Assessment of the Bioactivity of Zinc Nanoparticles Synthesized Using Erica arborea Plant
Yıl: 2023 Cilt: 8 Sayı: 3 Sayfa Aralığı: 361 - 366 Metin Dili: İngilizce DOI: 10.35229/jaes.1301513 İndeks Tarihi: 26-10-2023
Assessment of the Bioactivity of Zinc Nanoparticles Synthesized Using Erica arborea Plant
Öz: The synthesis of nanoparticles by environmentally friendly, cost-effective and simple methods has been the subject of much research in medicine and industry. In particular, it offers a promising approach for the development of next-generation nano-based drugs. This study synthesized zinc oxide nanoparticles (ZnO NPs) using Erica arborea (E. arborea) leaf extract as a stabilizing and reducing agent. The prepared ZnO NPs were characterized by UV-vis, FTIR and SEM-EDX analysis. SEM-EDX analysis showed that the nanoparticles were spherical in shape and showed strong signals of zinc metal. UV-vis analysis confirmed that the color of the solution changed to light yellow, indicating the formation of nanoparticles, and exhibited an absorption peak at 350 nm. FT-IR confirmed that the formation of nanoparticles was accompanied by metabolites of the leaf extract. The pharmacological potential of zinc nanoparticles synthesized by E. arborea was evaluated by antioxidant (DPPH method), antimicrobial (disk diffusion method) and lipid peroxidation (TBA method) activities. The biosynthesized ZnO NPs exhibited better antioxidant activity compared to the plant extract. The IC50 values of DPPH radical scavenging activity of ZnO NPs and leaf extract were 18.71±0.35 and 9.21±0.10, respectively. The lipid peroxidation inhibitory activity IC50 value of ZnO NPs was found to be 5.33±0.09. ZnO NPs were found to be sensitive against pathogenic bacteria P. aeruginosa, B. cereus, B. subtilis and S. aureus and fungal strain C. albicans. The findings suggest that synthesizing naturally synthesized zinc nanoparticles could be an alternative agent to physical and chemical methods. In addition, the pharmacological activity of nanoparticles will contribute to next-generation drug development studies.
Anahtar Kelime: Erica arborea Bitkisi Kullanılarak Sentezlenen Çinko Nanopartiküllerin Biyoaktivitesinin Değerlendirilmesi
Öz: Nanopartiküller (NP’ler), çevre dostu, uygun maliyetli ve basit yöntemlerle sentezi, tıp ve endüstri alanlarında pek çok araştırmaya konu olmuştur. NP’ler özellikle yeni nesil nano tabanlı ilaçların geliştirilmesi için umut verici bir yaklaşım sunmaktadır. Bu çalışmada, Erica arborea (E. arborea) yaprak özütü stabilize edici ve indirgeyici ajan olarak kullanıldı ve çinko oksit NP’ler (ZnO NP’ler) sentezlendi. Hazırlanan ZnO NP’ler ultraviyole-görünür spektroskopisi (UV-vis), fourier dönüşümlü kızılötesi spektroskopisi (FTIR), taramalı elektron mikroskopisi (SEM) and enerji dağılımlı X-ışını spektroskopisi (EDX) analizleri ile karakterize edildi. SEM ve EDX analizlerinde, NP’lerin küresel şekilde olduğu ve çinko metalinin güçlü sinyalleri görüldü. UV-vis analizi, çözeltinin renginin açık sarıya dönüşüyle NP'lerin oluşumunu doğruladı ve 350 nm'de bir absorpsiyon piki sergiledi. FT-IR, NP'lerin oluşumuna yaprak ekstresinin metabolitlerinin eşlik ettiğini doğruladı. E. arborea tarafından sentezlenen ZnO NP'lerin biyolojik aktivitesi antimikrobiyal, antioksidan ve lipid peroksidasyon analizleri ile gerçekleştirildi. Biyojenik ZnO NP’ler, Pseudomonas aeruginosa, Bacillus cereus, Bacillus subtilis ve Staphylococcus aureus patojen bakterileri ve Candida albicans mantarına karşı duyarlı olduğu bulundu. Özellikle Bacillus subtilis ve Candida albicans rifampin antibiyotiğine göre daha etkiliydi. ZnO NP’ler, bitki ekstraktına kıyasla daha iyi antioksidan aktivite sergiledi. ZnO NP'ler ve yaprak ekstraktının DPPH radikal süpürme aktivitesinin IC50 değerleri sırasıyla 9.21±0.10 ve 18.71±0.35 idi. Ayrıca ZnO NP'lerin lipid peroksidasyon inhibitör aktivitesi için IC50 değeri 5.33±0.09 olarak bulunmuştur. Bulgular, doğal olarak sentezlenen çinko NP’lerin sentezlenmesi, fiziksel ve kimyasal yöntemlere alternatif bir ajan olabileceğini göstermektedir. Ayrıca NP’lerin biyolojik aktivitesi yeni nesil ilaç geliştirme çalışmalarına katkı sağlayacaktır.
Anahtar Kelime: Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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| APA | KOÇAK Y, Meydan I (2023). Assessment of the Bioactivity of Zinc Nanoparticles Synthesized Using Erica arborea Plant. , 361 - 366. 10.35229/jaes.1301513 |
| Chicago | KOÇAK YILMAZ,Meydan Ismet Assessment of the Bioactivity of Zinc Nanoparticles Synthesized Using Erica arborea Plant. (2023): 361 - 366. 10.35229/jaes.1301513 |
| MLA | KOÇAK YILMAZ,Meydan Ismet Assessment of the Bioactivity of Zinc Nanoparticles Synthesized Using Erica arborea Plant. , 2023, ss.361 - 366. 10.35229/jaes.1301513 |
| AMA | KOÇAK Y,Meydan I Assessment of the Bioactivity of Zinc Nanoparticles Synthesized Using Erica arborea Plant. . 2023; 361 - 366. 10.35229/jaes.1301513 |
| Vancouver | KOÇAK Y,Meydan I Assessment of the Bioactivity of Zinc Nanoparticles Synthesized Using Erica arborea Plant. . 2023; 361 - 366. 10.35229/jaes.1301513 |
| IEEE | KOÇAK Y,Meydan I "Assessment of the Bioactivity of Zinc Nanoparticles Synthesized Using Erica arborea Plant." , ss.361 - 366, 2023. 10.35229/jaes.1301513 |
| ISNAD | KOÇAK, YILMAZ - Meydan, Ismet. "Assessment of the Bioactivity of Zinc Nanoparticles Synthesized Using Erica arborea Plant". (2023), 361-366. https://doi.org/10.35229/jaes.1301513 |
| APA | KOÇAK Y, Meydan I (2023). Assessment of the Bioactivity of Zinc Nanoparticles Synthesized Using Erica arborea Plant. JOURNAL OF ANATOLIAN ENVIRONMENTAL AND ANIMAL SCIENCES, 8(3), 361 - 366. 10.35229/jaes.1301513 |
| Chicago | KOÇAK YILMAZ,Meydan Ismet Assessment of the Bioactivity of Zinc Nanoparticles Synthesized Using Erica arborea Plant. JOURNAL OF ANATOLIAN ENVIRONMENTAL AND ANIMAL SCIENCES 8, no.3 (2023): 361 - 366. 10.35229/jaes.1301513 |
| MLA | KOÇAK YILMAZ,Meydan Ismet Assessment of the Bioactivity of Zinc Nanoparticles Synthesized Using Erica arborea Plant. JOURNAL OF ANATOLIAN ENVIRONMENTAL AND ANIMAL SCIENCES, vol.8, no.3, 2023, ss.361 - 366. 10.35229/jaes.1301513 |
| AMA | KOÇAK Y,Meydan I Assessment of the Bioactivity of Zinc Nanoparticles Synthesized Using Erica arborea Plant. JOURNAL OF ANATOLIAN ENVIRONMENTAL AND ANIMAL SCIENCES. 2023; 8(3): 361 - 366. 10.35229/jaes.1301513 |
| Vancouver | KOÇAK Y,Meydan I Assessment of the Bioactivity of Zinc Nanoparticles Synthesized Using Erica arborea Plant. JOURNAL OF ANATOLIAN ENVIRONMENTAL AND ANIMAL SCIENCES. 2023; 8(3): 361 - 366. 10.35229/jaes.1301513 |
| IEEE | KOÇAK Y,Meydan I "Assessment of the Bioactivity of Zinc Nanoparticles Synthesized Using Erica arborea Plant." JOURNAL OF ANATOLIAN ENVIRONMENTAL AND ANIMAL SCIENCES, 8, ss.361 - 366, 2023. 10.35229/jaes.1301513 |
| ISNAD | KOÇAK, YILMAZ - Meydan, Ismet. "Assessment of the Bioactivity of Zinc Nanoparticles Synthesized Using Erica arborea Plant". JOURNAL OF ANATOLIAN ENVIRONMENTAL AND ANIMAL SCIENCES 8/3 (2023), 361-366. https://doi.org/10.35229/jaes.1301513 |
