Objective: This study was conducted to determine the barriers and facilitators of research utilization among midwives and nurses working in gynecology and obstetrics clinics. Methods: This descriptive and cross-sectional study was conducted with 85 midwives and nurses working in the obstetrics and gynecology clinics and polyclinics of a training and research hospital in the south of Turkey. Data were collected between June and December 2020, using the Personal Information Form and the Research Use Barriers Scale (RUBS). Data were collected based on the self-report of midwives and nurses. Data were analyzed using descriptive statistics, Kolmogorov-Smirnov normal distribution test, the Kruskall-Wallis H test, and the Mann-Whitney U test. Results: It was determined that midwives and nurses had a high mean RUBS score (91.22±15.30). It was determined that the highest perception of obstacles related to the use of research belonged to the sub-dimensions of "Institution" and "Nurse", and the lowest perception of obstacles belonged to the sub-dimensions of "Research" and "Presentation". 33% of midwives and nurses stated that providing access to resources is the most important facilitating factor in research use. It was found that there was no statistically significant difference between the total mean score of midwives and nurses on RUBS and all other descriptive characteristics except education. Conclusion: In the study, it was determined that midwives and nurses working in the field of gynecology and obstetrics had a high perception of barriers to using the research results. Strategies should be developed to make it easier for midwives and nurses to access research results and use them in practice (resources, internet access, etc.). For this purpose, in-house activities should be carried out to create a culture of research and evidence-based practice in the field of obstetrics and gynecology. In this context, it is important to ensure university-hospital cooperation.