Karbosulfanın gökkuşağı alabalıkların (Oncorhynchus mykiss) eritrosit asetilkolinesteraz (AChE) enzim aktivitesine etkisi
Yıl: 2011 Cilt: 5 Sayı: 3 Sayfa Aralığı: 240 - 249 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022
Karbosulfanın gökkuşağı alabalıkların (Oncorhynchus mykiss) eritrosit asetilkolinesteraz (AChE) enzim aktivitesine etkisi
Öz: Enzim aktivitelerindeki değişimlerin tespiti, pestisitlerin ya da diğer zararlı kimyasalların tok-sik etkilerinin belirlenmesinde kullanılmaktadır. Karadeniz Bölgesinde kullanılan pestisitlerden karbosulfanın (250 g/L, EC) gökkuşağı alabalığı (Oncorhynchus mykiss)’nın enzim aktivitesi üzerine olan kronik etkileri araştırılmıştır. Bu amaçla ortama alıştırılan gökkuşağı alabalıkları (116.88 ±21.69 g ve 22.39 ±1.40 cm ) 60 gün süreyle akarsu sisteminde (6 L/h) karbosulfanın toksik etkisine maruz bırakılmıştır. Daha önceki denemelerden elde edilen veriler dikkate alı-narak, test balıklarının bulundukları ortamda karbosulfan miktarı 35 μg/L olacak şekilde dü-zenleme yapılmıştır. Kronik test süresince gökkuşağı alabalıklarının eritrosit asetilkolinesteraz (AChE) aktivitesi ölçülerek inhibisyon oranı tespit edilmiştir. Gökkuşağı alabalıkların enzim aktivitelerindeki değişimin istatistiksel olarak önemli olduğu saptanmıştır (p<0.05). AChE in-hibisyon oranındaki artış 3. haftaya kadar sürdüğü ve inhibisyon oranının % 41.32 olduğu tes-pit edilmiştir. Enzim aktivitesindeki değişimin balıkların davranışları üzerine etkili olduğu da belirlenmiştir.
Anahtar Kelime: Effects of carbosulfan on erythrocyte acetylcholinesterase (AChE) activities of rainbow trout (Oncorhynchus mykiss)
Öz: Detection of changes in enzyme activities has been used as a method to determine the toxic ef-fects of pesticides or other toxic chemical. In this study, chronic effects of pesticide carbosul-fan (250 g/L, EC) used commonly in the Eastern Black Sea Region on enzyme activity of rain-bow trout (Oncorhynchus mykiss) were determined. For this purpose, rainbow trout (116.88 ±21.69 g and 22.39 ±1.40 cm) were acclimated to the laboratory conditions and exposed to carbosulfan for 60 days in flow-throuhgt system (6 L/h). According to data of preliminary tests, carbosulfan concentration of test water was designed as 35 μg/L. During the chronic tests, erythrocyte acetylcholinesterase (AChE) activity of rainbow trout was measured and inhibition rate of enzyme activity was determined. Changes in enzyme activity of rainbow trout was sta-tistically significant (p<0.05). Increase of inhibition rate on AChE activity was up to 3rd week. Inhibition rates of AChE was determined as 41.32 % and it was determined that change on enzyme activity affected fish behavior.
Anahtar Kelime: Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
- AFS-FHS, (2003). American Fisheries Society-Fish Health Section. Suggested Procedures for the Detection and Identification of Cer-tain Finfish and Shellfish Pathogens, fifthed. Fish Health Section, American Fisheries So-ciety, Bethesda, MD, USA.
- Altinok, I., Capkin, E. Karahan, S., Boran, M., (2006). Effects of water quality and fish size on toxicity of methiocarb, a carbamate pesti-cide, to rainbow trout, Environmental Toxi-cology and Pharmacology, 22: 20-26. doi:10.1016/j.etap.2005.11.002
- Anon, (1994). International Programme On Chemical Safety, Environmental Health Criteria 153. http://www.inchem.org/documents/ehc/ehc/ehc153.htm
- Behra, M., Cousin, X., Bertrand, C., Vonesch, J.L., Biellmann, D., Chatonnet, A., Strahle, U., (2002). Acetylcholinesterase is required for neuronal and muscular development in the zebrafish embryo, Nature Neuroscience, 5: 111-118. doi:10.1038/nn788
- Burgeot, T., Bocquene, G., Porte, C., Dimeet, J., Santella, R.M., Garcia de la Parra, L.M., Pfhol-Leszkowicz, A., Raoux, C., Galgani, F., (1996). Bioindicators of pollutant expo-sure in the Northwestern Mediterranean Sea, Marine Ecology Progress Series, 131: 125–141. doi:10.3354/meps131125
- Capkin, E., Altinok, I., Karahan, S., (2006). Water quality and fish size affect toxicity of endosulfan, an organochlorine pesticide, to rainbow trout, Chemosphere, 64: 1793-1800. doi:10.1016/j.chemosphere.2005.12.050
- Casillas, E., Meyers, M., Ames, W., (1983). Re-lationship of serum chemistry values to liver and kidney histopathology in English sole (Parophrys vetulus) after acute exposure to carbon tetrachloride, Aquatic Toxicology, 3: 61-78. doi:10.1016/0166-445X(83)90007-3
- Doğan, S., (2006). The in vitro effects of some pesticides on carbonic anhydrase activity of Oncorhynchus mykiss and Cyprinus carpio carpio fish, Journal of Hazardous Materials A, 132: 171-176.
- Dutta, H.M, Arends, D.A., (2003). Effects of en-dosulfan on brain acetylcholinesterase acti-vity in juvenile bluegill sunfish, Environ-menta Research, 91(3): 157-62. doi:10.1016/S0013-9351(02)00062-2
- Dutta, H.M., Marcelino, J., Richmonds, C., (1992). Brain acetylcholinesterase activity and optomotor behavior in bluegills, Lepo-mis macrochirus exposed to different con-centrations of diazinon, Arch Int Physiol Bi-ochim Biophys, 100(5): 331-334. doi:10.3109/13813459209000721
- Ellman, G.L., Courtney, D.K., Andres Jr., V., Fe-atherstone, R.M., (1961). A new rapid colo-rimetric determination of acetylcholineste-rase activity, Biochemical Pharmacology, 7: 88-95. doi:10.1016/0006-2952(61)90145-9
- Escartin, E., Porte, C., (1996). Acetylcholineste-rase inhibition in the crayfish procarambus clarkii exposed to fenitrothion, Ecotoxico-logy and Environmental Safety, 34: 160-164. doi:10.1006/eesa.1996.0058
- Ferrando, M.D., Sancho, E. and Moliner, E.A., (1991). Comparative Acute Toxicities of Selected Pesticides to Anguilla anguilla, Journal of Environmental Science and He- alth B, 26(5-6): 491-8. doi:10.1080/03601239109372751
- Fisher, S.W., (1991). Changes in the toxicity of the three pesticides as afunction of environ-mental pH and temperature, Bulletin of En-vironmental Contamination and Toxicology, 46: 197-202. doi:10.1007/BF01691937
- Halappa, R., David, M., (2009). Behavioural Responses of the Freshwater Fish, Cyprinus carpio (Linnaeus) Following Sublethal Exposure to Chlorpyrifos, Turkish Journal of Fisheries and Aquatic Sciences, 9: 233-238. doi:10.4194/trjfas.2009.0218
- Hanneman, E., Westerfield, M., (1989). Early expression of acetylcholinesterase activity in functionally distinct neurons of the zebra-fish, Journal of Comparative Neurology, 284: 350-361. doi:10.1002/cne.902840303
- Huang, T.L., Obih, P.O., Jaiswal, R., Hartley, W.R., Thiyagarajah, A., (1997). Evaluation of liver and brain esterases in the spotted gar Wsh (Lepisosteus oculatus) as biomarkers of eVect in the lower Mississippi River basin, Bulletin of Environmental Contamination and Toxicology, 58: 688-695. doi:10.1007/s001289900388
- Küster, E., (2005). Cholin- and carboxylesterase activities in developing zebrafish embryos (Danio rerio) and their potential use for in-secticide hazard assessment, Aquatic Toxi-cology, 75: 76-85. doi:10.1016/j.aquatox.2005.07.005
- Minier, C., Levy, F., Rabel, D., Bocquene, G., Godefroy, D., Burgeot, T., Leboulenger, F., (2000). Flounder health status in the Seine Bay. A multibiomarker study, Marine Envi-ronmental Research, 50: 373-377. doi:10.1016/S0141-1136(00)00059-3
- Noriega, G.O., Gonzales, S., Tomaro, M.L., Batle, A.M., (2002). Paraquat-generated oxidative stress in rat liver induces he-meoxygenase- 1 and aminolevulinic acid synthase, Free Radical Research, 36: 633-639. doi:10.1080/10715760290029065
- OECD, (1992). Organization for economic coo-peration and development, guideline for the testing of chemicals: fish, acute toxicity test, No: 203.
- PAN, (2010). Pesticide Action Network, Pesti-cide Database, North America (San Fran-cisco, CA). www.pesticideinfo.org
- Parron, T., Hern’andez, A.F., Pla, A., Villanueva, E., (1996). Clinical and biochemical changes in greenhouse sprayers chronically exposed to pesticides, Human and Experimental Toxicology, 15: 957-963. doi:10.1177/096032719601501203
- Richmonds, R.C. and Dutta, H.M., (1992a). Ef-fect of Malathion on the Optomotor Beha-vior of Bluegill Sunfish, Lepomis macrochi-rus, Comparative Biochemistry and Physio-logy, 102(3): 523-26. doi:10.1016/0742-8413(92)90153-X
- Richmonds, R.C., Dutta, H.M., (1992b). Effect of malathion on the brain acetylcholinesterase activity of bluegill sunfish Lepomis macroc-hirus, Bulletin of Environmental Contami-nation and Toxicology, 49: 431-435. doi:10.1007/BF01239648
- Salles, J.B., Cunha Bastos, V.L.F., Silva Filho, M.V., Machado, O.L.T., Salles, C.M.C., Gi-ovanni de Simone, S., Cunha Bastos, J., (2006). A novel butyrylcholinesterase from serum of Leporinus macrocephalus, a Neot-ropical fish, Biochimie, 88: 59-68. doi:10.1016/j.biochi.2005.06.017
- Sao A., Pillai A.K., Gupta V.K., (2008). Spectro-fotometric determination of carbosulfan in environmental samples, Journal of Scientifi and Industrial Research, 67: 1088-1091.
- Snejdarkova, M., Svobodova, L., Evtugyn G., Budnikov, H., Karyakin, A., Nikolelis, D.P., Hianik, T., (2004). Acetylcholinesterase sensors based on gold electrodes modified with dendrimer and polyaniline a compara-tive research, Analytica Chimica Acta, 514: 79-88.doi:10.1016/j.aca.2004.03.019
- Sturn, A., Silva de Assis, H.C., Hansen, P.D., (1999). Cholinesterases of marine teleost fish: enzymological characterization and potential use in the monitoring of neurotoxic contamination, Marine Environmental Rese-arch, 47: 389-398. doi:10.1016/S0141-1136(98)00127-5
- Varo, I., Navarro, J.C., Amat, F., Guilhermino, L., (2003). Effect of dichlorvos on choli-nesterase activity of the European sea bass (Dicentrarchus labrax), Pesticide Bioche- mistry and Physiology, 75: 61-72. doi:10.1016/S0048-3575(03)00019-1
- Wang, L.Z., Gorlin, J., Michaud, S. E., Janmey, P.A., Goddeau, R.P., Kuuse, R., Uibo, R., Adams, D., Sawyer, E.S., (2000). Purifica-tion of Salmon Clotting Factors and Their Use as Tissue Sealants, Thrombosis Rese-arch, 100 (6), 537-548. doi:10.1016/S0049-3848(00)00362-5
- Weiss, C.M., (1959). Response of fish to sub-let-hal exposures of organic phosphorus insecti-cides, Sewage and Industrial Wastes, 31: 580-593.
- WHO, (1993). Methyl Parathion (Environmental Health Criteria-EHC 145, 1992). Internatio-nal Programme on Chemical Safety, Envi-ron. Health Crit. 145. World Health Organi-zation, Geneva, pp. 1-135.
| APA | ÇAPKIN E (2011). Karbosulfanın gökkuşağı alabalıkların (Oncorhynchus mykiss) eritrosit asetilkolinesteraz (AChE) enzim aktivitesine etkisi. , 240 - 249. |
| Chicago | ÇAPKIN Erol Karbosulfanın gökkuşağı alabalıkların (Oncorhynchus mykiss) eritrosit asetilkolinesteraz (AChE) enzim aktivitesine etkisi. (2011): 240 - 249. |
| MLA | ÇAPKIN Erol Karbosulfanın gökkuşağı alabalıkların (Oncorhynchus mykiss) eritrosit asetilkolinesteraz (AChE) enzim aktivitesine etkisi. , 2011, ss.240 - 249. |
| AMA | ÇAPKIN E Karbosulfanın gökkuşağı alabalıkların (Oncorhynchus mykiss) eritrosit asetilkolinesteraz (AChE) enzim aktivitesine etkisi. . 2011; 240 - 249. |
| Vancouver | ÇAPKIN E Karbosulfanın gökkuşağı alabalıkların (Oncorhynchus mykiss) eritrosit asetilkolinesteraz (AChE) enzim aktivitesine etkisi. . 2011; 240 - 249. |
| IEEE | ÇAPKIN E "Karbosulfanın gökkuşağı alabalıkların (Oncorhynchus mykiss) eritrosit asetilkolinesteraz (AChE) enzim aktivitesine etkisi." , ss.240 - 249, 2011. |
| ISNAD | ÇAPKIN, Erol. "Karbosulfanın gökkuşağı alabalıkların (Oncorhynchus mykiss) eritrosit asetilkolinesteraz (AChE) enzim aktivitesine etkisi". (2011), 240-249. |
| APA | ÇAPKIN E (2011). Karbosulfanın gökkuşağı alabalıkların (Oncorhynchus mykiss) eritrosit asetilkolinesteraz (AChE) enzim aktivitesine etkisi. Journal of FisheriesSciences.com, 5(3), 240 - 249. |
| Chicago | ÇAPKIN Erol Karbosulfanın gökkuşağı alabalıkların (Oncorhynchus mykiss) eritrosit asetilkolinesteraz (AChE) enzim aktivitesine etkisi. Journal of FisheriesSciences.com 5, no.3 (2011): 240 - 249. |
| MLA | ÇAPKIN Erol Karbosulfanın gökkuşağı alabalıkların (Oncorhynchus mykiss) eritrosit asetilkolinesteraz (AChE) enzim aktivitesine etkisi. Journal of FisheriesSciences.com, vol.5, no.3, 2011, ss.240 - 249. |
| AMA | ÇAPKIN E Karbosulfanın gökkuşağı alabalıkların (Oncorhynchus mykiss) eritrosit asetilkolinesteraz (AChE) enzim aktivitesine etkisi. Journal of FisheriesSciences.com. 2011; 5(3): 240 - 249. |
| Vancouver | ÇAPKIN E Karbosulfanın gökkuşağı alabalıkların (Oncorhynchus mykiss) eritrosit asetilkolinesteraz (AChE) enzim aktivitesine etkisi. Journal of FisheriesSciences.com. 2011; 5(3): 240 - 249. |
| IEEE | ÇAPKIN E "Karbosulfanın gökkuşağı alabalıkların (Oncorhynchus mykiss) eritrosit asetilkolinesteraz (AChE) enzim aktivitesine etkisi." Journal of FisheriesSciences.com, 5, ss.240 - 249, 2011. |
| ISNAD | ÇAPKIN, Erol. "Karbosulfanın gökkuşağı alabalıkların (Oncorhynchus mykiss) eritrosit asetilkolinesteraz (AChE) enzim aktivitesine etkisi". Journal of FisheriesSciences.com 5/3 (2011), 240-249. |
