldukça düsük oldugu bulundu. Sükroz ve laktozun varliginda da proteaz üretiminin azaldigi tespit edildi. Bacillus sp. CA15 alkalen proteazinin Triton X-100, Tween 20 and SDS'ye karsi son derece dayanikli oldugu gözlemlendi. Enzim aktivitesinin PMSF varliginda inhibe olmasi bu enzimin bir serin proteaz oldugunu düsündürdü.

Anahtar Kelime: kültür teknikleri proteaz optimizasyon kültür ortamı proteinaz ribozomal RNA enzim aktivitesi alkalin proteaz üretim izolasyon Bacillus cereus

Konular: Biyoloji

Yeni izole edilmis Bacillus cereus CA15 türünden ektraselüler proteaz üretimi için optimum kültür kosullari

Öz:

An alkaline protease producer Bacillus sp. strain CA15 was isolated from soil. The microorganism was found to be closely related to Bacillus cereus based on 16S ribosomal DNA sequencing. The culture conditions for higher protease production were optimized with respect to carbon and nitrogen sources, metal ions, pH and temperature. Maximum protease production was obtained in the medium supplemented with 1% skim milk, 1% starch and 0.6% $MgSO _4.7H _2O$, initial pH 8.0 at 35°C. The best enzyme production was obtained during the stationary phase in which the cell density reached to 1.8x108 cells/mL. The level of protease was found to be low in the presence of inorganic nitrogen sources. The protease production was diminished in the presence of sucrose and lactose. The extreme stability towards Triton X-100, Tween 20 and SDS was observed by Bacillus sp. CA15 alkaline protease. The enzyme activity was inhibited by PMSF suggested that presence of serine residues at the active sites.

Anahtar Kelime: proteinases ribosomal RNA enzyme activity alkaline protease production isolation Bacillus cereus culture techniques proteases optimization culture media

Konular: Biyoloji

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	UYAR F, PORSUK I, KIZIL G, YILMAZ E (2011). Optimal conditions for production of extracellular protease from newly isolated Bacillus cereus strain CA15. , 1 - 9.
Chicago	UYAR Fikret,PORSUK Ilknur,KIZIL GÖKSEL,YILMAZ Ebru İnce Optimal conditions for production of extracellular protease from newly isolated Bacillus cereus strain CA15. (2011): 1 - 9.
MLA	UYAR Fikret,PORSUK Ilknur,KIZIL GÖKSEL,YILMAZ Ebru İnce Optimal conditions for production of extracellular protease from newly isolated Bacillus cereus strain CA15. , 2011, ss.1 - 9.
AMA	UYAR F,PORSUK I,KIZIL G,YILMAZ E Optimal conditions for production of extracellular protease from newly isolated Bacillus cereus strain CA15. . 2011; 1 - 9.
Vancouver	UYAR F,PORSUK I,KIZIL G,YILMAZ E Optimal conditions for production of extracellular protease from newly isolated Bacillus cereus strain CA15. . 2011; 1 - 9.
IEEE	UYAR F,PORSUK I,KIZIL G,YILMAZ E "Optimal conditions for production of extracellular protease from newly isolated Bacillus cereus strain CA15." , ss.1 - 9, 2011.
ISNAD	UYAR, Fikret vd. "Optimal conditions for production of extracellular protease from newly isolated Bacillus cereus strain CA15". (2011), 1-9.

APA	UYAR F, PORSUK I, KIZIL G, YILMAZ E (2011). Optimal conditions for production of extracellular protease from newly isolated Bacillus cereus strain CA15. EurAsian Journal of BioSciences (elektronik), 0(5), 1 - 9.
Chicago	UYAR Fikret,PORSUK Ilknur,KIZIL GÖKSEL,YILMAZ Ebru İnce Optimal conditions for production of extracellular protease from newly isolated Bacillus cereus strain CA15. EurAsian Journal of BioSciences (elektronik) 0, no.5 (2011): 1 - 9.
MLA	UYAR Fikret,PORSUK Ilknur,KIZIL GÖKSEL,YILMAZ Ebru İnce Optimal conditions for production of extracellular protease from newly isolated Bacillus cereus strain CA15. EurAsian Journal of BioSciences (elektronik), vol.0, no.5, 2011, ss.1 - 9.
AMA	UYAR F,PORSUK I,KIZIL G,YILMAZ E Optimal conditions for production of extracellular protease from newly isolated Bacillus cereus strain CA15. EurAsian Journal of BioSciences (elektronik). 2011; 0(5): 1 - 9.
Vancouver	UYAR F,PORSUK I,KIZIL G,YILMAZ E Optimal conditions for production of extracellular protease from newly isolated Bacillus cereus strain CA15. EurAsian Journal of BioSciences (elektronik). 2011; 0(5): 1 - 9.
IEEE	UYAR F,PORSUK I,KIZIL G,YILMAZ E "Optimal conditions for production of extracellular protease from newly isolated Bacillus cereus strain CA15." EurAsian Journal of BioSciences (elektronik), 0, ss.1 - 9, 2011.
ISNAD	UYAR, Fikret vd. "Optimal conditions for production of extracellular protease from newly isolated Bacillus cereus strain CA15". EurAsian Journal of BioSciences (elektronik) 5 (2011), 1-9.